Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatment
IntroductionDiabetic retinopathy is a significant microvascular disorder and the leading cause of vision impairment in working-age individuals. Hyperglycemia triggers retinal damage through mechanisms such as the polyol pathway and the accumulation of advanced glycation end products (AGEs). Inhibiti...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1513967/full |
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| author | Saheem Ahmad Mohammad Faizan Ali Ahmad Saif Khan Sultan Alouffi Mahvish Khan Mohd Wajid Ali Khan Irfan Ahmad Ansari |
| author_facet | Saheem Ahmad Mohammad Faizan Ali Ahmad Saif Khan Sultan Alouffi Mahvish Khan Mohd Wajid Ali Khan Irfan Ahmad Ansari |
| author_sort | Saheem Ahmad |
| collection | DOAJ |
| description | IntroductionDiabetic retinopathy is a significant microvascular disorder and the leading cause of vision impairment in working-age individuals. Hyperglycemia triggers retinal damage through mechanisms such as the polyol pathway and the accumulation of advanced glycation end products (AGEs). Inhibiting key enzymes in this pathway, aldose reductase (AR) and sorbitol dehydrogenase (SD), alongside preventing AGE formation, may offer therapeutic strategies for diabetic retinopathy and other vascular complications. This study investigates the ability of Ducrosia anethifolia, an Arabian plant, to inhibit AR and SD enzymes.MethodsMethanolic extracts of the plant were tested in enzyme assays and further analyzed using Lineweaver-Burk plots for kinetic insights. Additionally, the effects on AGE production and sorbitol accumulation in red blood cells were evaluated.ResultsThe methanolic extract showed strong inhibitory effects on AR (IC50: 69.41 ± 3.59 μg/mL) and SD (IC50: 31.11 ± 5.58 μg/mL), acting through a mixed-inhibition mechanism. It also significantly reduced sorbitol accumulation and AGE formation.DiscussionThese findings suggest that the extract’s inhibition of the polyol pathway enzymes is due to its phytochemical content. Further isolation and identification of these active compounds could provide valuable insights for developing future pharmaceutical treatments for diabetic retinopathy. |
| format | Article |
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| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-499fb8b2b648493ea3fc7fc20b37f7402024-12-23T08:59:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-12-011510.3389/fphar.2024.15139671513967Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatmentSaheem Ahmad0Mohammad Faizan Ali Ahmad1Saif Khan2Sultan Alouffi3Mahvish Khan4Mohd Wajid Ali Khan5Irfan Ahmad Ansari6Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail, Saudi ArabiaDepartment of Biosciences, Integral University, Lucknow, IndiaDepartment of Basic Dental and Medical Sciences, College of Dentistry, University of Hail, Hail, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, Hail, Saudi ArabiaDepartment of Chemistry, College of Science, University of Hail, Hail, Saudi ArabiaDepartment of Biosciences, Integral University, Lucknow, IndiaIntroductionDiabetic retinopathy is a significant microvascular disorder and the leading cause of vision impairment in working-age individuals. Hyperglycemia triggers retinal damage through mechanisms such as the polyol pathway and the accumulation of advanced glycation end products (AGEs). Inhibiting key enzymes in this pathway, aldose reductase (AR) and sorbitol dehydrogenase (SD), alongside preventing AGE formation, may offer therapeutic strategies for diabetic retinopathy and other vascular complications. This study investigates the ability of Ducrosia anethifolia, an Arabian plant, to inhibit AR and SD enzymes.MethodsMethanolic extracts of the plant were tested in enzyme assays and further analyzed using Lineweaver-Burk plots for kinetic insights. Additionally, the effects on AGE production and sorbitol accumulation in red blood cells were evaluated.ResultsThe methanolic extract showed strong inhibitory effects on AR (IC50: 69.41 ± 3.59 μg/mL) and SD (IC50: 31.11 ± 5.58 μg/mL), acting through a mixed-inhibition mechanism. It also significantly reduced sorbitol accumulation and AGE formation.DiscussionThese findings suggest that the extract’s inhibition of the polyol pathway enzymes is due to its phytochemical content. Further isolation and identification of these active compounds could provide valuable insights for developing future pharmaceutical treatments for diabetic retinopathy.https://www.frontiersin.org/articles/10.3389/fphar.2024.1513967/fulldiabetic retinopathypolyol pathwayadvanced glycation end products (AGEs)aldose reductase (AR)sorbitol dehydrogenase (SD) |
| spellingShingle | Saheem Ahmad Mohammad Faizan Ali Ahmad Saif Khan Sultan Alouffi Mahvish Khan Mohd Wajid Ali Khan Irfan Ahmad Ansari Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatment Frontiers in Pharmacology diabetic retinopathy polyol pathway advanced glycation end products (AGEs) aldose reductase (AR) sorbitol dehydrogenase (SD) |
| title | Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatment |
| title_full | Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatment |
| title_fullStr | Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatment |
| title_full_unstemmed | Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatment |
| title_short | Inhibition of the polyol pathway by Ducrosia anethifolia extract: plausible implications for diabetic retinopathy treatment |
| title_sort | inhibition of the polyol pathway by ducrosia anethifolia extract plausible implications for diabetic retinopathy treatment |
| topic | diabetic retinopathy polyol pathway advanced glycation end products (AGEs) aldose reductase (AR) sorbitol dehydrogenase (SD) |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1513967/full |
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