Opportunities and challenges of bacterial extracellular vesicles in regenerative medicine
Abstract Extracellular vesicles (EVs) are membrane-bound vesicles that are shed or secreted from the cell membrane and enveloped by a lipid bilayer. They possess stability, low immunogenicity, and non-cytotoxicity, exhibiting extensive prospects in regenerative medicine (RM). However, natural EVs po...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-024-02935-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559136336084992 |
|---|---|
| author | Jiming Guo Zhijie Huang Qinjing Wang Min Wang Yue Ming Weixing Chen Yisheng Huang Zhengming Tang Mingshu Huang Hongyu Liu Bo Jia |
| author_facet | Jiming Guo Zhijie Huang Qinjing Wang Min Wang Yue Ming Weixing Chen Yisheng Huang Zhengming Tang Mingshu Huang Hongyu Liu Bo Jia |
| author_sort | Jiming Guo |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) are membrane-bound vesicles that are shed or secreted from the cell membrane and enveloped by a lipid bilayer. They possess stability, low immunogenicity, and non-cytotoxicity, exhibiting extensive prospects in regenerative medicine (RM). However, natural EVs pose challenges, such as insufficient targeting capabilities, potential biosafety concerns, and limited acquisition pathways. Although engineered EVs demonstrate excellent therapeutic efficacy, challenges such as low production yield and the complexity of engineering modifications constrain their further clinical applications. Bacteria have advantages such as rapid proliferation, diverse gene editing methods, mature cultivation techniques, and relatively easy preparation of bacterial EVs (BEVs), which can be used to effectively address the challenges currently encountered in the field of EVs. This review provides a description of the biogenesis and pathophysiological functions of BEVs, and strategies for optimizing BEVs preparation to attain efficiency and safety are discussed. An analysis of natural characteristics of BEVs is also conducted to explore how to leverage their advantages or mitigate their limitations, thereby overcoming constraints on the application of BEVs in RM. In summary, engineered BEVs possess characteristics such as high production yield, excellent stability, and high drug-delivering capabilities, laying the foundation for their application in RM. Graphical Abstract |
| format | Article |
| id | doaj-art-495c70af61e943b393a016e781b2a82d |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-495c70af61e943b393a016e781b2a82d2025-01-05T12:45:04ZengBMCJournal of Nanobiotechnology1477-31552025-01-0123112710.1186/s12951-024-02935-1Opportunities and challenges of bacterial extracellular vesicles in regenerative medicineJiming Guo0Zhijie Huang1Qinjing Wang2Min Wang3Yue Ming4Weixing Chen5Yisheng Huang6Zhengming Tang7Mingshu Huang8Hongyu Liu9Bo Jia10Stomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityStomatological Hospital, School of Stomatology, Southern Medical UniversityAbstract Extracellular vesicles (EVs) are membrane-bound vesicles that are shed or secreted from the cell membrane and enveloped by a lipid bilayer. They possess stability, low immunogenicity, and non-cytotoxicity, exhibiting extensive prospects in regenerative medicine (RM). However, natural EVs pose challenges, such as insufficient targeting capabilities, potential biosafety concerns, and limited acquisition pathways. Although engineered EVs demonstrate excellent therapeutic efficacy, challenges such as low production yield and the complexity of engineering modifications constrain their further clinical applications. Bacteria have advantages such as rapid proliferation, diverse gene editing methods, mature cultivation techniques, and relatively easy preparation of bacterial EVs (BEVs), which can be used to effectively address the challenges currently encountered in the field of EVs. This review provides a description of the biogenesis and pathophysiological functions of BEVs, and strategies for optimizing BEVs preparation to attain efficiency and safety are discussed. An analysis of natural characteristics of BEVs is also conducted to explore how to leverage their advantages or mitigate their limitations, thereby overcoming constraints on the application of BEVs in RM. In summary, engineered BEVs possess characteristics such as high production yield, excellent stability, and high drug-delivering capabilities, laying the foundation for their application in RM. Graphical Abstracthttps://doi.org/10.1186/s12951-024-02935-1Bacterial extracellular vesiclesOuter membrane vesiclesEngineering strategyRegenerative medicineDrug delivery |
| spellingShingle | Jiming Guo Zhijie Huang Qinjing Wang Min Wang Yue Ming Weixing Chen Yisheng Huang Zhengming Tang Mingshu Huang Hongyu Liu Bo Jia Opportunities and challenges of bacterial extracellular vesicles in regenerative medicine Journal of Nanobiotechnology Bacterial extracellular vesicles Outer membrane vesicles Engineering strategy Regenerative medicine Drug delivery |
| title | Opportunities and challenges of bacterial extracellular vesicles in regenerative medicine |
| title_full | Opportunities and challenges of bacterial extracellular vesicles in regenerative medicine |
| title_fullStr | Opportunities and challenges of bacterial extracellular vesicles in regenerative medicine |
| title_full_unstemmed | Opportunities and challenges of bacterial extracellular vesicles in regenerative medicine |
| title_short | Opportunities and challenges of bacterial extracellular vesicles in regenerative medicine |
| title_sort | opportunities and challenges of bacterial extracellular vesicles in regenerative medicine |
| topic | Bacterial extracellular vesicles Outer membrane vesicles Engineering strategy Regenerative medicine Drug delivery |
| url | https://doi.org/10.1186/s12951-024-02935-1 |
| work_keys_str_mv | AT jimingguo opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT zhijiehuang opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT qinjingwang opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT minwang opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT yueming opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT weixingchen opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT yishenghuang opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT zhengmingtang opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT mingshuhuang opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT hongyuliu opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine AT bojia opportunitiesandchallengesofbacterialextracellularvesiclesinregenerativemedicine |