Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy
Basal cell carcinoma (BCC) is a common type of skin cancer. Conventional approaches to BCC diagnosis often involve invasive histological examinations that can distort or even destroy information derived from the biomolecules in the sample. Therefore, a non-invasive, label-free examination method for...
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-01-01
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| Series: | Nanophotonics |
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| Online Access: | https://doi.org/10.1515/nanoph-2023-0722 |
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| _version_ | 1846157299690766336 |
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| author | Guo Fangyin Lin Fangrui Shen Binglin Wang Shiqi Li Yanping Guo Jiaqing Chen Yongqiang Liu Yuqing Lu Yuan Hu Rui He Jun Liao Changrui Wang Yiping Qu Junle Liu Liwei |
| author_facet | Guo Fangyin Lin Fangrui Shen Binglin Wang Shiqi Li Yanping Guo Jiaqing Chen Yongqiang Liu Yuqing Lu Yuan Hu Rui He Jun Liao Changrui Wang Yiping Qu Junle Liu Liwei |
| author_sort | Guo Fangyin |
| collection | DOAJ |
| description | Basal cell carcinoma (BCC) is a common type of skin cancer. Conventional approaches to BCC diagnosis often involve invasive histological examinations that can distort or even destroy information derived from the biomolecules in the sample. Therefore, a non-invasive, label-free examination method for the clinical diagnosis of BCC represents a critical advance. This study combined spectral- and time-resolved two-photon microscopy with a spectral phasor to extract rich biochemical information describing macroscopic tumor morphology and microscopic tumor metabolism. The proposed optical imaging technique achieved the rapid and efficient separation of tumor structures in systematic research conducted on normal and BCC human skin tissues. The results demonstrate that a combination of multidimensional data (e.g., fluorescence intensity, spectrum, and lifetime) with a spectral phasor can accurately identify tumor boundaries and achieve rapid separation. This label-free, real-time, multidimensional imaging technique serves as a complement to the conventional tumor diagnostic toolbox and demonstrates significant potential for the early diagnosis of BCC and wider applications in intraoperative auxiliary imaging. |
| format | Article |
| id | doaj-art-4928d5821d944aed83fd55e20903b3e4 |
| institution | Kabale University |
| issn | 2192-8606 2192-8614 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanophotonics |
| spelling | doaj-art-4928d5821d944aed83fd55e20903b3e42024-11-25T11:19:11ZengDe GruyterNanophotonics2192-86062192-86142024-01-0113221722710.1515/nanoph-2023-0722Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopyGuo Fangyin0Lin Fangrui1Shen Binglin2Wang Shiqi3Li Yanping4Guo Jiaqing5Chen Yongqiang6Liu Yuqing7Lu Yuan8Hu Rui9He Jun10Liao Changrui11Wang Yiping12Qu Junle13Liu Liwei14State Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaThe Sixth Affiliated Hospital of Shenzhen University and Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaState Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen518060, ChinaBasal cell carcinoma (BCC) is a common type of skin cancer. Conventional approaches to BCC diagnosis often involve invasive histological examinations that can distort or even destroy information derived from the biomolecules in the sample. Therefore, a non-invasive, label-free examination method for the clinical diagnosis of BCC represents a critical advance. This study combined spectral- and time-resolved two-photon microscopy with a spectral phasor to extract rich biochemical information describing macroscopic tumor morphology and microscopic tumor metabolism. The proposed optical imaging technique achieved the rapid and efficient separation of tumor structures in systematic research conducted on normal and BCC human skin tissues. The results demonstrate that a combination of multidimensional data (e.g., fluorescence intensity, spectrum, and lifetime) with a spectral phasor can accurately identify tumor boundaries and achieve rapid separation. This label-free, real-time, multidimensional imaging technique serves as a complement to the conventional tumor diagnostic toolbox and demonstrates significant potential for the early diagnosis of BCC and wider applications in intraoperative auxiliary imaging.https://doi.org/10.1515/nanoph-2023-0722tumor diagnosisnon-invasivelabel-free examinationoptical imagingfluorescence lifetimespectral phasor |
| spellingShingle | Guo Fangyin Lin Fangrui Shen Binglin Wang Shiqi Li Yanping Guo Jiaqing Chen Yongqiang Liu Yuqing Lu Yuan Hu Rui He Jun Liao Changrui Wang Yiping Qu Junle Liu Liwei Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy Nanophotonics tumor diagnosis non-invasive label-free examination optical imaging fluorescence lifetime spectral phasor |
| title | Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy |
| title_full | Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy |
| title_fullStr | Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy |
| title_full_unstemmed | Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy |
| title_short | Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy |
| title_sort | multidimensional quantitative characterization of basal cell carcinoma by spectral and time resolved two photon microscopy |
| topic | tumor diagnosis non-invasive label-free examination optical imaging fluorescence lifetime spectral phasor |
| url | https://doi.org/10.1515/nanoph-2023-0722 |
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