Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigation
IntroductionGastrodiae Rhizoma (referred to Tianma in Chinese), the dried tuber of Gastrodia elata Bl. (Orchidaceae), is utilized as a medicine-food homology product. Sulfur fumigation is commonly employed in the processing of Gastrodiae Rhizoma (GR). Polysaccharides are a crucial active substance p...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2024.1477689/full |
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| author | Chunyan Dai Chunyan Dai Chunyan Dai Dan Zhao Wenping Zhang Wenping Zhang Lanping Guo Chuanzhi Kang Zhuowen Chen Zhuowen Chen Xiuming Cui Xiuming Cui Tao Zhou Chengxiao Wang Chengxiao Wang Tingting Xu Tingting Xu Tingting Xu Ye Yang Ye Yang |
| author_facet | Chunyan Dai Chunyan Dai Chunyan Dai Dan Zhao Wenping Zhang Wenping Zhang Lanping Guo Chuanzhi Kang Zhuowen Chen Zhuowen Chen Xiuming Cui Xiuming Cui Tao Zhou Chengxiao Wang Chengxiao Wang Tingting Xu Tingting Xu Tingting Xu Ye Yang Ye Yang |
| author_sort | Chunyan Dai |
| collection | DOAJ |
| description | IntroductionGastrodiae Rhizoma (referred to Tianma in Chinese), the dried tuber of Gastrodia elata Bl. (Orchidaceae), is utilized as a medicine-food homology product. Sulfur fumigation is commonly employed in the processing of Gastrodiae Rhizoma (GR). Polysaccharides are a crucial active substance produced in GR, yet the impacts of sulfur fumigation on them remain unelucidated.MethodsThis study aimed to optimize the hot water extraction conditions of polysaccharides from sulfur-fumigated GR (SGCPs) and nonsulfur-fumigated GR (NGCPs). The research explored the effects of sulfur fumigation on the structure and antioxidant activity of GR polysaccharides.Results and discussionThe results showed that the optimal extraction conditions for SGCPs and NGCPs were 67°C for 31 min with a liquid-to-material ratio of 15 mL/g and 64°C for 32 min with a liquid-to-material ratio of 17 mL/g, respectively. Compared with NGCPs, SGCPs exhibited significantly reduced DPPH radical, hydroxyl radical, ABTS+ radical scavenging activity, and Fe2+ chelating ability. Moreover, both NGCPs and SGCPs offered significant protective effects against H2O2−induced oxidative damage in RAW264.7 cells, but the protective effect of SGCPs was significantly lower than that of NGCPs. NMR analyses revealed that the main chain connections of SGCP3 and NGCP3 were both →4)-α-D-Glcp-(1→, and sulfur fumigation increased the number of repeating unit structures →4)-D-Glcp-(1 → in GR polysaccharides. SGCP3 and NGCP3 had the same monosaccharides composition but different molar ratios, with molecular weights of 727,650 and 39,991 Da, respectively. In general, sulfur fumigation reduced the antioxidant activities of GR polysaccharides by altering their structure and composition. |
| format | Article |
| id | doaj-art-48a9b1140bff4362a1d41f8dc84b0de3 |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-48a9b1140bff4362a1d41f8dc84b0de32024-12-04T15:00:50ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2024-12-011110.3389/fnut.2024.14776891477689Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigationChunyan Dai0Chunyan Dai1Chunyan Dai2Dan Zhao3Wenping Zhang4Wenping Zhang5Lanping Guo6Chuanzhi Kang7Zhuowen Chen8Zhuowen Chen9Xiuming Cui10Xiuming Cui11Tao Zhou12Chengxiao Wang13Chengxiao Wang14Tingting Xu15Tingting Xu16Tingting Xu17Ye Yang18Ye Yang19School of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, ChinaPostdoctoral Fellow, Mobile Station of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, ChinaGuizhou University of Traditional Chinese Medicine, Guiyang, ChinaSchool of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, ChinaChinese Medica Resources Center, China Academy of Chinese Medicinal Sciences, Beijing, ChinaChinese Medica Resources Center, China Academy of Chinese Medicinal Sciences, Beijing, ChinaSchool of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, ChinaSchool of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, ChinaGuizhou University of Traditional Chinese Medicine, Guiyang, ChinaSchool of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, ChinaSchool of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, ChinaKunming Medical University Haiyuan College, Kunming, ChinaSchool of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, ChinaIntroductionGastrodiae Rhizoma (referred to Tianma in Chinese), the dried tuber of Gastrodia elata Bl. (Orchidaceae), is utilized as a medicine-food homology product. Sulfur fumigation is commonly employed in the processing of Gastrodiae Rhizoma (GR). Polysaccharides are a crucial active substance produced in GR, yet the impacts of sulfur fumigation on them remain unelucidated.MethodsThis study aimed to optimize the hot water extraction conditions of polysaccharides from sulfur-fumigated GR (SGCPs) and nonsulfur-fumigated GR (NGCPs). The research explored the effects of sulfur fumigation on the structure and antioxidant activity of GR polysaccharides.Results and discussionThe results showed that the optimal extraction conditions for SGCPs and NGCPs were 67°C for 31 min with a liquid-to-material ratio of 15 mL/g and 64°C for 32 min with a liquid-to-material ratio of 17 mL/g, respectively. Compared with NGCPs, SGCPs exhibited significantly reduced DPPH radical, hydroxyl radical, ABTS+ radical scavenging activity, and Fe2+ chelating ability. Moreover, both NGCPs and SGCPs offered significant protective effects against H2O2−induced oxidative damage in RAW264.7 cells, but the protective effect of SGCPs was significantly lower than that of NGCPs. NMR analyses revealed that the main chain connections of SGCP3 and NGCP3 were both →4)-α-D-Glcp-(1→, and sulfur fumigation increased the number of repeating unit structures →4)-D-Glcp-(1 → in GR polysaccharides. SGCP3 and NGCP3 had the same monosaccharides composition but different molar ratios, with molecular weights of 727,650 and 39,991 Da, respectively. In general, sulfur fumigation reduced the antioxidant activities of GR polysaccharides by altering their structure and composition.https://www.frontiersin.org/articles/10.3389/fnut.2024.1477689/fullGastrodiae RhizomaGastrodia elata polysaccharidessulfur fumigationstructural characterizationantioxidant activityglycosidic bond |
| spellingShingle | Chunyan Dai Chunyan Dai Chunyan Dai Dan Zhao Wenping Zhang Wenping Zhang Lanping Guo Chuanzhi Kang Zhuowen Chen Zhuowen Chen Xiuming Cui Xiuming Cui Tao Zhou Chengxiao Wang Chengxiao Wang Tingting Xu Tingting Xu Tingting Xu Ye Yang Ye Yang Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigation Frontiers in Nutrition Gastrodiae Rhizoma Gastrodia elata polysaccharides sulfur fumigation structural characterization antioxidant activity glycosidic bond |
| title | Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigation |
| title_full | Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigation |
| title_fullStr | Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigation |
| title_full_unstemmed | Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigation |
| title_short | Comparative analysis of antioxidant activity and structural changes of Gastrodiae Rhizoma polysaccharides between sulfur-fumigation and nonsulfur-fumigation |
| title_sort | comparative analysis of antioxidant activity and structural changes of gastrodiae rhizoma polysaccharides between sulfur fumigation and nonsulfur fumigation |
| topic | Gastrodiae Rhizoma Gastrodia elata polysaccharides sulfur fumigation structural characterization antioxidant activity glycosidic bond |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2024.1477689/full |
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