Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus
Lateral flow immunoassays (LFIAs) are easy‐to‐use antigen tests that provide different signal readouts, with colorimetric readouts being the most commonly used. However, these analytical devices have relatively low sensitivity and produce semiquantitative results, limiting their diagnostic applicati...
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| Format: | Article |
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Wiley-VCH
2024-11-01
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| Series: | Small Science |
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| Online Access: | https://doi.org/10.1002/smsc.202400259 |
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| author | Lara González‐Cabaleiro Carlos Fernández‐Lodeiro Lorena Vázquez‐Iglesias Pablo Soriano‐Maldonado Mark J. van Raaij Gustavo Bodelón Jorge Pérez‐Juste Isabel Pastoriza‐Santos |
| author_facet | Lara González‐Cabaleiro Carlos Fernández‐Lodeiro Lorena Vázquez‐Iglesias Pablo Soriano‐Maldonado Mark J. van Raaij Gustavo Bodelón Jorge Pérez‐Juste Isabel Pastoriza‐Santos |
| author_sort | Lara González‐Cabaleiro |
| collection | DOAJ |
| description | Lateral flow immunoassays (LFIAs) are easy‐to‐use antigen tests that provide different signal readouts, with colorimetric readouts being the most commonly used. However, these analytical devices have relatively low sensitivity and produce semiquantitative results, limiting their diagnostic applications. Herein, we address these challenges by implementing a digital surface‐enhanced Raman spectroscopy (SERS)‐based LFIA for the accurate and ultrasensitive quantitative detection of SARS‐CoV‐2 nucleocapsid (N) protein. Compared with average SERS intensity measurements, the digital approach allowed to overcome fluctuations in Raman scattering signals, thereby increasing the analytical sensitivity of the assay. Our method exhibited a quantification range of the viral protein in nasal swabs from 0.001 to 10 pg mL−1, and a limit of detection down to 1.9 aM (0.9 fg mL−1), improving colorimetric LFIAs and conventional‐SERS‐based LFIAs by several orders of magnitude. Importantly, this approach shows an analytical sensitivity of 0.03 TCID50 mL−1, which is greater than that reported by other immunoassays. In conclusion, we successfully demonstrate the robust detection and quantification of SARS‐CoV‐2N protein in nasal swabs at ultralow concentrations. The improvement in the sensitivity of LFIA by digital SERS may pave the way to translate this technology into the diagnostic arena for the ultrasensitive detection of microbes and disease biomarkers. |
| format | Article |
| id | doaj-art-489cfb48c91a4632957cf307abe3731c |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Science |
| spelling | doaj-art-489cfb48c91a4632957cf307abe3731c2024-11-11T15:33:38ZengWiley-VCHSmall Science2688-40462024-11-01411n/an/a10.1002/smsc.202400259Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 VirusLara González‐Cabaleiro0Carlos Fernández‐Lodeiro1Lorena Vázquez‐Iglesias2Pablo Soriano‐Maldonado3Mark J. van Raaij4Gustavo Bodelón5Jorge Pérez‐Juste6Isabel Pastoriza‐Santos7CINBIO Universidade de Vigo, Campus Universitario As Lagoas Marcosende Vigo 36310 SpainCINBIO Universidade de Vigo, Campus Universitario As Lagoas Marcosende Vigo 36310 SpainCINBIO Universidade de Vigo, Campus Universitario As Lagoas Marcosende Vigo 36310 SpainCentro Nacional de Biotecnología (CNB‐CSIC) calle Darwin 3 Madrid 28049 SpainCentro Nacional de Biotecnología (CNB‐CSIC) calle Darwin 3 Madrid 28049 SpainCINBIO Universidade de Vigo, Campus Universitario As Lagoas Marcosende Vigo 36310 SpainCINBIO Universidade de Vigo, Campus Universitario As Lagoas Marcosende Vigo 36310 SpainCINBIO Universidade de Vigo, Campus Universitario As Lagoas Marcosende Vigo 36310 SpainLateral flow immunoassays (LFIAs) are easy‐to‐use antigen tests that provide different signal readouts, with colorimetric readouts being the most commonly used. However, these analytical devices have relatively low sensitivity and produce semiquantitative results, limiting their diagnostic applications. Herein, we address these challenges by implementing a digital surface‐enhanced Raman spectroscopy (SERS)‐based LFIA for the accurate and ultrasensitive quantitative detection of SARS‐CoV‐2 nucleocapsid (N) protein. Compared with average SERS intensity measurements, the digital approach allowed to overcome fluctuations in Raman scattering signals, thereby increasing the analytical sensitivity of the assay. Our method exhibited a quantification range of the viral protein in nasal swabs from 0.001 to 10 pg mL−1, and a limit of detection down to 1.9 aM (0.9 fg mL−1), improving colorimetric LFIAs and conventional‐SERS‐based LFIAs by several orders of magnitude. Importantly, this approach shows an analytical sensitivity of 0.03 TCID50 mL−1, which is greater than that reported by other immunoassays. In conclusion, we successfully demonstrate the robust detection and quantification of SARS‐CoV‐2N protein in nasal swabs at ultralow concentrations. The improvement in the sensitivity of LFIA by digital SERS may pave the way to translate this technology into the diagnostic arena for the ultrasensitive detection of microbes and disease biomarkers.https://doi.org/10.1002/smsc.202400259digital SERSNanoBiosensorSARS‐CoV‐2SERS‐based LFIA |
| spellingShingle | Lara González‐Cabaleiro Carlos Fernández‐Lodeiro Lorena Vázquez‐Iglesias Pablo Soriano‐Maldonado Mark J. van Raaij Gustavo Bodelón Jorge Pérez‐Juste Isabel Pastoriza‐Santos Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus Small Science digital SERS NanoBiosensor SARS‐CoV‐2 SERS‐based LFIA |
| title | Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus |
| title_full | Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus |
| title_fullStr | Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus |
| title_full_unstemmed | Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus |
| title_short | Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS‐CoV‐2 Virus |
| title_sort | pushing the limits of lateral flow immunoassay by digital sers for the ultralow detection of sars cov 2 virus |
| topic | digital SERS NanoBiosensor SARS‐CoV‐2 SERS‐based LFIA |
| url | https://doi.org/10.1002/smsc.202400259 |
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