Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant
The objective of the present study was to evaluate the protective effects of nanoemulsion of thyme (Thymus vulgaris) essential oil (EO) during the liquid state preservation of ram semen. Storage media were prepared: one containing Thymus vulgaris essential oil (Th), another with its nanoemulsion for...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Animal Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2024.1479602/full |
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| author | Samia Khnissi Rania Maaloul Hela Chalouati Laura Mara Dorra Aouadi Basma Adouani Maria Dattena Ikram Khémiri Sami Fattouch |
| author_facet | Samia Khnissi Rania Maaloul Hela Chalouati Laura Mara Dorra Aouadi Basma Adouani Maria Dattena Ikram Khémiri Sami Fattouch |
| author_sort | Samia Khnissi |
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| description | The objective of the present study was to evaluate the protective effects of nanoemulsion of thyme (Thymus vulgaris) essential oil (EO) during the liquid state preservation of ram semen. Storage media were prepared: one containing Thymus vulgaris essential oil (Th), another with its nanoemulsion form (NTh), and a control sample without essential oil (Dovx). The quality of the semen diluted in the prepared media after preservation at 4°C and 15°C was examined by measuring the two parameters indicative of semen quality: semen motility and progressivity refer to the ability of ram sperm cells to move actively and efficiently, which is crucial for successful fertilization. The results showed a decrease in the mobility and progressivity of the sperm in all the mediums, but the thyme EO and its nanoemulsion showed slight decreases compared to the control medium (p<0.05). On the other hand, the study identified a negative impact of thyme EO nanoemulsions on catalase concentrations, potentially leading to mobility inhibition (p<0.05). In addition, the nanoemulsion significantly (p<0,05) decreased the malondialdehyde concentration, increased the total protein and the glucose contents, the lactate dehydrogenase activity. A significant decrease in calcium content was observed (p < 0.05). The essential oil of Thymus vulgaris, combined with nanotechnology encapsulation as a delivery method (nanoemulsion), demonstrated a notable and effective role in enhancing the preservation of ovine semen. |
| format | Article |
| id | doaj-art-487e41af803243d8ad56940f08014d39 |
| institution | Kabale University |
| issn | 2673-6225 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Animal Science |
| spelling | doaj-art-487e41af803243d8ad56940f08014d392025-01-03T06:47:18ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252025-01-01510.3389/fanim.2024.14796021479602Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidantSamia Khnissi0Rania Maaloul1Hela Chalouati2Laura Mara3Dorra Aouadi4Basma Adouani5Maria Dattena6Ikram Khémiri7Sami Fattouch8Laboratory of Animal and Forage Production, National Institute of Agronomic Research of Tunisia (INRAT), University of Carthage, Tunis, TunisiaLaboratory of Animal and Forage Production, National Institute of Agronomic Research of Tunisia (INRAT), University of Carthage, Tunis, TunisiaLaboratory of Animal and Forage Production, National Institute of Agronomic Research of Tunisia (INRAT), University of Carthage, Tunis, TunisiaDepartment of Animal Science, Agricultural Research Agency of Sardinia, Olmedo, ItalyIndependent Researcher, TunisiaLaboratory of Mycology, Pathology and Biomarkers, Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, TunisiaDepartment of Animal Science, Agricultural Research Agency of Sardinia, Olmedo, ItalyLaboratory of Mycology, Pathology and Biomarkers, Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, TunisiaEcoChemistry Laboratory, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Tunis, TunisiaThe objective of the present study was to evaluate the protective effects of nanoemulsion of thyme (Thymus vulgaris) essential oil (EO) during the liquid state preservation of ram semen. Storage media were prepared: one containing Thymus vulgaris essential oil (Th), another with its nanoemulsion form (NTh), and a control sample without essential oil (Dovx). The quality of the semen diluted in the prepared media after preservation at 4°C and 15°C was examined by measuring the two parameters indicative of semen quality: semen motility and progressivity refer to the ability of ram sperm cells to move actively and efficiently, which is crucial for successful fertilization. The results showed a decrease in the mobility and progressivity of the sperm in all the mediums, but the thyme EO and its nanoemulsion showed slight decreases compared to the control medium (p<0.05). On the other hand, the study identified a negative impact of thyme EO nanoemulsions on catalase concentrations, potentially leading to mobility inhibition (p<0.05). In addition, the nanoemulsion significantly (p<0,05) decreased the malondialdehyde concentration, increased the total protein and the glucose contents, the lactate dehydrogenase activity. A significant decrease in calcium content was observed (p < 0.05). The essential oil of Thymus vulgaris, combined with nanotechnology encapsulation as a delivery method (nanoemulsion), demonstrated a notable and effective role in enhancing the preservation of ovine semen.https://www.frontiersin.org/articles/10.3389/fanim.2024.1479602/fullsemencryopreservationramThymus vulgarisnanoemulsion |
| spellingShingle | Samia Khnissi Rania Maaloul Hela Chalouati Laura Mara Dorra Aouadi Basma Adouani Maria Dattena Ikram Khémiri Sami Fattouch Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant Frontiers in Animal Science semen cryopreservation ram Thymus vulgaris nanoemulsion |
| title | Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant |
| title_full | Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant |
| title_fullStr | Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant |
| title_full_unstemmed | Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant |
| title_short | Nanotechnology-assisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant |
| title_sort | nanotechnology assisted cryopreservation of ovine semen evaluation of thymus vulgaris essential oil as a natural antioxidant |
| topic | semen cryopreservation ram Thymus vulgaris nanoemulsion |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2024.1479602/full |
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