Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest
Essential oils (EOs) and nature identical compounds (NICs) express different antimicrobial activity (AAc) which can be affected by variability in composition, stability over time, and the carrier employed for their inclusion in feedstuff and products for animal treatments. The aim of the present stu...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2024.2304042 |
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| author | Costanza Spadini Mattia Iannarelli Alicia Maria Carrillo Heredero Sabrina Lucia Montanaro Nicolò Mezzasalma Marica Simoni Simone Bertini Federico Righi Clotilde Silvia Cabassi |
| author_facet | Costanza Spadini Mattia Iannarelli Alicia Maria Carrillo Heredero Sabrina Lucia Montanaro Nicolò Mezzasalma Marica Simoni Simone Bertini Federico Righi Clotilde Silvia Cabassi |
| author_sort | Costanza Spadini |
| collection | DOAJ |
| description | Essential oils (EOs) and nature identical compounds (NICs) express different antimicrobial activity (AAc) which can be affected by variability in composition, stability over time, and the carrier employed for their inclusion in feedstuff and products for animal treatments. The aim of the present study was to evaluate the stability over time of the AAc of 8 EOs and 5 NICs, alone and in combination with Tween 20, on four major bacterial livestock pathogens (E. coli, S. Typhimurium, S. aureus and MRSA). For this purpose, minimal inhibitory concentration (MIC) and checkerboard assay (CkA) tests were performed on EOs and NICs at batch first use (T0 – only MIC), and after 12 (T1) and 24 (T2) months. Several EOs showed initial MICs lower than 2% (v/v) and, except for clove bud and cinnamon EOs, reduced their efficacy over time. The best NICs’ AAc was showed at T1 by cinnamic aldehyde (against MRSA), carvacrol (against E. coli), and thymol (except against MRSA), while at T2 the MIC values decreased for most of the NICs. The CkA between EOs and Tween 20 showed different interactions (Fractional Inhibitory Concentration -FIC- index from 0.06 to 32.04) based on bacteria and time. No interactions were found between NICs and Tween 20 (FIC index from 1.0 to 3.0). The AAc of EOs and NICs changes over time. Therefore, the preservation interval of these additives is critical for the AAc of feedstuff and products intended for livestock animals. Moreover, the NICs seem more suitable for the association with Tween 20. |
| format | Article |
| id | doaj-art-485faaec3fa0480a9f9b746ee4e7a427 |
| institution | Kabale University |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj-art-485faaec3fa0480a9f9b746ee4e7a4272025-01-02T11:34:21ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2024-12-0123118919910.1080/1828051X.2024.23040422304042Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interestCostanza Spadini0Mattia Iannarelli1Alicia Maria Carrillo Heredero2Sabrina Lucia Montanaro3Nicolò Mezzasalma4Marica Simoni5Simone Bertini6Federico Righi7Clotilde Silvia Cabassi8Dipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaDipartimento di Scienze Medico-Veterinarie, Università di ParmaEssential oils (EOs) and nature identical compounds (NICs) express different antimicrobial activity (AAc) which can be affected by variability in composition, stability over time, and the carrier employed for their inclusion in feedstuff and products for animal treatments. The aim of the present study was to evaluate the stability over time of the AAc of 8 EOs and 5 NICs, alone and in combination with Tween 20, on four major bacterial livestock pathogens (E. coli, S. Typhimurium, S. aureus and MRSA). For this purpose, minimal inhibitory concentration (MIC) and checkerboard assay (CkA) tests were performed on EOs and NICs at batch first use (T0 – only MIC), and after 12 (T1) and 24 (T2) months. Several EOs showed initial MICs lower than 2% (v/v) and, except for clove bud and cinnamon EOs, reduced their efficacy over time. The best NICs’ AAc was showed at T1 by cinnamic aldehyde (against MRSA), carvacrol (against E. coli), and thymol (except against MRSA), while at T2 the MIC values decreased for most of the NICs. The CkA between EOs and Tween 20 showed different interactions (Fractional Inhibitory Concentration -FIC- index from 0.06 to 32.04) based on bacteria and time. No interactions were found between NICs and Tween 20 (FIC index from 1.0 to 3.0). The AAc of EOs and NICs changes over time. Therefore, the preservation interval of these additives is critical for the AAc of feedstuff and products intended for livestock animals. Moreover, the NICs seem more suitable for the association with Tween 20.http://dx.doi.org/10.1080/1828051X.2024.2304042essential oilsnatural identical compoundsantimicrobial activitystabilityfeed additives |
| spellingShingle | Costanza Spadini Mattia Iannarelli Alicia Maria Carrillo Heredero Sabrina Lucia Montanaro Nicolò Mezzasalma Marica Simoni Simone Bertini Federico Righi Clotilde Silvia Cabassi Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest Italian Journal of Animal Science essential oils natural identical compounds antimicrobial activity stability feed additives |
| title | Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest |
| title_full | Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest |
| title_fullStr | Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest |
| title_full_unstemmed | Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest |
| title_short | Stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with Tween 20 against reference bacterial strains of zootechnical interest |
| title_sort | stability of the antimicrobial activity of selected essential oils and nature identical compounds and their interaction with tween 20 against reference bacterial strains of zootechnical interest |
| topic | essential oils natural identical compounds antimicrobial activity stability feed additives |
| url | http://dx.doi.org/10.1080/1828051X.2024.2304042 |
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