Individual and parentage testing in horses by pcr methodology
<b>Aim:</b> The objective of this study was to develop and test a microsatellite panel for parentage analysis in horses.<p> <b>Materials and Methods:</b> A total of 189 blood samples were collected from four different horse breeds in Turkey. We selected five horse micro...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=851 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <b>Aim:</b> The objective of this study was to develop and test a
microsatellite panel for parentage analysis in horses.<p>
<b>Materials and Methods:</b> A total of 189 blood samples were
collected from four different horse breeds in Turkey. We selected
five horse microsatellite loci and used to amplify genomic
DNA by polymerase chain reaction (PCR). The resulting
PCR products were separated on polyacrylamide gels.
Allele identification was conducted based on their base-pair
size by comparing a size standard.<p>
<b>Results:</b> A total of 53 alleles was determined ranging from
9 to 13 at each locus. The observed heterozygosity (Ho) and
expected heterozygosity (He) were ranged from 0.496 to
0.880 and from 0.800 to 0.851, respectively. Polymorphism
information content (PIC) values were observed between
0.774 and 0.832. Power of exclusion (PE) at each microsatellite
locus ranged from 0.619 to 0.702, resulting in a total
PE value of 0.99060.<p>
<b>Conclusion:</b> These results indicate that this set of microsatellite
is useful for horse parentage testing in Turkey. Due to
possible high level of inbreeding in some breeds, the use of
increased number microsatellite loci will thereby be appropriate
for avoiding a false parenting and misidentification. |
|---|---|
| ISSN: | 1309-6958 2146-1953 |