EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasion
Summary: Epithelial-to-mesenchymal transitions (EMT) and unjamming transitions provide two distinct pathways for cancer cells to become invasive, but it is still unclear to what extent these pathways are connected. Here, we addressed this question by performing 3D spheroid invasion assays on epithel...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422402649X |
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| author | Anouk van der Net Zaid Rahman Ankur D. Bordoloi Iain Muntz Peter ten Dijke Pouyan E. Boukany Gijsje H. Koenderink |
| author_facet | Anouk van der Net Zaid Rahman Ankur D. Bordoloi Iain Muntz Peter ten Dijke Pouyan E. Boukany Gijsje H. Koenderink |
| author_sort | Anouk van der Net |
| collection | DOAJ |
| description | Summary: Epithelial-to-mesenchymal transitions (EMT) and unjamming transitions provide two distinct pathways for cancer cells to become invasive, but it is still unclear to what extent these pathways are connected. Here, we addressed this question by performing 3D spheroid invasion assays on epithelial-like (A549) and mesenchymal-like (MV3) cancer cell lines in collagen-based hydrogels, where we varied both the invasive character of the cells and matrix porosity. We found that the onset time of invasion was correlated with the matrix porosity and vimentin levels, while the spheroid expansion rate correlated with MMP1 levels. Spheroids displayed solid-like (non-invasive) states in small-pore hydrogels and fluid-like (strand-based) or gas-like (disseminating cells) states in large-pore hydrogels or for mesenchymal-like cells. Our findings are consistent with different unjamming states as a function of cell motility and matrix confinement predicted in recent models for cancer invasion, but show that cell motility and matrix confinement are coupled via EMT-related matrix degradation. |
| format | Article |
| id | doaj-art-484aae2e6b1d490a82a5331aa6ff987d |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-484aae2e6b1d490a82a5331aa6ff987d2024-12-22T05:29:19ZengElsevieriScience2589-00422024-12-012712111424EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasionAnouk van der Net0Zaid Rahman1Ankur D. Bordoloi2Iain Muntz3Peter ten Dijke4Pouyan E. Boukany5Gijsje H. Koenderink6Delft University of Technology, Department of Bionanoscience, Kavli Institute of Nanoscience, Delft 2629 HZ, the NetherlandsDelft University of Technology, Department of Chemical Engineering, Delft 2629 HZ, the NetherlandsDelft University of Technology, Department of Chemical Engineering, Delft 2629 HZ, the NetherlandsDelft University of Technology, Department of Bionanoscience, Kavli Institute of Nanoscience, Delft 2629 HZ, the NetherlandsLeiden University Medical Center, Department of Cell and Chemical Biology and Oncode Institute, Leiden 2333 ZC, the NetherlandsDelft University of Technology, Department of Chemical Engineering, Delft 2629 HZ, the Netherlands; Corresponding authorDelft University of Technology, Department of Bionanoscience, Kavli Institute of Nanoscience, Delft 2629 HZ, the Netherlands; Corresponding authorSummary: Epithelial-to-mesenchymal transitions (EMT) and unjamming transitions provide two distinct pathways for cancer cells to become invasive, but it is still unclear to what extent these pathways are connected. Here, we addressed this question by performing 3D spheroid invasion assays on epithelial-like (A549) and mesenchymal-like (MV3) cancer cell lines in collagen-based hydrogels, where we varied both the invasive character of the cells and matrix porosity. We found that the onset time of invasion was correlated with the matrix porosity and vimentin levels, while the spheroid expansion rate correlated with MMP1 levels. Spheroids displayed solid-like (non-invasive) states in small-pore hydrogels and fluid-like (strand-based) or gas-like (disseminating cells) states in large-pore hydrogels or for mesenchymal-like cells. Our findings are consistent with different unjamming states as a function of cell motility and matrix confinement predicted in recent models for cancer invasion, but show that cell motility and matrix confinement are coupled via EMT-related matrix degradation.http://www.sciencedirect.com/science/article/pii/S258900422402649XBiophysicsBiomechanicsCancer |
| spellingShingle | Anouk van der Net Zaid Rahman Ankur D. Bordoloi Iain Muntz Peter ten Dijke Pouyan E. Boukany Gijsje H. Koenderink EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasion iScience Biophysics Biomechanics Cancer |
| title | EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasion |
| title_full | EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasion |
| title_fullStr | EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasion |
| title_full_unstemmed | EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasion |
| title_short | EMT-related cell-matrix interactions are linked to states of cell unjamming in cancer spheroid invasion |
| title_sort | emt related cell matrix interactions are linked to states of cell unjamming in cancer spheroid invasion |
| topic | Biophysics Biomechanics Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S258900422402649X |
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