Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packaging
Background: Foil, cellophane, and disposable plastic containers are commonly employed in food packaging, namely as coatings on food items. Nevertheless, the existence of microbes on the surface and inside the internal composition of these materials gives rise to concerns, as they have the capacity...
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2024-11-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
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| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1196 |
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| author | Mojtaba Mohammadzadeh Vazifeh Erfan Aghajani Negin Shokrollahzadeh Helia Rahmati Nakisa Sohrabi Haghdoost |
| author_facet | Mojtaba Mohammadzadeh Vazifeh Erfan Aghajani Negin Shokrollahzadeh Helia Rahmati Nakisa Sohrabi Haghdoost |
| author_sort | Mojtaba Mohammadzadeh Vazifeh |
| collection | DOAJ |
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Background: Foil, cellophane, and disposable plastic containers are commonly employed in food packaging, namely as coatings on food items. Nevertheless, the existence of microbes on the surface and inside the internal composition of these materials gives rise to concerns, as they have the capacity to multiply under ideal circumstances and spread to food items, thereby jeopardizing the safety and quality of the food.
Objective: This study aimed at examining the number of microorganisms and identifying specific bacterial species within frequently used food packaging materials like foil, cellophane, and disposable plastic containers.
Methods: samples of foil, cellophane, and disposable plastic containers were collected from various popular brands in Iran. The overall bacterial count wضas determined using defibering, flooding, and smear techniques followed by culturing on Tryptone Glucose Extract Agar (TGEA) medium. To identify the bacterial species, various biochemical tests were performed, including fermentation, motility testing, catalase test, oxidase test, and methyl red-Voges Proskauer (MR-VP) test.
Results: Bacterial quantity ranged from 0 CFU/g to 8.3 × 103 CFU/g in analyzed samples. All the samples had primary contaminants mainly Bacillaceae family bacteria that could produce spores. Disposable plastic containers had the lowest bacterial count whereas cellophane showed the highest bacterial contamination level. Among Bacillaceae family members Bacillus licheniformis was dominant.
Conclusion: The study's findings emphasize the possibility of microbial contamination in food packaging materials, including cellophane. These results indicate that the food packaging industry should adopt a rigorous approach to hazard evaluation and Important Control Points (HACCP) in order to guarantee the microbiological safety and efficacy of packaging materials.
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| format | Article |
| id | doaj-art-48308758b5034e2b98816bd6f38c0f3d |
| institution | Kabale University |
| issn | 1815-0993 2959-183X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj-art-48308758b5034e2b98816bd6f38c0f3d2024-11-10T12:08:05ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2024-11-0124410.32947/ajps.v24i4.1196Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packagingMojtaba Mohammadzadeh Vazifeh0Erfan Aghajani1Negin Shokrollahzadeh2Helia Rahmati3Nakisa Sohrabi Haghdoost4Department of Microbial Biotechnology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, IranDepartment of Microbial Biotechnology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, IranDepartment of Microbial Biotechnology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, IranArman Hedayat Noush Alborz Food Research Laboratory, Tehran, IranDepartment of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran Background: Foil, cellophane, and disposable plastic containers are commonly employed in food packaging, namely as coatings on food items. Nevertheless, the existence of microbes on the surface and inside the internal composition of these materials gives rise to concerns, as they have the capacity to multiply under ideal circumstances and spread to food items, thereby jeopardizing the safety and quality of the food. Objective: This study aimed at examining the number of microorganisms and identifying specific bacterial species within frequently used food packaging materials like foil, cellophane, and disposable plastic containers. Methods: samples of foil, cellophane, and disposable plastic containers were collected from various popular brands in Iran. The overall bacterial count wضas determined using defibering, flooding, and smear techniques followed by culturing on Tryptone Glucose Extract Agar (TGEA) medium. To identify the bacterial species, various biochemical tests were performed, including fermentation, motility testing, catalase test, oxidase test, and methyl red-Voges Proskauer (MR-VP) test. Results: Bacterial quantity ranged from 0 CFU/g to 8.3 × 103 CFU/g in analyzed samples. All the samples had primary contaminants mainly Bacillaceae family bacteria that could produce spores. Disposable plastic containers had the lowest bacterial count whereas cellophane showed the highest bacterial contamination level. Among Bacillaceae family members Bacillus licheniformis was dominant. Conclusion: The study's findings emphasize the possibility of microbial contamination in food packaging materials, including cellophane. These results indicate that the food packaging industry should adopt a rigorous approach to hazard evaluation and Important Control Points (HACCP) in order to guarantee the microbiological safety and efficacy of packaging materials. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1196FoilCellophaneDisposable plastic containerFood packagingFamily Bacillaceae |
| spellingShingle | Mojtaba Mohammadzadeh Vazifeh Erfan Aghajani Negin Shokrollahzadeh Helia Rahmati Nakisa Sohrabi Haghdoost Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packaging Al-Mustansiriyah Journal of Pharmaceutical Sciences Foil Cellophane Disposable plastic container Food packaging Family Bacillaceae |
| title | Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packaging |
| title_full | Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packaging |
| title_fullStr | Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packaging |
| title_full_unstemmed | Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packaging |
| title_short | Prevalence and identification of Bacillus species in cellophane, foil, and disposable plastic containers used for food packaging |
| title_sort | prevalence and identification of bacillus species in cellophane foil and disposable plastic containers used for food packaging |
| topic | Foil Cellophane Disposable plastic container Food packaging Family Bacillaceae |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1196 |
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