Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infection
Common carp is a globally farmed and economically important freshwater fish species. Due to the intensive culture conditions, the farmed common carp are susceptible to various pathogens, causing high mortality and serious economic losses to the carp culture industry. The route of infection is usuall...
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Elsevier
2024-12-01
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| Series: | Comparative Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S295031162400034X |
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| author | Jiali Wang Qi Zhou Yanliang Jiang |
| author_facet | Jiali Wang Qi Zhou Yanliang Jiang |
| author_sort | Jiali Wang |
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| description | Common carp is a globally farmed and economically important freshwater fish species. Due to the intensive culture conditions, the farmed common carp are susceptible to various pathogens, causing high mortality and serious economic losses to the carp culture industry. The route of infection is usually through the main mucosal tissues. In order to generate an effective strategy to better manage the fish disease, it is important to understand mucosal immune related genes as well as their expressions during infection. In this study, the common carp mucin gene family were identified and characterized through bioinformatics analysis, and their expression patterns were examined in healthy tissues of common carp as well as the mucosal tissues after the infection of Aeromonas hydrophila. Our results showed that, there were a set of 11 mucin genes across the entire common carp genome. Functional domain prediction and phylogenetic analysis supported their annotation and orthologies. Examining gene copy number across several vertebrates showed that muc4 and muc6 genes were present in other vertebrate, but were lost in teleost fish, which appeared as a result of gene loss after the split of teleost fish from tetrapods during evolution. The mucin genes presented tissue-specific expression patterns in various healthy common carp tissues, with relatively high expression levels in mucosal tissues, indicating their important roles likely in mucosal immune response. Furthermore, the expression of all mucin genes except for muc2-1 were significantly regulated at one or more timepoints in minimum one mucosal tissue after bacterial infection, suggesting that the mucin gene family played critical roles in the mucosal immunity of common carp in response to pathogenic invasion. Collectively, our findings provided fundamental genomic resources for better understanding the vital roles of mucin genes in the defense against pathogen invasion in teleost. |
| format | Article |
| id | doaj-art-47d1cc5fe4bb4b2aa180fd4a9b6de9f5 |
| institution | Kabale University |
| issn | 2950-3116 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Comparative Immunology Reports |
| spelling | doaj-art-47d1cc5fe4bb4b2aa180fd4a9b6de9f52024-12-18T08:56:03ZengElsevierComparative Immunology Reports2950-31162024-12-017200167Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infectionJiali Wang0Qi Zhou1Yanliang Jiang2Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, CAFS Key Laboratory of Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, China; College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, CAFS Key Laboratory of Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, China; College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, CAFS Key Laboratory of Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, China; Corresponding author.Common carp is a globally farmed and economically important freshwater fish species. Due to the intensive culture conditions, the farmed common carp are susceptible to various pathogens, causing high mortality and serious economic losses to the carp culture industry. The route of infection is usually through the main mucosal tissues. In order to generate an effective strategy to better manage the fish disease, it is important to understand mucosal immune related genes as well as their expressions during infection. In this study, the common carp mucin gene family were identified and characterized through bioinformatics analysis, and their expression patterns were examined in healthy tissues of common carp as well as the mucosal tissues after the infection of Aeromonas hydrophila. Our results showed that, there were a set of 11 mucin genes across the entire common carp genome. Functional domain prediction and phylogenetic analysis supported their annotation and orthologies. Examining gene copy number across several vertebrates showed that muc4 and muc6 genes were present in other vertebrate, but were lost in teleost fish, which appeared as a result of gene loss after the split of teleost fish from tetrapods during evolution. The mucin genes presented tissue-specific expression patterns in various healthy common carp tissues, with relatively high expression levels in mucosal tissues, indicating their important roles likely in mucosal immune response. Furthermore, the expression of all mucin genes except for muc2-1 were significantly regulated at one or more timepoints in minimum one mucosal tissue after bacterial infection, suggesting that the mucin gene family played critical roles in the mucosal immunity of common carp in response to pathogenic invasion. Collectively, our findings provided fundamental genomic resources for better understanding the vital roles of mucin genes in the defense against pathogen invasion in teleost.http://www.sciencedirect.com/science/article/pii/S295031162400034XCommon carpCyprinus carpioPhylogenetic analysisMucinMucosal immune response |
| spellingShingle | Jiali Wang Qi Zhou Yanliang Jiang Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infection Comparative Immunology Reports Common carp Cyprinus carpio Phylogenetic analysis Mucin Mucosal immune response |
| title | Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infection |
| title_full | Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infection |
| title_fullStr | Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infection |
| title_full_unstemmed | Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infection |
| title_short | Genome-wide analysis of common carp (Cyprinus carpio) mucin genes and their roles in mucosal immune response following the Aeromonas hydrophila infection |
| title_sort | genome wide analysis of common carp cyprinus carpio mucin genes and their roles in mucosal immune response following the aeromonas hydrophila infection |
| topic | Common carp Cyprinus carpio Phylogenetic analysis Mucin Mucosal immune response |
| url | http://www.sciencedirect.com/science/article/pii/S295031162400034X |
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