Toxic Effects of Lead Exposure on Freshwater Climbing Perch, <i>Anabas testudineus</i>, and Bioremediation Using <i>Ocimum sanctum</i> Leaf Powder

The acute and chronic toxicity of lead to <i>Anabas testudineus</i> was determined in this study using static replacement bioassay testing. During the chronic toxicity studies, an experiment on the bioremediation of lead toxicity using <i>Ocimum sanctum</i> leaf powder was co...

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Bibliographic Details
Main Authors: Nimai Chandra Saha, Arnab Chatterjee, Priyajit Banerjee, Ritwick Bhattacharya, Auroshree Sadhu, Paolo Pastorino, Shubhajit Saha
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Toxics
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Online Access:https://www.mdpi.com/2305-6304/12/12/927
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Summary:The acute and chronic toxicity of lead to <i>Anabas testudineus</i> was determined in this study using static replacement bioassay testing. During the chronic toxicity studies, an experiment on the bioremediation of lead toxicity using <i>Ocimum sanctum</i> leaf powder was conducted. The 96 h LC<sub>50</sub> values of lead for <i>Anabas testudineus</i> was 1.08 mg/L. Different biomarkers, such as the hepatosomatic index, gonadosomatic index, and fecundity, were significantly lower in fish subjected to 10% and 20% of the 96 h LC<sub>50</sub> values of lead, compared to controls. The 45-day chronic exposure of fish to lead concentrations of 0.2 mg/L and above significantly lowered the number of total RBC, hemoglobin content, HCT (%), plasma protein, and cholesterol while decreasing the level of total WBC, plasma glucose, creatinine, serum AST and serum ALT. The leaf powder of <i>Ocimum sanctum</i> plays a significant role in ameliorating lead toxicity.
ISSN:2305-6304