Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility
Arylalkylamine-N-acetyl-transferase (AA-NAT) is one of several genes that influence sheep reproduction. Thus, the objective of this study was to investigate whether genetic variability within the AA-NAT gene influenced the reproductive performance of Awassi and Hamdani ewes. A total of 99 twin and 1...
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| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2024.2352771 |
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| author | Waleed A. Abd Al-Jabar Tahreer M. Al-Thuwaini |
| author_facet | Waleed A. Abd Al-Jabar Tahreer M. Al-Thuwaini |
| author_sort | Waleed A. Abd Al-Jabar |
| collection | DOAJ |
| description | Arylalkylamine-N-acetyl-transferase (AA-NAT) is one of several genes that influence sheep reproduction. Thus, the objective of this study was to investigate whether genetic variability within the AA-NAT gene influenced the reproductive performance of Awassi and Hamdani ewes. A total of 99 twin and 101 single-progeny ewes were analyzed for genomic DNA. Polymerase chain reaction (PCR) was used to produce amplicons of 300, 313, and 287 bp from exons 1, 2, and 3 of the AA-NAT gene. A 300-bp amplicon was genotyped, resulting in two genotypes: GG and GA. Through sequence analysis, a mutation 203 G > A was identified in the GA genotype. The statistical analysis revealed a strong correlation between the single nucleotide polymorphism (SNP) 203 G > A and reproductive performance. Ewes carrying this mutation showed significantly increased litter sizes, twinning rates, lambing rates, and fewer days to lambing compared to those carrying GG. These findings demonstrate that the presence of the 203 G > A SNP variant has a significant positive impact on litter sizes and enhances the fertility of Awassi and Hamdani sheep. |
| format | Article |
| id | doaj-art-47be1077e4cd401a8d949f96f08c70a8 |
| institution | Kabale University |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-47be1077e4cd401a8d949f96f08c70a82024-12-31T18:28:12ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2352771Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertilityWaleed A. Abd Al-Jabar0Tahreer M. Al-Thuwaini1Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, IraqArylalkylamine-N-acetyl-transferase (AA-NAT) is one of several genes that influence sheep reproduction. Thus, the objective of this study was to investigate whether genetic variability within the AA-NAT gene influenced the reproductive performance of Awassi and Hamdani ewes. A total of 99 twin and 101 single-progeny ewes were analyzed for genomic DNA. Polymerase chain reaction (PCR) was used to produce amplicons of 300, 313, and 287 bp from exons 1, 2, and 3 of the AA-NAT gene. A 300-bp amplicon was genotyped, resulting in two genotypes: GG and GA. Through sequence analysis, a mutation 203 G > A was identified in the GA genotype. The statistical analysis revealed a strong correlation between the single nucleotide polymorphism (SNP) 203 G > A and reproductive performance. Ewes carrying this mutation showed significantly increased litter sizes, twinning rates, lambing rates, and fewer days to lambing compared to those carrying GG. These findings demonstrate that the presence of the 203 G > A SNP variant has a significant positive impact on litter sizes and enhances the fertility of Awassi and Hamdani sheep.https://www.tandfonline.com/doi/10.1080/10495398.2024.2352771Fertilitypolymorphismseasonaltwinning ratesheep |
| spellingShingle | Waleed A. Abd Al-Jabar Tahreer M. Al-Thuwaini Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility Animal Biotechnology Fertility polymorphism seasonal twinning rate sheep |
| title | Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility |
| title_full | Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility |
| title_fullStr | Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility |
| title_full_unstemmed | Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility |
| title_short | Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility |
| title_sort | variation in the aa nat gene g203a is associated with awassi and hamdani sheep fertility |
| topic | Fertility polymorphism seasonal twinning rate sheep |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2024.2352771 |
| work_keys_str_mv | AT waleedaabdaljabar variationintheaanatgeneg203aisassociatedwithawassiandhamdanisheepfertility AT tahreermalthuwaini variationintheaanatgeneg203aisassociatedwithawassiandhamdanisheepfertility |