Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer

Nasopharyngeal carcinoma (NPC) is a rare type of tumor originating from the region behind the nasal cavity and above the throat. The primary causes of NPC include Epstein-Barr virus infection, genetic mutations, and environmental factors, such as lifestyle. Global and Vietnamese studies have indicat...

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Main Authors:	Lao Đức Thuận, Nguyễn Thị Ngọc Thảo, Thiều Hồng Huệ, Nguyễn Trung Hiếu, Phạm Thị Thúy Ngọc, Trần Bích Thư, Lê Huyền Ái Thúy
Format:	Article
Language:	English
Published:	HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE 2024-04-01
Series:	Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ
Subjects:	phân tích tổng hợp p16ink4α (cyclin dependent kinase inhibitor 2a) ung thư vòm họng việt nam
Online Access:	https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/3040
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author	Lao Đức Thuận Nguyễn Thị Ngọc Thảo Thiều Hồng Huệ Nguyễn Trung Hiếu Phạm Thị Thúy Ngọc Trần Bích Thư Lê Huyền Ái Thúy
author_facet	Lao Đức Thuận Nguyễn Thị Ngọc Thảo Thiều Hồng Huệ Nguyễn Trung Hiếu Phạm Thị Thúy Ngọc Trần Bích Thư Lê Huyền Ái Thúy
author_sort	Lao Đức Thuận
collection	DOAJ
description	Nasopharyngeal carcinoma (NPC) is a rare type of tumor originating from the region behind the nasal cavity and above the throat. The primary causes of NPC include Epstein-Barr virus infection, genetic mutations, and environmental factors, such as lifestyle. Global and Vietnamese studies have indicated enhanced methylation in the promoter region of CpG islands, contributing to the development of various cancers, including NPC (Chan, Teo, & Johnson, 2002). The p16INK4α gene, also known as cyclin-dependent kinase inhibitor, is a tumor suppressor gene that inhibits Cyclin-Dependent Kinase (CDK), leading to the inhibition of CDK interaction with cyclin, thereby inhibiting the phosphorylation process on pRb, halting the cell cycle. The p16INK4α gene is located on the short arm of chromosome 9 at position 9p21.3, consisting of three exons encoding 156 amino acids (Coppé et al., 2011). According to a meta-analysis of 11 papers on methylation processes on the p16 INK4α gene conducted by Xiao et al. in 2016, significantly higher levels of methylation were found in NPC tissue samples compared to non-tumor samples (Xiao et al., 2016). Additionally, methylation on the p16INK4α gene in blood samples from NPC patients was also significantly higher compared to normal blood samples. Therefore, this article aims to provide further scientific evidence on the enhanced methylation status in the promoter region of CpG islands of NPC patients based on the analysis of the p16INK4α gene using statistical synthesis methods. The research results demonstrated an uneven expression frequency of the p16INK4α gene between the two patient groups and the control group. In the patient group, we observed a Q value of 104.5223 with degrees of freedom (DF) = 13 and a p-value < 0.0001, along with an I2 index of 87.56%, with a 95% confidence interval for I2 from 80.84 to 91.9. Meanwhile, in the control group, the Q value was 56.1888 (p < 0.001), and the I2 index reached 76.86%, with a 95% confidence interval for I2 from 61.38 to 86.14. The weighted frequency of the patient and control groups was 63.478% and 7.966%, respectively, indicating a significant difference between the patient and control groups, with a high Q value and a very low p-value. Other factors that may influence the methylation process, such as ethnicity and sample source, were also included in the analysis. The results showed a significant correlation between the methylation process in these two groups and the development of NPC. Specifically, the Odds Ratios (OR) for China and Africa were 7.002 and 6.47, respectively. We also observed that the OR for samples from the mucosa and body fluids were 6.802 and 5.516, respectively. The analysis confirmed that the expression of the p16INK4α gene is strongly related to the formation of throat cancer. Therefore, this data provides a foundation for experimental studies on NPC samples collected from the Vietnamese population.
format	Article
id	doaj-art-47b5eb4a70a949769ad4d4fa72ce090a
institution	Kabale University
issn	2734-9322 2734-9594
language	English
publishDate	2024-04-01
publisher	HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
record_format	Article
series	Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ
spelling	doaj-art-47b5eb4a70a949769ad4d4fa72ce090a2025-01-11T06:52:59ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCETạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ2734-93222734-95942024-04-0119231210.46223/HCMCOUJS.tech.vi.19.2.3040.20242142Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancerLao Đức Thuận0Nguyễn Thị Ngọc Thảo1Thiều Hồng Huệ2Nguyễn Trung Hiếu3Phạm Thị Thúy Ngọc4Trần Bích Thư5Lê Huyền Ái Thúy6Trường Đại Học Mở Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí MinhTrường Đại Học Mở Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí Minh Viện Pasteur Thành Phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrường Đại học Mở Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí MinhTrường Đại Học Mở Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí Minh Viện Pasteur Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí MinhTrường Đại Học Mở Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí Minh Viện Pasteur Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí MinhTrường Đại Học Khoa Học Tự Nhiên, Thành Phố Hồ Chí Minh Đại học Quốc Gia Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí MinhTrường Đại Học Mở Thành Phố Hồ Chí Minh, Thành Phố Hồ Chí MinhNasopharyngeal carcinoma (NPC) is a rare type of tumor originating from the region behind the nasal cavity and above the throat. The primary causes of NPC include Epstein-Barr virus infection, genetic mutations, and environmental factors, such as lifestyle. Global and Vietnamese studies have indicated enhanced methylation in the promoter region of CpG islands, contributing to the development of various cancers, including NPC (Chan, Teo, & Johnson, 2002). The p16INK4α gene, also known as cyclin-dependent kinase inhibitor, is a tumor suppressor gene that inhibits Cyclin-Dependent Kinase (CDK), leading to the inhibition of CDK interaction with cyclin, thereby inhibiting the phosphorylation process on pRb, halting the cell cycle. The p16INK4α gene is located on the short arm of chromosome 9 at position 9p21.3, consisting of three exons encoding 156 amino acids (Coppé et al., 2011). According to a meta-analysis of 11 papers on methylation processes on the p16 INK4α gene conducted by Xiao et al. in 2016, significantly higher levels of methylation were found in NPC tissue samples compared to non-tumor samples (Xiao et al., 2016). Additionally, methylation on the p16INK4α gene in blood samples from NPC patients was also significantly higher compared to normal blood samples. Therefore, this article aims to provide further scientific evidence on the enhanced methylation status in the promoter region of CpG islands of NPC patients based on the analysis of the p16INK4α gene using statistical synthesis methods. The research results demonstrated an uneven expression frequency of the p16INK4α gene between the two patient groups and the control group. In the patient group, we observed a Q value of 104.5223 with degrees of freedom (DF) = 13 and a p-value < 0.0001, along with an I2 index of 87.56%, with a 95% confidence interval for I2 from 80.84 to 91.9. Meanwhile, in the control group, the Q value was 56.1888 (p < 0.001), and the I2 index reached 76.86%, with a 95% confidence interval for I2 from 61.38 to 86.14. The weighted frequency of the patient and control groups was 63.478% and 7.966%, respectively, indicating a significant difference between the patient and control groups, with a high Q value and a very low p-value. Other factors that may influence the methylation process, such as ethnicity and sample source, were also included in the analysis. The results showed a significant correlation between the methylation process in these two groups and the development of NPC. Specifically, the Odds Ratios (OR) for China and Africa were 7.002 and 6.47, respectively. We also observed that the OR for samples from the mucosa and body fluids were 6.802 and 5.516, respectively. The analysis confirmed that the expression of the p16INK4α gene is strongly related to the formation of throat cancer. Therefore, this data provides a foundation for experimental studies on NPC samples collected from the Vietnamese population.https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/3040phân tích tổng hợpp16ink4α (cyclin dependent kinase inhibitor 2a)ung thư vòm họngviệt nam
spellingShingle	Lao Đức Thuận Nguyễn Thị Ngọc Thảo Thiều Hồng Huệ Nguyễn Trung Hiếu Phạm Thị Thúy Ngọc Trần Bích Thư Lê Huyền Ái Thúy Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ phân tích tổng hợp p16ink4α (cyclin dependent kinase inhibitor 2a) ung thư vòm họng việt nam
title	Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer
title_full	Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer
title_fullStr	Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer
title_full_unstemmed	Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer
title_short	Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer
title_sort	meta analysis p16ink4α cyclin dependent kinase inhibitor 2a is one of the major causes of nasopharyngeal cancer
topic	phân tích tổng hợp p16ink4α (cyclin dependent kinase inhibitor 2a) ung thư vòm họng việt nam
url	https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/3040
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Meta-analysis: p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) is one of the major causes of nasopharyngeal cancer

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