Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice
Type 2 diabetes mellitus (T2DM) is a metabolic disease caused by a glycolipid metabolism disorder and islet β-cell dysfunction. SCP-80-I is a biologically active water-soluble polysaccharide isolated from sweet corncob, an agricultural byproduct. The hypoglycemic effects of SCP-80-I on T2DM mice and...
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250130 |
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| author | Xin Wang Weiye Xiu Ye Han Zhili Wang Yu Luo Yongqiang Ma |
| author_facet | Xin Wang Weiye Xiu Ye Han Zhili Wang Yu Luo Yongqiang Ma |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Type 2 diabetes mellitus (T2DM) is a metabolic disease caused by a glycolipid metabolism disorder and islet β-cell dysfunction. SCP-80-I is a biologically active water-soluble polysaccharide isolated from sweet corncob, an agricultural byproduct. The hypoglycemic effects of SCP-80-I on T2DM mice and its mechanisms were investigated in this study. SCP-80-I was found to significantly reduce blood glucose and lipid deposition levels in T2DM mice, as well as decrease serum leptin and increase adiponectin secretion. Interestingly, real time-polymerase chain reaction (RT-PCR) and Western blotting results revealed that SCP-80-I could regulate the expression of several glycolipid metabolisms and insulin secretion genes and proteins, including 5’-AMP-activated protein kinase (AMPK), carnitine palmitoyltransferase I (CPT1), and acetyl coenzyme A carboxylase (ACC) in the liver and AMPK, sirtuin 1 (Sirt1), peroxisome proliferator-activated receptorycoactivator-1 (PGC-1α), and uncoupling protein 2 (UCP2) in the pancreas. To have a hypoglycemic effect, SCP-80-I regulated glycolipid metabolism and islet cell function in the liver by regulating the AMPK/ACC/CPT1 signaling pathway and the AMPK/Sirt1/PGC-1α and AMPK/Sirt1/UCP2 signaling pathways. These findings improve our understanding of polysaccharides derived from sweet corncob and the use of SCP-80-I in the production of hypoglycemic foods. |
| format | Article |
| id | doaj-art-479723d5183f416eb8f1b0e87afaaf3e |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-479723d5183f416eb8f1b0e87afaaf3e2025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331543155510.26599/FSHW.2022.9250130Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic miceXin Wang0Weiye Xiu1Ye Han2Zhili Wang3Yu Luo4Yongqiang Ma5Heilongjiang Provincial Key Laboratory of Cereals and Comprehensive Processing of Cereal Resources, College of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaHeilongjiang Provincial Key Laboratory of Cereals and Comprehensive Processing of Cereal Resources, College of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaHeilongjiang Provincial Key Laboratory of Cereals and Comprehensive Processing of Cereal Resources, College of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaHeilongjiang Provincial Key Laboratory of Cereals and Comprehensive Processing of Cereal Resources, College of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaHeilongjiang Provincial Key Laboratory of Cereals and Comprehensive Processing of Cereal Resources, College of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaHeilongjiang Provincial Key Laboratory of Cereals and Comprehensive Processing of Cereal Resources, College of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaType 2 diabetes mellitus (T2DM) is a metabolic disease caused by a glycolipid metabolism disorder and islet β-cell dysfunction. SCP-80-I is a biologically active water-soluble polysaccharide isolated from sweet corncob, an agricultural byproduct. The hypoglycemic effects of SCP-80-I on T2DM mice and its mechanisms were investigated in this study. SCP-80-I was found to significantly reduce blood glucose and lipid deposition levels in T2DM mice, as well as decrease serum leptin and increase adiponectin secretion. Interestingly, real time-polymerase chain reaction (RT-PCR) and Western blotting results revealed that SCP-80-I could regulate the expression of several glycolipid metabolisms and insulin secretion genes and proteins, including 5’-AMP-activated protein kinase (AMPK), carnitine palmitoyltransferase I (CPT1), and acetyl coenzyme A carboxylase (ACC) in the liver and AMPK, sirtuin 1 (Sirt1), peroxisome proliferator-activated receptorycoactivator-1 (PGC-1α), and uncoupling protein 2 (UCP2) in the pancreas. To have a hypoglycemic effect, SCP-80-I regulated glycolipid metabolism and islet cell function in the liver by regulating the AMPK/ACC/CPT1 signaling pathway and the AMPK/Sirt1/PGC-1α and AMPK/Sirt1/UCP2 signaling pathways. These findings improve our understanding of polysaccharides derived from sweet corncob and the use of SCP-80-I in the production of hypoglycemic foods.https://www.sciopen.com/article/10.26599/FSHW.2022.9250130sweet corn cob polysaccharidetype 2 diabetessignal pathway5’-amp-activated protein kinase (ampk) |
| spellingShingle | Xin Wang Weiye Xiu Ye Han Zhili Wang Yu Luo Yongqiang Ma Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice Food Science and Human Wellness sweet corn cob polysaccharide type 2 diabetes signal pathway 5’-amp-activated protein kinase (ampk) |
| title | Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice |
| title_full | Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice |
| title_fullStr | Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice |
| title_full_unstemmed | Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice |
| title_short | Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice |
| title_sort | hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high fat diet and streptozotocin induced diabetic mice |
| topic | sweet corn cob polysaccharide type 2 diabetes signal pathway 5’-amp-activated protein kinase (ampk) |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250130 |
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