Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysis
Background Diabetic cataract (DC) is a major cause of blindness, with its pathogenesis involving oxidative stress and ferroptosis, according to recent studies.Methods We performed a Mendelian Randomization (MR) study using GWAS data to select SNPs and assess the causal link between diabetes and cata...
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Taylor & Francis Group
2024-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2024.2420563 |
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| author | Wenlan Liu Yiming Pan |
| author_facet | Wenlan Liu Yiming Pan |
| author_sort | Wenlan Liu |
| collection | DOAJ |
| description | Background Diabetic cataract (DC) is a major cause of blindness, with its pathogenesis involving oxidative stress and ferroptosis, according to recent studies.Methods We performed a Mendelian Randomization (MR) study using GWAS data to select SNPs and assess the causal link between diabetes and cataracts. DC datasets were analyzed for differential gene expression, WGCNA, and protein-protein interactions to identify key oxidative stress and ferroptosis genes. An SVM-RFE algorithm developed a diagnostic model, and ImmuCellAI analyzed immune infiltration patterns.Results MR analysis confirmed diabetes as a cataract risk factor and identified core genes related to oxidative stress and ferroptosis in DC. Four key genes (Hspa5/Nfe2l2/Atf3/Stat3) linked to both processes were discovered. Immune infiltration analysis revealed an imbalance associated with these genes.Conclusions A functional interaction between oxidative stress and ferroptosis genes in DC is suggested, with a 4-gene model, indicating their potential as a ‘bridge' in DC pathogenesis. |
| format | Article |
| id | doaj-art-46f40c655eec4b64978dcbcf290e069a |
| institution | Kabale University |
| issn | 1351-0002 1743-2928 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj-art-46f40c655eec4b64978dcbcf290e069a2024-12-07T06:20:30ZengTaylor & Francis GroupRedox Report1351-00021743-29282024-12-0129110.1080/13510002.2024.2420563Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysisWenlan Liu0Yiming Pan1College of Medical Technology, Xi'an Medical University, Xi'an, People’s Republic of ChinaCollege of Medical Technology, Xi'an Medical University, Xi'an, People’s Republic of ChinaBackground Diabetic cataract (DC) is a major cause of blindness, with its pathogenesis involving oxidative stress and ferroptosis, according to recent studies.Methods We performed a Mendelian Randomization (MR) study using GWAS data to select SNPs and assess the causal link between diabetes and cataracts. DC datasets were analyzed for differential gene expression, WGCNA, and protein-protein interactions to identify key oxidative stress and ferroptosis genes. An SVM-RFE algorithm developed a diagnostic model, and ImmuCellAI analyzed immune infiltration patterns.Results MR analysis confirmed diabetes as a cataract risk factor and identified core genes related to oxidative stress and ferroptosis in DC. Four key genes (Hspa5/Nfe2l2/Atf3/Stat3) linked to both processes were discovered. Immune infiltration analysis revealed an imbalance associated with these genes.Conclusions A functional interaction between oxidative stress and ferroptosis genes in DC is suggested, with a 4-gene model, indicating their potential as a ‘bridge' in DC pathogenesis.https://www.tandfonline.com/doi/10.1080/13510002.2024.2420563Diabetic cataractoxidative stressferroptosisimmune infiltrationMendelian randomizationHspa5 |
| spellingShingle | Wenlan Liu Yiming Pan Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysis Redox Report Diabetic cataract oxidative stress ferroptosis immune infiltration Mendelian randomization Hspa5 |
| title | Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysis |
| title_full | Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysis |
| title_fullStr | Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysis |
| title_full_unstemmed | Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysis |
| title_short | Unraveling the mechanisms underlying diabetic cataracts: insights from Mendelian randomization analysis |
| title_sort | unraveling the mechanisms underlying diabetic cataracts insights from mendelian randomization analysis |
| topic | Diabetic cataract oxidative stress ferroptosis immune infiltration Mendelian randomization Hspa5 |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2024.2420563 |
| work_keys_str_mv | AT wenlanliu unravelingthemechanismsunderlyingdiabeticcataractsinsightsfrommendelianrandomizationanalysis AT yimingpan unravelingthemechanismsunderlyingdiabeticcataractsinsightsfrommendelianrandomizationanalysis |