A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging
Background: Preclinical cell tracking is enhanced with a multimodal imaging approach. Bioluminescence imaging (BLI) is a highly sensitive optical modality that relies on engineering cells to constitutively express a luciferase gene. Magnetic particle imaging (MPI) is a newer imaging modality that di...
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MDPI AG
2024-11-01
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| Series: | Tomography |
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| Online Access: | https://www.mdpi.com/2379-139X/10/11/135 |
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| author | Sophia Trozzo Bijita Neupane Paula J. Foster |
| author_facet | Sophia Trozzo Bijita Neupane Paula J. Foster |
| author_sort | Sophia Trozzo |
| collection | DOAJ |
| description | Background: Preclinical cell tracking is enhanced with a multimodal imaging approach. Bioluminescence imaging (BLI) is a highly sensitive optical modality that relies on engineering cells to constitutively express a luciferase gene. Magnetic particle imaging (MPI) is a newer imaging modality that directly detects superparamagnetic iron oxide (SPIO) particles used to label cells. Here, we compare BLI and MPI for imaging cells in vitro and in vivo. Methods: Mouse 4T1 breast carcinoma cells were transduced to express firefly luciferase, labeled with SPIO (ProMag), and imaged as cell samples after subcutaneous injection into mice. Results: For cell samples, the BLI and MPI signals were strongly correlated with cell number. Both modalities presented limitations for imaging cells in vivo. For BLI, weak signal penetration, signal attenuation, and scattering prevented the detection of cells for mice with hair and for cells far from the tissue surface. For MPI, background signals obscured the detection of low cell numbers due to the limited dynamic range, and cell numbers could not be accurately quantified from in vivo images. Conclusions: It is important to understand the shortcomings of these imaging modalities to develop strategies to improve cellular detection sensitivity. |
| format | Article |
| id | doaj-art-46d6bbcbde3f458c8b1de7db5b2bc6be |
| institution | Kabale University |
| issn | 2379-1381 2379-139X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Tomography |
| spelling | doaj-art-46d6bbcbde3f458c8b1de7db5b2bc6be2024-11-26T18:23:39ZengMDPI AGTomography2379-13812379-139X2024-11-0110111846186610.3390/tomography10110135A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence ImagingSophia Trozzo0Bijita Neupane1Paula J. Foster2Department of Medical Biophysics, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 3K7, CanadaDepartment of Medical Biophysics, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 3K7, CanadaDepartment of Medical Biophysics, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 3K7, CanadaBackground: Preclinical cell tracking is enhanced with a multimodal imaging approach. Bioluminescence imaging (BLI) is a highly sensitive optical modality that relies on engineering cells to constitutively express a luciferase gene. Magnetic particle imaging (MPI) is a newer imaging modality that directly detects superparamagnetic iron oxide (SPIO) particles used to label cells. Here, we compare BLI and MPI for imaging cells in vitro and in vivo. Methods: Mouse 4T1 breast carcinoma cells were transduced to express firefly luciferase, labeled with SPIO (ProMag), and imaged as cell samples after subcutaneous injection into mice. Results: For cell samples, the BLI and MPI signals were strongly correlated with cell number. Both modalities presented limitations for imaging cells in vivo. For BLI, weak signal penetration, signal attenuation, and scattering prevented the detection of cells for mice with hair and for cells far from the tissue surface. For MPI, background signals obscured the detection of low cell numbers due to the limited dynamic range, and cell numbers could not be accurately quantified from in vivo images. Conclusions: It is important to understand the shortcomings of these imaging modalities to develop strategies to improve cellular detection sensitivity.https://www.mdpi.com/2379-139X/10/11/135cell trackingmagnetic particle imagingbioluminescence imagingmultimodal imagingsensitivitycell detection |
| spellingShingle | Sophia Trozzo Bijita Neupane Paula J. Foster A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging Tomography cell tracking magnetic particle imaging bioluminescence imaging multimodal imaging sensitivity cell detection |
| title | A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging |
| title_full | A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging |
| title_fullStr | A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging |
| title_full_unstemmed | A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging |
| title_short | A Comparison of the Sensitivity and Cellular Detection Capabilities of Magnetic Particle Imaging and Bioluminescence Imaging |
| title_sort | comparison of the sensitivity and cellular detection capabilities of magnetic particle imaging and bioluminescence imaging |
| topic | cell tracking magnetic particle imaging bioluminescence imaging multimodal imaging sensitivity cell detection |
| url | https://www.mdpi.com/2379-139X/10/11/135 |
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