Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollen
Ultrasound is an efficient and eco-friendly friendly non-thermal technology for enhancing the extraction of bioactive ingredients from food. This study explored the impact of ultrasound on the microstructure and antioxidant properties of camellia bee pollen. Additionally, the impact of key contribut...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725001385 |
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| author | Yanxiang Bi Shiye Luo Jiabao Ni Song Miao Zhen Ning Zhihao Zhang Sijia Xu Wenli Tian Wenjun Peng Xiaoming Fang |
| author_facet | Yanxiang Bi Shiye Luo Jiabao Ni Song Miao Zhen Ning Zhihao Zhang Sijia Xu Wenli Tian Wenjun Peng Xiaoming Fang |
| author_sort | Yanxiang Bi |
| collection | DOAJ |
| description | Ultrasound is an efficient and eco-friendly friendly non-thermal technology for enhancing the extraction of bioactive ingredients from food. This study explored the impact of ultrasound on the microstructure and antioxidant properties of camellia bee pollen. Additionally, the impact of key contributors to antioxidant activity was examined through non-targeted metabolomics analysis. The results showed that ultrasonic exposure progressively degraded the cell walls of bee pollen, resulting in severe collapse of the intine. Notably, this degradation concurrently facilitated the release of polyphenols and flavonoids. The DPPH and ABTS radical scavenging capacity reached the highest after 40 and 60 min of ultrasonic treatment. After 40 min of ultrasonic treatment, the MDA content in camellia bee pollen exhibited a significant rise of 33.47 % compared to the control group, while it further escalated by 57.07 % after 60 min of ultrasonic treatment. Non-targeted metabolomics analysis identified a total of 7 differential metabolites that serve as potential biomarkers for ultrasonic-treated camellia bee pollen. Further analysis of the purine and nucleotide metabolism pathway indicated that the antioxidant defense systems within camellia bee pollen were activated by ultrasonic treatment, leading to a significant enhancement in its antioxidant capacity. These findings establish a solid foundation for the advancement of ultrasound treatment as a novel and green technology to improve the biological activities and qualities of bee pollen. |
| format | Article |
| id | doaj-art-45fd4bf10f954e16a3f260e02eb76ee7 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-45fd4bf10f954e16a3f260e02eb76ee72025-08-20T03:47:25ZengElsevierUltrasonics Sonochemistry1350-41772025-07-0111810735910.1016/j.ultsonch.2025.107359Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollenYanxiang Bi0Shiye Luo1Jiabao Ni2Song Miao3Zhen Ning4Zhihao Zhang5Sijia Xu6Wenli Tian7Wenjun Peng8Xiaoming Fang9State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; College of Engineering, China Agricultural University, P.O. Box 194, 17 Qinghua Donglu, Beijing 100083, ChinaTeagasc Food Research Center Moorepark, Fermoy, Co.Cork P61C996, IrelandCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fujian 350002, ChinaMudanjiang Branch of Heilongjiang Academy of Agricltural Sciences, Heilongjiang 157020, ChinaAgricultural and Rural Bureau of Linping District, Hangzhou City, Zhejiang 311103, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Corresponding authors.State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Corresponding authors.Ultrasound is an efficient and eco-friendly friendly non-thermal technology for enhancing the extraction of bioactive ingredients from food. This study explored the impact of ultrasound on the microstructure and antioxidant properties of camellia bee pollen. Additionally, the impact of key contributors to antioxidant activity was examined through non-targeted metabolomics analysis. The results showed that ultrasonic exposure progressively degraded the cell walls of bee pollen, resulting in severe collapse of the intine. Notably, this degradation concurrently facilitated the release of polyphenols and flavonoids. The DPPH and ABTS radical scavenging capacity reached the highest after 40 and 60 min of ultrasonic treatment. After 40 min of ultrasonic treatment, the MDA content in camellia bee pollen exhibited a significant rise of 33.47 % compared to the control group, while it further escalated by 57.07 % after 60 min of ultrasonic treatment. Non-targeted metabolomics analysis identified a total of 7 differential metabolites that serve as potential biomarkers for ultrasonic-treated camellia bee pollen. Further analysis of the purine and nucleotide metabolism pathway indicated that the antioxidant defense systems within camellia bee pollen were activated by ultrasonic treatment, leading to a significant enhancement in its antioxidant capacity. These findings establish a solid foundation for the advancement of ultrasound treatment as a novel and green technology to improve the biological activities and qualities of bee pollen.http://www.sciencedirect.com/science/article/pii/S1350417725001385Camellia bee pollenUltrasound treatmentMicrostructureBioactive componentsAntioxidant activitiesMetabolomics analysis |
| spellingShingle | Yanxiang Bi Shiye Luo Jiabao Ni Song Miao Zhen Ning Zhihao Zhang Sijia Xu Wenli Tian Wenjun Peng Xiaoming Fang Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollen Ultrasonics Sonochemistry Camellia bee pollen Ultrasound treatment Microstructure Bioactive components Antioxidant activities Metabolomics analysis |
| title | Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollen |
| title_full | Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollen |
| title_fullStr | Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollen |
| title_full_unstemmed | Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollen |
| title_short | Effect of ultrasonic treatment on the microstructure, antioxidant activities and metabolites of camellia bee pollen |
| title_sort | effect of ultrasonic treatment on the microstructure antioxidant activities and metabolites of camellia bee pollen |
| topic | Camellia bee pollen Ultrasound treatment Microstructure Bioactive components Antioxidant activities Metabolomics analysis |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725001385 |
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