Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and Acetochlor

Prometryn and acetochlor are herbicides used to control weeds in farmlands and other areas. They enter the soil through direct application, residual accumulation in crops, and atmospheric deposition. The pollution of their residues in the environment has attracted people’s attention. Bioremediation...

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Main Authors: Yue Cheng, Qian Fu, Junjia Xu, Xinhua Niu, Lin Liu, Jiaqi Wang, Jingwen Quan, Qingyue Yu, Baoyan Chi, Haitao Li, Rongmei Liu
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/7/1698
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author Yue Cheng
Qian Fu
Junjia Xu
Xinhua Niu
Lin Liu
Jiaqi Wang
Jingwen Quan
Qingyue Yu
Baoyan Chi
Haitao Li
Rongmei Liu
author_facet Yue Cheng
Qian Fu
Junjia Xu
Xinhua Niu
Lin Liu
Jiaqi Wang
Jingwen Quan
Qingyue Yu
Baoyan Chi
Haitao Li
Rongmei Liu
author_sort Yue Cheng
collection DOAJ
description Prometryn and acetochlor are herbicides used to control weeds in farmlands and other areas. They enter the soil through direct application, residual accumulation in crops, and atmospheric deposition. The pollution of their residues in the environment has attracted people’s attention. Bioremediation is one of the main methods to solve such problems. In this study, the effects of prometryn and acetochlor-degrading strain NM-1 on soil enzymes, soil microbial communities, and physiological indexes of soybean seedlings during soil remediation were studied, and the relationship between them was discussed. The results showed that 81.54% of prometryn (50 mg·L<sup>−1</sup>) and 89.47% of acetochlor (50 mg·L<sup>−1</sup>) were degraded within 15 days after NM-1 inoculation in soil. NM-1 positively affected soil enzyme activities and soil microbial communities, and the abundance of beneficial bacteria in soil increased. More importantly, the inoculation of strain NM-1 under prometryn and acetochlor stress significantly increased plant height, root length, root volume, water content, chlorophyll concentration, and root activity of soybean. The results of these studies showed that the NM-1 strain showed significant potential in bioremediation in order to provide technical support for solving the problem of prometryn and acetochlor pollution.
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institution Kabale University
issn 2076-2607
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spelling doaj-art-45cb1e2251fc4897a10b0f08733ccf582025-08-20T03:56:46ZengMDPI AGMicroorganisms2076-26072025-07-01137169810.3390/microorganisms13071698Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and AcetochlorYue Cheng0Qian Fu1Junjia Xu2Xinhua Niu3Lin Liu4Jiaqi Wang5Jingwen Quan6Qingyue Yu7Baoyan Chi8Haitao Li9Rongmei Liu10Heilongjiang Green Food Science Research Institute, Harbin 150023, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Green Food Science Research Institute, Harbin 150023, ChinaPrometryn and acetochlor are herbicides used to control weeds in farmlands and other areas. They enter the soil through direct application, residual accumulation in crops, and atmospheric deposition. The pollution of their residues in the environment has attracted people’s attention. Bioremediation is one of the main methods to solve such problems. In this study, the effects of prometryn and acetochlor-degrading strain NM-1 on soil enzymes, soil microbial communities, and physiological indexes of soybean seedlings during soil remediation were studied, and the relationship between them was discussed. The results showed that 81.54% of prometryn (50 mg·L<sup>−1</sup>) and 89.47% of acetochlor (50 mg·L<sup>−1</sup>) were degraded within 15 days after NM-1 inoculation in soil. NM-1 positively affected soil enzyme activities and soil microbial communities, and the abundance of beneficial bacteria in soil increased. More importantly, the inoculation of strain NM-1 under prometryn and acetochlor stress significantly increased plant height, root length, root volume, water content, chlorophyll concentration, and root activity of soybean. The results of these studies showed that the NM-1 strain showed significant potential in bioremediation in order to provide technical support for solving the problem of prometryn and acetochlor pollution.https://www.mdpi.com/2076-2607/13/7/1698prometrynacetochlormicrobial bioremediationsoil enzyme activitysoil microbial communitysoybean seedlings
spellingShingle Yue Cheng
Qian Fu
Junjia Xu
Xinhua Niu
Lin Liu
Jiaqi Wang
Jingwen Quan
Qingyue Yu
Baoyan Chi
Haitao Li
Rongmei Liu
Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and Acetochlor
Microorganisms
prometryn
acetochlor
microbial bioremediation
soil enzyme activity
soil microbial community
soybean seedlings
title Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and Acetochlor
title_full Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and Acetochlor
title_fullStr Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and Acetochlor
title_full_unstemmed Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and Acetochlor
title_short Possible Use in Soil Bioremediation of the Bacterial Strain Bacillus Sphaericus NM-1 Capable of Simultaneously Degrading Promethrin and Acetochlor
title_sort possible use in soil bioremediation of the bacterial strain bacillus sphaericus nm 1 capable of simultaneously degrading promethrin and acetochlor
topic prometryn
acetochlor
microbial bioremediation
soil enzyme activity
soil microbial community
soybean seedlings
url https://www.mdpi.com/2076-2607/13/7/1698
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