The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol
The National Cancer Institute (NCI) recognizes the potential of technologies based on the use of nanoparticles (NPs) in revolutionizing clinical approaches to the diagnosis and prognosis of cancer. Recent research suggests that once NPs come into contact with the biological fluid of cancer patients,...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/13/12/1024 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846105697104691200 |
|---|---|
| author | Giulio Bevilacqua Roberta Corvino Anna Laura Capriotti Carmela Maria Montone Martina Moriconi Stefano Salciccia Valentina Brunelli Valerio Santarelli Beatrice Sciarra Aldo Laganà Daniele Santini Alessandro Sciarra Alessandro Gentilucci |
| author_facet | Giulio Bevilacqua Roberta Corvino Anna Laura Capriotti Carmela Maria Montone Martina Moriconi Stefano Salciccia Valentina Brunelli Valerio Santarelli Beatrice Sciarra Aldo Laganà Daniele Santini Alessandro Sciarra Alessandro Gentilucci |
| author_sort | Giulio Bevilacqua |
| collection | DOAJ |
| description | The National Cancer Institute (NCI) recognizes the potential of technologies based on the use of nanoparticles (NPs) in revolutionizing clinical approaches to the diagnosis and prognosis of cancer. Recent research suggests that once NPs come into contact with the biological fluid of cancer patients, they are covered by proteins, forming a “protein corona” composed of hundreds of plasma proteins. The concept of a personalized, disease-specific protein corona, demonstrating substantial differences in NP corona profiles between patients with and without cancer, has been introduced. We developed the design of an experimental prospective single-center study with patients allocated in a 1:1:1 ratio of one of three arms: untreated patients with benign prostatic hyperplasia (BPH), untreated patients with non-metastatic prostate cancer (PCa), and metastatic prostate cancer patients starting systemic therapies with new androgen-targeted agents or taxanes. The protocol aims to develop and implement sensitive nanotools with two distinct objectives: First, to design NPs capable of selectively binding and detecting biomarkers in order to build a predictive diagnostic model to effectively discriminate between patient sera affected by BPH and PCa. Secondly, within the population with PCa, in the case of initial advanced metastatic diagnosis, the objective is to find biomarkers capable of predicting the response to systemic treatments to improve the precision and efficiency of monitoring treatment outcomes. For protein and metabolite corona experiments, we developed a cross-reactive sensor array platform with cancer detection capacity made of three liposomal formulations with different surface charges. For proteomic-NP studies, proteins were identified and quantified using nano-high-performance LC (nanoHPLC) coupled with MS/MS (nanoHPLC−MS/MS). Metabolites were instead analyzed using an untargeted metabolomic approach. Compared with previous review articles, the novelty of this review is represented by the analysis of the possible clinical applications of protein corona NPs focused on PCa and the presentation of a new clinical protocol in the metastatic phase of PCa. |
| format | Article |
| id | doaj-art-45befa2cee734536bed6a0947e9d946f |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-45befa2cee734536bed6a0947e9d946f2024-12-27T14:12:03ZengMDPI AGBiology2079-77372024-12-011312102410.3390/biology13121024The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial ProtocolGiulio Bevilacqua0Roberta Corvino1Anna Laura Capriotti2Carmela Maria Montone3Martina Moriconi4Stefano Salciccia5Valentina Brunelli6Valerio Santarelli7Beatrice Sciarra8Aldo Laganà9Daniele Santini10Alessandro Sciarra11Alessandro Gentilucci12Department “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyDepartment “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyDepartment of Chemistry, University Sapienza, 00161 Rome, ItalyDepartment of Chemistry, University Sapienza, 00161 Rome, ItalyDepartment “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyDepartment “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyDepartment “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyDepartment “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyDepartment of Pharmaceutic Chemistry, University Sapienza, 00161 Rome, ItalyDepartment of Chemistry, University Sapienza, 00161 Rome, ItalyDepartment of Oncology, University Sapienza, 00161 Rome, ItalyDepartment “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyDepartment “Materno Infantile e Scienze Urologiche”, University Sapienza, 00161 Rome, ItalyThe National Cancer Institute (NCI) recognizes the potential of technologies based on the use of nanoparticles (NPs) in revolutionizing clinical approaches to the diagnosis and prognosis of cancer. Recent research suggests that once NPs come into contact with the biological fluid of cancer patients, they are covered by proteins, forming a “protein corona” composed of hundreds of plasma proteins. The concept of a personalized, disease-specific protein corona, demonstrating substantial differences in NP corona profiles between patients with and without cancer, has been introduced. We developed the design of an experimental prospective single-center study with patients allocated in a 1:1:1 ratio of one of three arms: untreated patients with benign prostatic hyperplasia (BPH), untreated patients with non-metastatic prostate cancer (PCa), and metastatic prostate cancer patients starting systemic therapies with new androgen-targeted agents or taxanes. The protocol aims to develop and implement sensitive nanotools with two distinct objectives: First, to design NPs capable of selectively binding and detecting biomarkers in order to build a predictive diagnostic model to effectively discriminate between patient sera affected by BPH and PCa. Secondly, within the population with PCa, in the case of initial advanced metastatic diagnosis, the objective is to find biomarkers capable of predicting the response to systemic treatments to improve the precision and efficiency of monitoring treatment outcomes. For protein and metabolite corona experiments, we developed a cross-reactive sensor array platform with cancer detection capacity made of three liposomal formulations with different surface charges. For proteomic-NP studies, proteins were identified and quantified using nano-high-performance LC (nanoHPLC) coupled with MS/MS (nanoHPLC−MS/MS). Metabolites were instead analyzed using an untargeted metabolomic approach. Compared with previous review articles, the novelty of this review is represented by the analysis of the possible clinical applications of protein corona NPs focused on PCa and the presentation of a new clinical protocol in the metastatic phase of PCa.https://www.mdpi.com/2079-7737/13/12/1024protein coronananoparticlesprostate neoplasm |
| spellingShingle | Giulio Bevilacqua Roberta Corvino Anna Laura Capriotti Carmela Maria Montone Martina Moriconi Stefano Salciccia Valentina Brunelli Valerio Santarelli Beatrice Sciarra Aldo Laganà Daniele Santini Alessandro Sciarra Alessandro Gentilucci The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol Biology protein corona nanoparticles prostate neoplasm |
| title | The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol |
| title_full | The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol |
| title_fullStr | The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol |
| title_full_unstemmed | The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol |
| title_short | The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol |
| title_sort | protein corona on nanoparticles for tumor targeting in prostate cancer a review of the literature and experimental trial protocol |
| topic | protein corona nanoparticles prostate neoplasm |
| url | https://www.mdpi.com/2079-7737/13/12/1024 |
| work_keys_str_mv | AT giuliobevilacqua theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT robertacorvino theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT annalauracapriotti theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT carmelamariamontone theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT martinamoriconi theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT stefanosalciccia theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT valentinabrunelli theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT valeriosantarelli theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT beatricesciarra theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT aldolagana theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT danielesantini theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT alessandrosciarra theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT alessandrogentilucci theproteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT giuliobevilacqua proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT robertacorvino proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT annalauracapriotti proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT carmelamariamontone proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT martinamoriconi proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT stefanosalciccia proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT valentinabrunelli proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT valeriosantarelli proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT beatricesciarra proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT aldolagana proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT danielesantini proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT alessandrosciarra proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol AT alessandrogentilucci proteincoronaonnanoparticlesfortumortargetinginprostatecancerareviewoftheliteratureandexperimentaltrialprotocol |