Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
We aimed to investigate galectin-1 overexpression induces normal fibroblasts (NFs) translates into cancer-associated fibroblasts (CAFs). Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell. The motilities of H1299 and A549 cells were measured. Human umbilical vein...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Cell Adhesion & Migration |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19336918.2024.2335881 |
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| author | Lei Zhang Wenbang Chen Xiaojun Li Gengming Wang Fubao Xing Xiao Zhu |
| author_facet | Lei Zhang Wenbang Chen Xiaojun Li Gengming Wang Fubao Xing Xiao Zhu |
| author_sort | Lei Zhang |
| collection | DOAJ |
| description | We aimed to investigate galectin-1 overexpression induces normal fibroblasts (NFs) translates into cancer-associated fibroblasts (CAFs). Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell. The motilities of H1299 and A549 cells were measured. Human umbilical vein endothelial cell (HUVEC) proliferation and tube formation ability were assessed. Tumor volume and tumor weight was recorded. Cells motilities were increased, while apoptosis rates were decreased after CMs co-cultured. B-cell lymphoma-2 (Bcl-2) expression level was increased, while Bcl2-associatedX (Bax) and cleaved-caspase3 decreased. CMs treatment enhanced HUVEC proliferation and tube formation. Tumor volume and weight in CMs treated mice were increased, and the sensitivity of anlotinib in co-cultured cells was decreased. Our results revealed that galectin-1 overexpression induced NFs translated into CAFs. |
| format | Article |
| id | doaj-art-45bd411b7a7e47d5995a37643de20f3f |
| institution | Kabale University |
| issn | 1933-6918 1933-6926 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj-art-45bd411b7a7e47d5995a37643de20f3f2024-11-25T08:41:13ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262024-12-0118111110.1080/19336918.2024.2335881Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancerLei Zhang0Wenbang Chen1Xiaojun Li2Gengming Wang3Fubao Xing4Xiao Zhu50Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China0Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China0Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China0Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China0Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, ChinaWe aimed to investigate galectin-1 overexpression induces normal fibroblasts (NFs) translates into cancer-associated fibroblasts (CAFs). Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell. The motilities of H1299 and A549 cells were measured. Human umbilical vein endothelial cell (HUVEC) proliferation and tube formation ability were assessed. Tumor volume and tumor weight was recorded. Cells motilities were increased, while apoptosis rates were decreased after CMs co-cultured. B-cell lymphoma-2 (Bcl-2) expression level was increased, while Bcl2-associatedX (Bax) and cleaved-caspase3 decreased. CMs treatment enhanced HUVEC proliferation and tube formation. Tumor volume and weight in CMs treated mice were increased, and the sensitivity of anlotinib in co-cultured cells was decreased. Our results revealed that galectin-1 overexpression induced NFs translated into CAFs.https://www.tandfonline.com/doi/10.1080/19336918.2024.2335881Condition mediumsHUVECTGF-β1tube formationα-SMA |
| spellingShingle | Lei Zhang Wenbang Chen Xiaojun Li Gengming Wang Fubao Xing Xiao Zhu Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer Cell Adhesion & Migration Condition mediums HUVEC TGF-β1 tube formation α-SMA |
| title | Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer |
| title_full | Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer |
| title_fullStr | Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer |
| title_full_unstemmed | Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer |
| title_short | Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer |
| title_sort | galectin 1 overexpression induces normal fibroblasts translate into cancer associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer |
| topic | Condition mediums HUVEC TGF-β1 tube formation α-SMA |
| url | https://www.tandfonline.com/doi/10.1080/19336918.2024.2335881 |
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