Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice
BACKGROUND: Obstructive jaundice, often due to choledocholithiasis or malignancies, leads to immune suppression, intestinal damage, and bacterial translocation, worsening outcomes. Some inflammatory mediators like nuclear factor kappa B (NF-κB), alpha-smooth muscle actin (α-SMA), and interleukin-6 (...
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Secretariat of The Indonesian Biomedical Journal
2024-12-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/3384 |
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| author | Avit Suchitra Alvarino Alvarino Eryati Darwin Harnavi Harun Muhammad Iqbal Rivai Ade Sukma |
| author_facet | Avit Suchitra Alvarino Alvarino Eryati Darwin Harnavi Harun Muhammad Iqbal Rivai Ade Sukma |
| author_sort | Avit Suchitra |
| collection | DOAJ |
| description | BACKGROUND: Obstructive jaundice, often due to choledocholithiasis or malignancies, leads to immune suppression, intestinal damage, and bacterial translocation, worsening outcomes. Some inflammatory mediators like nuclear factor kappa B (NF-κB), alpha-smooth muscle actin (α-SMA), and interleukin-6 (IL-6) are important in this process. Current treatments remain inadequate, highlighting the need for novel approaches. Probiotics, such as Lactococcus lactis D4 (LLD4), may help reduce inflammation and bacterial translocation, thus offering a potential therapeutic option. This study was conducted to evaluate the effects of LLD4 on NF-κB, α-SMA, and IL-6 in obstructive jaundice rat models.
METHODS: This post-test randomized controlled study involved 15 male Wistar rats divided into three groups: sham, bile duct ligation (BDL), and BDL+LLD4 groups. The rats were maintained for 7–10 days, with the rats in BDL+LLD4 group received fermented milk containing LLD4 via gavage at a dose of 112 mg/20 gBW per day for 7 days. The expression levels of NF-κB, α-SMA, and IL-6 were analyzed using immunohistochemistry.
RESULTS: Administration of LLD4 were able to significantly reduced NF-κB expression compared to the BDL group (40.20±21.276 vs. 53.60 ± 20.403) in obstructive jaundice rat models. Though not significant, BDL+LLD4 group showed lower α-SMA expression compared to BDL group (58.40±14.271 vs. 63.20±9.16). However, administration of LLD4 did not give any significant effect on IL-6 expression.
CONCLUSION: LLD4 reduces inflammation in models of obstructive jaundice by lowering the NF-κB and α-SMA expression. This indicates that LLD4 might be potential as an adjunct therapy for reducing morbidity in obstructive jaundice cases.
KEYWORDS: obstructive jaundice, bile duct ligation, Lactococcus lactis D4, NF-κB, α-SMA, IL-6 |
| format | Article |
| id | doaj-art-45978bf27702423fb3c5ceebf55afce4 |
| institution | Kabale University |
| issn | 2085-3297 2355-9179 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj-art-45978bf27702423fb3c5ceebf55afce42025-01-06T02:04:18ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792024-12-01166540510.18585/inabj.v16i6.3384608Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive JaundiceAvit Suchitra0Alvarino Alvarino1Eryati Darwin2Harnavi Harun3Muhammad Iqbal Rivai4Ade Sukma5Division of Digestive, Department of Surgery, Faculty of Medicine, Universitas Andalas, Jl. Perintis Kemerdekaan No. 94, Padang 25163Division of Urology, Department of Surgery, Faculty of Medicine, Universitas Andalas, Jl. Perintis Kemerdekaan No. 94, Padang 25163Histology Department, Faculty of Medicine, Universitas Andalas, Jl. Perintis Kemerdekaan No. 94, Padang 25163Internal Medicine Department, Faculty of Medicine, Universitas Andalas, Jl. Perintis Kemerdekaan No. 94, Padang 25163Division of Digestive, Department of Surgery, Faculty of Medicine, Universitas Andalas, Jl. Perintis Kemerdekaan No. 94, Padang 25163Faculty of Animal Science, Universitas Andalas, Jl. Prof. Dr. Hamka Kampus Unand Limau Manis, Padang 25163BACKGROUND: Obstructive jaundice, often due to choledocholithiasis or malignancies, leads to immune suppression, intestinal damage, and bacterial translocation, worsening outcomes. Some inflammatory mediators like nuclear factor kappa B (NF-κB), alpha-smooth muscle actin (α-SMA), and interleukin-6 (IL-6) are important in this process. Current treatments remain inadequate, highlighting the need for novel approaches. Probiotics, such as Lactococcus lactis D4 (LLD4), may help reduce inflammation and bacterial translocation, thus offering a potential therapeutic option. This study was conducted to evaluate the effects of LLD4 on NF-κB, α-SMA, and IL-6 in obstructive jaundice rat models. METHODS: This post-test randomized controlled study involved 15 male Wistar rats divided into three groups: sham, bile duct ligation (BDL), and BDL+LLD4 groups. The rats were maintained for 7–10 days, with the rats in BDL+LLD4 group received fermented milk containing LLD4 via gavage at a dose of 112 mg/20 gBW per day for 7 days. The expression levels of NF-κB, α-SMA, and IL-6 were analyzed using immunohistochemistry. RESULTS: Administration of LLD4 were able to significantly reduced NF-κB expression compared to the BDL group (40.20±21.276 vs. 53.60 ± 20.403) in obstructive jaundice rat models. Though not significant, BDL+LLD4 group showed lower α-SMA expression compared to BDL group (58.40±14.271 vs. 63.20±9.16). However, administration of LLD4 did not give any significant effect on IL-6 expression. CONCLUSION: LLD4 reduces inflammation in models of obstructive jaundice by lowering the NF-κB and α-SMA expression. This indicates that LLD4 might be potential as an adjunct therapy for reducing morbidity in obstructive jaundice cases. KEYWORDS: obstructive jaundice, bile duct ligation, Lactococcus lactis D4, NF-κB, α-SMA, IL-6https://inabj.org/index.php/ibj/article/view/3384 |
| spellingShingle | Avit Suchitra Alvarino Alvarino Eryati Darwin Harnavi Harun Muhammad Iqbal Rivai Ade Sukma Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice Indonesian Biomedical Journal |
| title | Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice |
| title_full | Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice |
| title_fullStr | Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice |
| title_full_unstemmed | Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice |
| title_short | Lactococcus lactis D4 Decreases NF-κB and α-SMA in Rat Models of Obstructive Jaundice |
| title_sort | lactococcus lactis d4 decreases nf κb and α sma in rat models of obstructive jaundice |
| url | https://inabj.org/index.php/ibj/article/view/3384 |
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