Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection
Tuberculosis (TB) was, until SARS-CoV-2 pandemic, the leading cause of death by a single infectious agent contaminating over 10.6 million people with 1.6 million deaths in 2021 worldwide. Herein, we present a proof-of-principle strategy for detecting the recombinant protein CFP10:ESAT6 using an impe...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Sensors |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsens.2024.1512936/full |
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| author | Luisa Vogado Ribeiro Juliana Cancino-Bernardi Juliana Cancino-Bernardi Claudia do Amaral Razzino Thales Rafael Machado Marco A. M. Tuesta Valtencir Zucolotto |
| author_facet | Luisa Vogado Ribeiro Juliana Cancino-Bernardi Juliana Cancino-Bernardi Claudia do Amaral Razzino Thales Rafael Machado Marco A. M. Tuesta Valtencir Zucolotto |
| author_sort | Luisa Vogado Ribeiro |
| collection | DOAJ |
| description | Tuberculosis (TB) was, until SARS-CoV-2 pandemic, the leading cause of death by a single infectious agent contaminating over 10.6 million people with 1.6 million deaths in 2021 worldwide. Herein, we present a proof-of-principle strategy for detecting the recombinant protein CFP10:ESAT6 using an impedimetric immunosensor, which could aid in the diagnosis of tuberculosis. The immunosensor was developed using indium tin oxide electrodes modified by 3-aminopropyltrimethoxysilane monolayer to covalently immobilize anti-CFP10 antibodies. The protein interaction with the antibody recognition platform was directly monitored and measured by cyclic voltammetry and electrochemical impedance spectroscopy, respectively. After the analytical features optimization, a Langmuir isotherm response from 0.5 ng mL-1 to 50 ng mL-1 of pCFP10:ESAT6, limit of detection of 4.80 ng mL-1 and limit of quantification of 15.97 ng mL-1 were achieved, in a 4-hour assay time. Selectivity tests conducted in the presence of DENV NS1 and SARS-CoV-2 Spike proteins at a concentration of 20 ng mL-1, which is one-tenth of the concentration used to optimize pCFP10, indicate that the immunosensor is selective for pCFP10:ESAT6. Additionally, repeatability and reproducibility tests confirm that the immunosensor is suitable, accurate, and selective for detecting the CFP10:ESAT6 protein. The small sample volume required, and short testing time underscore the remarkable capabilities of this immunosensor and its potential for point-of-care screening and diagnostic aid applications. |
| format | Article |
| id | doaj-art-457e224a620f47bcb0c8c912f7e17c2b |
| institution | Kabale University |
| issn | 2673-5067 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sensors |
| spelling | doaj-art-457e224a620f47bcb0c8c912f7e17c2b2024-12-24T06:37:10ZengFrontiers Media S.A.Frontiers in Sensors2673-50672024-12-01510.3389/fsens.2024.15129361512936Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detectionLuisa Vogado Ribeiro0Juliana Cancino-Bernardi1Juliana Cancino-Bernardi2Claudia do Amaral Razzino3Thales Rafael Machado4Marco A. M. Tuesta5Valtencir Zucolotto6Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, BrazilNanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, BrazilChemistry Department, Laboratory in Bioanalytical of Nanosystems, Faculty of Philosophy, Science and Letters at Ribeirão Preto, University of São Paulo, Ribeirão Preto, BrazilNanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, BrazilNanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, BrazilNanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, BrazilNanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, BrazilTuberculosis (TB) was, until SARS-CoV-2 pandemic, the leading cause of death by a single infectious agent contaminating over 10.6 million people with 1.6 million deaths in 2021 worldwide. Herein, we present a proof-of-principle strategy for detecting the recombinant protein CFP10:ESAT6 using an impedimetric immunosensor, which could aid in the diagnosis of tuberculosis. The immunosensor was developed using indium tin oxide electrodes modified by 3-aminopropyltrimethoxysilane monolayer to covalently immobilize anti-CFP10 antibodies. The protein interaction with the antibody recognition platform was directly monitored and measured by cyclic voltammetry and electrochemical impedance spectroscopy, respectively. After the analytical features optimization, a Langmuir isotherm response from 0.5 ng mL-1 to 50 ng mL-1 of pCFP10:ESAT6, limit of detection of 4.80 ng mL-1 and limit of quantification of 15.97 ng mL-1 were achieved, in a 4-hour assay time. Selectivity tests conducted in the presence of DENV NS1 and SARS-CoV-2 Spike proteins at a concentration of 20 ng mL-1, which is one-tenth of the concentration used to optimize pCFP10, indicate that the immunosensor is selective for pCFP10:ESAT6. Additionally, repeatability and reproducibility tests confirm that the immunosensor is suitable, accurate, and selective for detecting the CFP10:ESAT6 protein. The small sample volume required, and short testing time underscore the remarkable capabilities of this immunosensor and its potential for point-of-care screening and diagnostic aid applications.https://www.frontiersin.org/articles/10.3389/fsens.2024.1512936/fullMycobacterium tuberculosisimmunosensorselectrochemical impedance spectroscopyimpedimetric biosensorsnanomedicine |
| spellingShingle | Luisa Vogado Ribeiro Juliana Cancino-Bernardi Juliana Cancino-Bernardi Claudia do Amaral Razzino Thales Rafael Machado Marco A. M. Tuesta Valtencir Zucolotto Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection Frontiers in Sensors Mycobacterium tuberculosis immunosensors electrochemical impedance spectroscopy impedimetric biosensors nanomedicine |
| title | Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection |
| title_full | Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection |
| title_fullStr | Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection |
| title_full_unstemmed | Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection |
| title_short | Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection |
| title_sort | exploring electrochemical impedance spectroscopy for the early diagnosis of mycobacterium tuberculosis using cfp10 esat6 protein detection |
| topic | Mycobacterium tuberculosis immunosensors electrochemical impedance spectroscopy impedimetric biosensors nanomedicine |
| url | https://www.frontiersin.org/articles/10.3389/fsens.2024.1512936/full |
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