Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens
This research examined the impact of chlorogenic acid (CGA) on laying performance, antioxidant capacity, egg quality, hepatic inflammation, mitochondrial function, and lipid metabolism in hens subjected to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative stres...
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2024-10-01
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| author | Haitong Zhao Zhuang Li Yue Sun Ming Yan Yingjie Wang Yurong Li Yeshun Zhang Mingkun Zhu |
| author_facet | Haitong Zhao Zhuang Li Yue Sun Ming Yan Yingjie Wang Yurong Li Yeshun Zhang Mingkun Zhu |
| author_sort | Haitong Zhao |
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| description | This research examined the impact of chlorogenic acid (CGA) on laying performance, antioxidant capacity, egg quality, hepatic inflammation, mitochondrial function, and lipid metabolism in hens subjected to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative stress (OS). Three hundred sixty healthy 43-wk-old Hy-Line brown hens were randomly assigned to six treatments: a basal diet + 0 (control and H<sub>2</sub>O<sub>2</sub>), 600 (600 mg/kg CGA and 600 mg/kg CGA + H<sub>2</sub>O<sub>2</sub>), and 800 (800 mg/kg CGA and 800 mg/kg CGA + H<sub>2</sub>O<sub>2</sub>) mg/kg CGA for 84 d. On the 64th and 78th days of the trial, hens in groups H<sub>2</sub>O<sub>2</sub>, 600 mg/kg CGA + H<sub>2</sub>O<sub>2</sub>, and 800 mg/kg CGA + H<sub>2</sub>O<sub>2</sub> were injected intraperitoneally with 10% H<sub>2</sub>O<sub>2</sub>. The results demonstrated that 600 and 800 mg/kg CGA significantly improved the egg production rate (EPR) and egg quality and reduced lipid peroxidation compared to the control group. The 800 mg/kg CGA showed greater improvements in the EPR and average egg weight (AEW) compared to the 600 mg/kg dose. Conversely, H<sub>2</sub>O<sub>2</sub> exposure significantly decreased the EPR, AEW, and egg quality and increased feed conversion rate and average daily feed intake. H<sub>2</sub>O<sub>2</sub> exposure significantly decreased serum T-AOC and increased serum MDA levels while reducing hepatic T-SOD, GSH-Px, and CAT activities. Meanwhile, H<sub>2</sub>O<sub>2</sub> exposure significantly elevated liver reactive oxygen species levels, pathological damage, and <i>NF-κB</i>, <i>TNFα</i>, and <i>IL-1β</i> gene expression. Additionally, H<sub>2</sub>O<sub>2</sub> treatment disrupted hepatocyte mitochondrial structure and significantly increased the expression of VDAC1 protein, and <i>IP3R</i>, <i>GRP75</i>, <i>MCU</i>, <i>Fis1</i>, and <i>MFF</i> genes, while downregulating the expression of MFN2 protein and <i>PGC1α</i> gene. Oil Red O staining demonstrated that H<sub>2</sub>O<sub>2</sub> induced significant lipid accumulation in hepatocytes. Concurrently, H<sub>2</sub>O<sub>2</sub> significantly increased serum triglycerides, total cholesterol, and liver triglycerides levels while decreasing serum hepatic lipase activity. This was primarily attributed to the significant upregulation of liver <i>SREBP1</i>, <i>FASN</i>, and <i>ACC</i> genes and the downregulation of the liver <i>CPT1</i> gene induced by H<sub>2</sub>O<sub>2</sub>. Furthermore, CGA pretreatment effectively prevented the degeneration in laying performance and egg quality, as well as OS, liver inflammation, pathological damage, and mitochondrial dysfunction induced by H<sub>2</sub>O<sub>2</sub>. CGA inhibited H<sub>2</sub>O<sub>2</sub>-induced hepatic lipid accumulation by upregulating fatty acid oxidation-related gene expression and downregulating fatty acid synthesis-related gene expression. These findings indicate that the dietary addition of 800 mg/kg of CGA is the optimum supplementation dose. CGA can enhance laying performance and egg quality while alleviating OS, hepatic inflammation, mitochondrial dysfunction, and lipid accumulation in H<sub>2</sub>O<sub>2</sub>-challenged laying hens. |
| format | Article |
| id | doaj-art-4576c8bcc2a84f518549c8a5cc141a19 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-10-01 |
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| series | Antioxidants |
| spelling | doaj-art-4576c8bcc2a84f518549c8a5cc141a192024-11-26T17:46:51ZengMDPI AGAntioxidants2076-39212024-10-011311130310.3390/antiox13111303Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying HensHaitong Zhao0Zhuang Li1Yue Sun2Ming Yan3Yingjie Wang4Yurong Li5Yeshun Zhang6Mingkun Zhu7Jiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaThis research examined the impact of chlorogenic acid (CGA) on laying performance, antioxidant capacity, egg quality, hepatic inflammation, mitochondrial function, and lipid metabolism in hens subjected to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative stress (OS). Three hundred sixty healthy 43-wk-old Hy-Line brown hens were randomly assigned to six treatments: a basal diet + 0 (control and H<sub>2</sub>O<sub>2</sub>), 600 (600 mg/kg CGA and 600 mg/kg CGA + H<sub>2</sub>O<sub>2</sub>), and 800 (800 mg/kg CGA and 800 mg/kg CGA + H<sub>2</sub>O<sub>2</sub>) mg/kg CGA for 84 d. On the 64th and 78th days of the trial, hens in groups H<sub>2</sub>O<sub>2</sub>, 600 mg/kg CGA + H<sub>2</sub>O<sub>2</sub>, and 800 mg/kg CGA + H<sub>2</sub>O<sub>2</sub> were injected intraperitoneally with 10% H<sub>2</sub>O<sub>2</sub>. The results demonstrated that 600 and 800 mg/kg CGA significantly improved the egg production rate (EPR) and egg quality and reduced lipid peroxidation compared to the control group. The 800 mg/kg CGA showed greater improvements in the EPR and average egg weight (AEW) compared to the 600 mg/kg dose. Conversely, H<sub>2</sub>O<sub>2</sub> exposure significantly decreased the EPR, AEW, and egg quality and increased feed conversion rate and average daily feed intake. H<sub>2</sub>O<sub>2</sub> exposure significantly decreased serum T-AOC and increased serum MDA levels while reducing hepatic T-SOD, GSH-Px, and CAT activities. Meanwhile, H<sub>2</sub>O<sub>2</sub> exposure significantly elevated liver reactive oxygen species levels, pathological damage, and <i>NF-κB</i>, <i>TNFα</i>, and <i>IL-1β</i> gene expression. Additionally, H<sub>2</sub>O<sub>2</sub> treatment disrupted hepatocyte mitochondrial structure and significantly increased the expression of VDAC1 protein, and <i>IP3R</i>, <i>GRP75</i>, <i>MCU</i>, <i>Fis1</i>, and <i>MFF</i> genes, while downregulating the expression of MFN2 protein and <i>PGC1α</i> gene. Oil Red O staining demonstrated that H<sub>2</sub>O<sub>2</sub> induced significant lipid accumulation in hepatocytes. Concurrently, H<sub>2</sub>O<sub>2</sub> significantly increased serum triglycerides, total cholesterol, and liver triglycerides levels while decreasing serum hepatic lipase activity. This was primarily attributed to the significant upregulation of liver <i>SREBP1</i>, <i>FASN</i>, and <i>ACC</i> genes and the downregulation of the liver <i>CPT1</i> gene induced by H<sub>2</sub>O<sub>2</sub>. Furthermore, CGA pretreatment effectively prevented the degeneration in laying performance and egg quality, as well as OS, liver inflammation, pathological damage, and mitochondrial dysfunction induced by H<sub>2</sub>O<sub>2</sub>. CGA inhibited H<sub>2</sub>O<sub>2</sub>-induced hepatic lipid accumulation by upregulating fatty acid oxidation-related gene expression and downregulating fatty acid synthesis-related gene expression. These findings indicate that the dietary addition of 800 mg/kg of CGA is the optimum supplementation dose. CGA can enhance laying performance and egg quality while alleviating OS, hepatic inflammation, mitochondrial dysfunction, and lipid accumulation in H<sub>2</sub>O<sub>2</sub>-challenged laying hens.https://www.mdpi.com/2076-3921/13/11/1303chlorogenic acidhydrogen peroxideoxidative stressliverlipid metabolismlaying hen |
| spellingShingle | Haitong Zhao Zhuang Li Yue Sun Ming Yan Yingjie Wang Yurong Li Yeshun Zhang Mingkun Zhu Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens Antioxidants chlorogenic acid hydrogen peroxide oxidative stress liver lipid metabolism laying hen |
| title | Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens |
| title_full | Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens |
| title_fullStr | Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens |
| title_full_unstemmed | Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens |
| title_short | Supplementation of Chlorogenic Acid Alleviates the Effects of H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens |
| title_sort | supplementation of chlorogenic acid alleviates the effects of h sub 2 sub o sub 2 sub induced oxidative stress on laying performance egg quality antioxidant capacity hepatic inflammation mitochondrial dysfunction and lipid accumulation in laying hens |
| topic | chlorogenic acid hydrogen peroxide oxidative stress liver lipid metabolism laying hen |
| url | https://www.mdpi.com/2076-3921/13/11/1303 |
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