Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers
Integrin α v β 3 plays great roles in tumor angiogenesis, invasion, and metastasis. We report here the noninvasive visualization of tumor integrin α v β 3 expression by using near-infrared fluorescence (NIRF) imaging of an IRDye800-labeled new cyclic RGD (arginine-glycine-aspartic acid) dimer with t...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2010-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2009.00032 |
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| _version_ | 1841563089373233152 |
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| author | Zhaofei Liu Shuanglong Liu Gang Niu Fan Wang Shuang Liu Xiaoyuan Chen |
| author_facet | Zhaofei Liu Shuanglong Liu Gang Niu Fan Wang Shuang Liu Xiaoyuan Chen |
| author_sort | Zhaofei Liu |
| collection | DOAJ |
| description | Integrin α v β 3 plays great roles in tumor angiogenesis, invasion, and metastasis. We report here the noninvasive visualization of tumor integrin α v β 3 expression by using near-infrared fluorescence (NIRF) imaging of an IRDye800-labeled new cyclic RGD (arginine-glycine-aspartic acid) dimer with tetra(ethylene glycol) (PEG 4 ) linkers (ie, E[PEG 4 -c(RGDfK)] 2 , PEG 4 = 15-amino-4,7,10,13-tetraoxapentadecanoic acid) in a U87MG tumor model. Fluorescent dye–labeled E[PEG 4 -c(RGDfK)] 2 were subjected to in vitro cell staining, in vivo NIRF imaging, ex vivo NIRF imaging, and histologic studies. The in vitro and in vivo characterization of dye-labeled E[PEG 4 -c(RGDfK)] 2 were compared with dye-labeled RGD dimer without PEG 4 linkers (namely, E[c(RGDfK)] 2 ). Both Cy5.5-E[PEG 4 -c(RGDfK)] 2 and Cy5.5-E[c(RGDfK)] 2 exhibited integrin α v β 3 binding specificity in a cell-staining experiment. In vivo NIRF imaging showed higher tumor accumulation and tumor to background contrast of IRDye800-E[PEG 4 -c(RGDfK)] 2 over IRDye800-E[c(RGDfK)] 2. The tumor integrin α v β 3 specificity of IRDye800-E[PEG 4 -c(RGDfK)] 2 was confirmed by successful inhibition of tumor uptake in the presence of an excess dose of c(RGDfK). Histologic examination revealed both tumor vasculature and tumor cell integrin α v β 3 binding of IRDye800-E[PEG 4 -c(RGDfK)] 2 in vivo. In summary, NIRF imaging with IRDye800-E[PEG 4 -c(RGDfK)] 2 offers an easy, fast, and low-cost way to detect and semiquantify tumor integrin α v β 3 expression in living subjects. |
| format | Article |
| id | doaj-art-450e76f5948e466f8d33703afd8b539c |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-450e76f5948e466f8d33703afd8b539c2025-01-03T00:12:14ZengSAGE PublishingMolecular Imaging1536-01212010-01-01910.2310/7290.2009.0003210.2310_7290.2009.00032Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) LinkersZhaofei LiuShuanglong LiuGang NiuFan WangShuang LiuXiaoyuan ChenIntegrin α v β 3 plays great roles in tumor angiogenesis, invasion, and metastasis. We report here the noninvasive visualization of tumor integrin α v β 3 expression by using near-infrared fluorescence (NIRF) imaging of an IRDye800-labeled new cyclic RGD (arginine-glycine-aspartic acid) dimer with tetra(ethylene glycol) (PEG 4 ) linkers (ie, E[PEG 4 -c(RGDfK)] 2 , PEG 4 = 15-amino-4,7,10,13-tetraoxapentadecanoic acid) in a U87MG tumor model. Fluorescent dye–labeled E[PEG 4 -c(RGDfK)] 2 were subjected to in vitro cell staining, in vivo NIRF imaging, ex vivo NIRF imaging, and histologic studies. The in vitro and in vivo characterization of dye-labeled E[PEG 4 -c(RGDfK)] 2 were compared with dye-labeled RGD dimer without PEG 4 linkers (namely, E[c(RGDfK)] 2 ). Both Cy5.5-E[PEG 4 -c(RGDfK)] 2 and Cy5.5-E[c(RGDfK)] 2 exhibited integrin α v β 3 binding specificity in a cell-staining experiment. In vivo NIRF imaging showed higher tumor accumulation and tumor to background contrast of IRDye800-E[PEG 4 -c(RGDfK)] 2 over IRDye800-E[c(RGDfK)] 2. The tumor integrin α v β 3 specificity of IRDye800-E[PEG 4 -c(RGDfK)] 2 was confirmed by successful inhibition of tumor uptake in the presence of an excess dose of c(RGDfK). Histologic examination revealed both tumor vasculature and tumor cell integrin α v β 3 binding of IRDye800-E[PEG 4 -c(RGDfK)] 2 in vivo. In summary, NIRF imaging with IRDye800-E[PEG 4 -c(RGDfK)] 2 offers an easy, fast, and low-cost way to detect and semiquantify tumor integrin α v β 3 expression in living subjects.https://doi.org/10.2310/7290.2009.00032 |
| spellingShingle | Zhaofei Liu Shuanglong Liu Gang Niu Fan Wang Shuang Liu Xiaoyuan Chen Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers Molecular Imaging |
| title | Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers |
| title_full | Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers |
| title_fullStr | Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers |
| title_full_unstemmed | Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers |
| title_short | Optical Imaging of Integrin αβ Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers |
| title_sort | optical imaging of integrin αβ expression with near infrared fluorescent rgd dimer with tetra ethylene glycol linkers |
| url | https://doi.org/10.2310/7290.2009.00032 |
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