Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut Honey

This study aimed to investigate the changes in physicochemical indexes and volatile components of gallnut honey, a traditional Chinese herbal honey during its ripening process. A series of physicochemical indexes, including water content and amylase value, were measured at different stages of maturi...

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Main Authors: Zao SHI, Xue YANG, Huiying LI, Shuxin ZHOU, Weizhong HU, Zhiqiang YI, Muying DU
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-08-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080368
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author Zao SHI
Xue YANG
Huiying LI
Shuxin ZHOU
Weizhong HU
Zhiqiang YI
Muying DU
author_facet Zao SHI
Xue YANG
Huiying LI
Shuxin ZHOU
Weizhong HU
Zhiqiang YI
Muying DU
author_sort Zao SHI
collection DOAJ
description This study aimed to investigate the changes in physicochemical indexes and volatile components of gallnut honey, a traditional Chinese herbal honey during its ripening process. A series of physicochemical indexes, including water content and amylase value, were measured at different stages of maturity. Additionally, the volatile components of gallnut honey were analyzed qualitatively and quantitatively using headspace solid-phase microextraction with gas chromatography-mass spectrometry (GC-MS), and the key aroma components were identified using relative odor activity value (ROAV). The results indicated that during the ripening process, the contents of protein, hydroxymethylfurfural, glucose, fructose, acidity and amylase value in gallnut honey increased, while the contents of water, electrical conductivity and sucrose decreased. The values of these indexes in mature gallnut honey were recorded as follows: 1.92±0.02 g/kg for protein, 0.67±0.03 mg/kg for hydroxymethylfurfural, 30.75±0.14 g/100 g for glucose, 35.90±1.08 g/100 g for fructose, 16.58±0.32 mL/kg for acidity, 20.59±1.25 mL/(g·h) for amylase value, 19.43±0.16 g/100 g for water content, 370.33±2.52 μs/cm for electrical conductivity and 2.62±0.12 g/100 g for sucrose. A total of 108 volatile components were identified across 11 categories, including acids, alcohols, aldehydes, and ketones, in gallnut honey at different maturation stages. The total amount of volatile components increased with longer brewing times, with aldehydes representing the highest proportion, ranging from 81.15% to 85.52%. Ketones were identified as the most diverse, comprising 24 species. Twelve key aroma components were selected from gallnut honey, 8 of which were common across all maturity stages: 2-methylbutyric acid, benzyl alcohol, benzaldehyde, phenylacetaldehyde, heptanal, nonanal, decanal and damastone. Notably, damastone, which imparted a fruity aroma, made the largest contribution to the overall fragrance of gallnut honey. The enhancement of floral and fruity aromas in gallnut honey was attributed to the interaction of these aroma components. This study provides theoretical guidance for the production and quality control of gallnut honey and offers research insights for the exploration of other distinctive traditional Chinese medicine honeys.
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publisher The editorial department of Science and Technology of Food Industry
record_format Article
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spelling doaj-art-44e69f168c704fde8fd32b47bb480e7f2025-08-20T04:03:21ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-08-01461529930910.13386/j.issn1002-0306.20240803682024080368-15Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut HoneyZao SHI0Xue YANG1Huiying LI2Shuxin ZHOU3Weizhong HU4Zhiqiang YI5Muying DU6College of Food Sciences, Southwest University, Chongqing 400715, ChinaCollege of Food Sciences, Southwest University, Chongqing 400715, ChinaCollege of Food Sciences, Southwest University, Chongqing 400715, ChinaCollege of Food Sciences, Southwest University, Chongqing 400715, ChinaCollege of Food Sciences, Southwest University, Chongqing 400715, ChinaCollege of Food Sciences, Southwest University, Chongqing 400715, ChinaCollege of Food Sciences, Southwest University, Chongqing 400715, ChinaThis study aimed to investigate the changes in physicochemical indexes and volatile components of gallnut honey, a traditional Chinese herbal honey during its ripening process. A series of physicochemical indexes, including water content and amylase value, were measured at different stages of maturity. Additionally, the volatile components of gallnut honey were analyzed qualitatively and quantitatively using headspace solid-phase microextraction with gas chromatography-mass spectrometry (GC-MS), and the key aroma components were identified using relative odor activity value (ROAV). The results indicated that during the ripening process, the contents of protein, hydroxymethylfurfural, glucose, fructose, acidity and amylase value in gallnut honey increased, while the contents of water, electrical conductivity and sucrose decreased. The values of these indexes in mature gallnut honey were recorded as follows: 1.92±0.02 g/kg for protein, 0.67±0.03 mg/kg for hydroxymethylfurfural, 30.75±0.14 g/100 g for glucose, 35.90±1.08 g/100 g for fructose, 16.58±0.32 mL/kg for acidity, 20.59±1.25 mL/(g·h) for amylase value, 19.43±0.16 g/100 g for water content, 370.33±2.52 μs/cm for electrical conductivity and 2.62±0.12 g/100 g for sucrose. A total of 108 volatile components were identified across 11 categories, including acids, alcohols, aldehydes, and ketones, in gallnut honey at different maturation stages. The total amount of volatile components increased with longer brewing times, with aldehydes representing the highest proportion, ranging from 81.15% to 85.52%. Ketones were identified as the most diverse, comprising 24 species. Twelve key aroma components were selected from gallnut honey, 8 of which were common across all maturity stages: 2-methylbutyric acid, benzyl alcohol, benzaldehyde, phenylacetaldehyde, heptanal, nonanal, decanal and damastone. Notably, damastone, which imparted a fruity aroma, made the largest contribution to the overall fragrance of gallnut honey. The enhancement of floral and fruity aromas in gallnut honey was attributed to the interaction of these aroma components. This study provides theoretical guidance for the production and quality control of gallnut honey and offers research insights for the exploration of other distinctive traditional Chinese medicine honeys.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080368gallnut honeyripening progressphysicochemical indexesheadspace solid-phase microextractionvolatile components
spellingShingle Zao SHI
Xue YANG
Huiying LI
Shuxin ZHOU
Weizhong HU
Zhiqiang YI
Muying DU
Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut Honey
Shipin gongye ke-ji
gallnut honey
ripening progress
physicochemical indexes
headspace solid-phase microextraction
volatile components
title Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut Honey
title_full Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut Honey
title_fullStr Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut Honey
title_full_unstemmed Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut Honey
title_short Changes in Physicochemical Indexes and Volatile Components during the Ripening Process of Gallnut Honey
title_sort changes in physicochemical indexes and volatile components during the ripening process of gallnut honey
topic gallnut honey
ripening progress
physicochemical indexes
headspace solid-phase microextraction
volatile components
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080368
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