“All-on-a-Tube” POCT of Salmonella in large-volume sample
Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive Salmonella detection in large-volume samples, where the entire bacterial detection progress from...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180424000941 |
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| author | Lei Wang Nana Jin Meixuan Li Jianhan Lin |
| author_facet | Lei Wang Nana Jin Meixuan Li Jianhan Lin |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive Salmonella detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 101 to 1.3 × 104 CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration. |
| format | Article |
| id | doaj-art-44811a9ae0cc4e9a891888de6b58d337 |
| institution | Kabale University |
| issn | 2214-1804 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-44811a9ae0cc4e9a891888de6b58d3372024-12-09T04:27:30ZengElsevierSensing and Bio-Sensing Research2214-18042024-12-0146100712“All-on-a-Tube” POCT of Salmonella in large-volume sampleLei Wang0Nana Jin1Meixuan Li2Jianhan Lin3Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, ChinaCorresponding author.; Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, ChinaPoint-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive Salmonella detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 101 to 1.3 × 104 CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration.http://www.sciencedirect.com/science/article/pii/S2214180424000941All-on-a-Tube colorimetric biosensorPoint-of-care testingLarge-volume sampleSalmonella detectionSmartphone image processing |
| spellingShingle | Lei Wang Nana Jin Meixuan Li Jianhan Lin “All-on-a-Tube” POCT of Salmonella in large-volume sample Sensing and Bio-Sensing Research All-on-a-Tube colorimetric biosensor Point-of-care testing Large-volume sample Salmonella detection Smartphone image processing |
| title | “All-on-a-Tube” POCT of Salmonella in large-volume sample |
| title_full | “All-on-a-Tube” POCT of Salmonella in large-volume sample |
| title_fullStr | “All-on-a-Tube” POCT of Salmonella in large-volume sample |
| title_full_unstemmed | “All-on-a-Tube” POCT of Salmonella in large-volume sample |
| title_short | “All-on-a-Tube” POCT of Salmonella in large-volume sample |
| title_sort | all on a tube poct of salmonella in large volume sample |
| topic | All-on-a-Tube colorimetric biosensor Point-of-care testing Large-volume sample Salmonella detection Smartphone image processing |
| url | http://www.sciencedirect.com/science/article/pii/S2214180424000941 |
| work_keys_str_mv | AT leiwang allonatubepoctofsalmonellainlargevolumesample AT nanajin allonatubepoctofsalmonellainlargevolumesample AT meixuanli allonatubepoctofsalmonellainlargevolumesample AT jianhanlin allonatubepoctofsalmonellainlargevolumesample |