Role of sclerostin in mastocytosis bone disease
Abstract Mastocytosis is a heterogeneous group of disorders, characterized by accumulation of clonal mast cells which can infiltrate several organs, most often spine (70%). The pathogenesis of mastocytosis bone disease is poorly understood. The main aim of the study was to investigate whether neopla...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-83851-0 |
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| author | Aneta Szudy-Szczyrek Radosław Mlak Dominika Pigoń-Zając Witold Krupski Marcin Mazurek Aleksandra Tomczak Karolina Chromik Aleksandra Górska Paweł Koźlik Adrian Juda Anna Kokoć Maciej Dubaj Tomasz Sacha Marek Niedoszytko Grzegorz Helbig Michał Szczyrek Justyna Szumiło Teresa Małecka-Massalska Marek Hus |
| author_facet | Aneta Szudy-Szczyrek Radosław Mlak Dominika Pigoń-Zając Witold Krupski Marcin Mazurek Aleksandra Tomczak Karolina Chromik Aleksandra Górska Paweł Koźlik Adrian Juda Anna Kokoć Maciej Dubaj Tomasz Sacha Marek Niedoszytko Grzegorz Helbig Michał Szczyrek Justyna Szumiło Teresa Małecka-Massalska Marek Hus |
| author_sort | Aneta Szudy-Szczyrek |
| collection | DOAJ |
| description | Abstract Mastocytosis is a heterogeneous group of disorders, characterized by accumulation of clonal mast cells which can infiltrate several organs, most often spine (70%). The pathogenesis of mastocytosis bone disease is poorly understood. The main aim of the study was to investigate whether neoplastic mast cells may be the source of sclerostin and whether there is an association between sclerostin and selected bone remodeling markers with mastocytosis related bone disease. We assessed sclerostin, bioactive sclerostin, and SOST gene expression in HMC-1.2 human mast cell culture supernatants and plasma of SM patients (n = 39). We showed that human mast cells can secrete sclerostin, and after their stimulation with IL-6, there is a significant increase in SOST gene expression. We observed significantly higher levels of sclerostin in patients diagnosed with more advanced disease. We observed a statistically significant correlation between concentations of sclerostin and its bioactive form and the concentration of alkaline phosphatase (ALP), and between sclerostin and interleukin-6 (IL-6). We observed that significantly higher sclerostin concentrations are present in patients with increased sclerosis of the spongy bone. Sclerostin may serve as a marker of more advanced disease and bone disease in mastocytosis. Further studies are justified to evaluate its role in mastocytosis. |
| format | Article |
| id | doaj-art-440e0b52720940c1b345a50fca75327d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-440e0b52720940c1b345a50fca75327d2025-01-05T12:13:23ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-024-83851-0Role of sclerostin in mastocytosis bone diseaseAneta Szudy-Szczyrek0Radosław Mlak1Dominika Pigoń-Zając2Witold Krupski3Marcin Mazurek4Aleksandra Tomczak5Karolina Chromik6Aleksandra Górska7Paweł Koźlik8Adrian Juda9Anna Kokoć10Maciej Dubaj11Tomasz Sacha12Marek Niedoszytko13Grzegorz Helbig14Michał Szczyrek15Justyna Szumiło16Teresa Małecka-Massalska17Marek Hus18Department of Haematooncology and Bone Marrow Transplantation, Medical University of LublinDepartment of Laboratory Diagnostics, Medical University of LublinDepartment of Human Physiology, Medical University of LublinDepartment of Medical Radiology, Medical University of LublinDepartment of Human Physiology, Medical University of LublinDepartment of Haematooncology and Bone Marrow Transplantation, Medical University of LublinDepartment of Hematology and Bone Marrow Transplantation, Medical University of Silesia in KatowiceDepartment of Pulmonology and Allergology, Medical University of GdańskChair of Hematology, Jagiellonian UniversityDepartment of Haematooncology and Bone Marrow Transplantation, Medical University of LublinDepartment of Haematooncology and Bone Marrow Transplantation, Medical University of LublinDepartment of Haematooncology and Bone Marrow Transplantation, Medical University of LublinChair of Hematology, Jagiellonian UniversityDepartment of Pulmonology and Allergology, Medical University of GdańskDepartment of Hematology and Bone Marrow Transplantation, Medical University of Silesia in KatowiceDepartment of Pneumonology, Oncology and Allergology, Medical University of LublinDepartment of Clinical Pathomorphology, Medical University of LublinDepartment of Human Physiology, Medical University of LublinDepartment of Haematooncology and Bone Marrow Transplantation, Medical University of LublinAbstract Mastocytosis is a heterogeneous group of disorders, characterized by accumulation of clonal mast cells which can infiltrate several organs, most often spine (70%). The pathogenesis of mastocytosis bone disease is poorly understood. The main aim of the study was to investigate whether neoplastic mast cells may be the source of sclerostin and whether there is an association between sclerostin and selected bone remodeling markers with mastocytosis related bone disease. We assessed sclerostin, bioactive sclerostin, and SOST gene expression in HMC-1.2 human mast cell culture supernatants and plasma of SM patients (n = 39). We showed that human mast cells can secrete sclerostin, and after their stimulation with IL-6, there is a significant increase in SOST gene expression. We observed significantly higher levels of sclerostin in patients diagnosed with more advanced disease. We observed a statistically significant correlation between concentations of sclerostin and its bioactive form and the concentration of alkaline phosphatase (ALP), and between sclerostin and interleukin-6 (IL-6). We observed that significantly higher sclerostin concentrations are present in patients with increased sclerosis of the spongy bone. Sclerostin may serve as a marker of more advanced disease and bone disease in mastocytosis. Further studies are justified to evaluate its role in mastocytosis.https://doi.org/10.1038/s41598-024-83851-0Bone remodelingBonesMastocytosisOsteolysisOsteosclerosisSclerostin |
| spellingShingle | Aneta Szudy-Szczyrek Radosław Mlak Dominika Pigoń-Zając Witold Krupski Marcin Mazurek Aleksandra Tomczak Karolina Chromik Aleksandra Górska Paweł Koźlik Adrian Juda Anna Kokoć Maciej Dubaj Tomasz Sacha Marek Niedoszytko Grzegorz Helbig Michał Szczyrek Justyna Szumiło Teresa Małecka-Massalska Marek Hus Role of sclerostin in mastocytosis bone disease Scientific Reports Bone remodeling Bones Mastocytosis Osteolysis Osteosclerosis Sclerostin |
| title | Role of sclerostin in mastocytosis bone disease |
| title_full | Role of sclerostin in mastocytosis bone disease |
| title_fullStr | Role of sclerostin in mastocytosis bone disease |
| title_full_unstemmed | Role of sclerostin in mastocytosis bone disease |
| title_short | Role of sclerostin in mastocytosis bone disease |
| title_sort | role of sclerostin in mastocytosis bone disease |
| topic | Bone remodeling Bones Mastocytosis Osteolysis Osteosclerosis Sclerostin |
| url | https://doi.org/10.1038/s41598-024-83851-0 |
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