Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study
Background: Atherosclerosis, a primary cause of coronary artery disease, involves chronic inflammatory arterial changes and contributes significantly to global vascular mortality. Genetic variations, especially single-nucleotide polymorphisms (SNPs) within microRNA (miRNA) machinery genes, impact mi...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Biomedical and Biotechnology Research Journal |
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| Online Access: | https://journals.lww.com/10.4103/bbrj.bbrj_256_24 |
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| author | Manar F. Atoum Dalya Alowaisy Ammar Ali Deeb |
| author_facet | Manar F. Atoum Dalya Alowaisy Ammar Ali Deeb |
| author_sort | Manar F. Atoum |
| collection | DOAJ |
| description | Background:
Atherosclerosis, a primary cause of coronary artery disease, involves chronic inflammatory arterial changes and contributes significantly to global vascular mortality. Genetic variations, especially single-nucleotide polymorphisms (SNPs) within microRNA (miRNA) machinery genes, impact miRNA biogenesis and play roles in atherosclerosis pathogenesis and progression. This study aimed to investigate the association between SNPs in DROSHA rs10719, DICER rs1057035, and XPO5 rs11077 as potential risk factors for atherosclerosis.
Methods:
The study included 100 individuals with clinically diagnosed atherosclerosis (mean age: 53 ± 7 years) and 100 control subjects (mean age: 52 ± 7 years). Genomic DNA was extracted and genotyped using real-time TaqMan allelic discrimination assays for DROSHA rs10719, DICER rs1057035, and XPO5 rs11077 SNPs.
Results:
The recessive model of DROSHA rs10719, particularly the GG genotype, showed a significant six-fold increased risk of atherosclerosis (adjusted odds ratio [AOR] 6.35, 95% confidence interval [CI] = 1.53–26.26, P = 0.0059). Conversely, the GG genotype of XPO5 rs11077 was more prevalent in controls (24% vs. 13%) and associated with reduced risk (OR = 0.39, 95% CI = 0.178–0.845, P = 0.019). In the recessive model, the GG genotype of XPO5 rs11077 remained protective (AOR = 0.14, 95% CI = 0.02–1.12, P = 0.04), consistent with findings from the log additive model (AOR = 0.27, 95% CI = 0.08–0.87, P = 0.016). Combinations of alleles involving DICER1 rs1057035, XPO5 rs11077, and DROSHA rs10719 (T-G-G, T-G-A, and C-T-A) were also associated with reduced risk of developing atherosclerosis (P < 0.05).
Conclusion:
This study highlights the association of specific SNPs in DROSHA and XPO5 genes with susceptibility to atherosclerosis, providing insights into the genetic factors that may contribute to the development and progression of these cardiovascular diseases. |
| format | Article |
| id | doaj-art-434f5853bc4743da83f196724ba4805a |
| institution | Kabale University |
| issn | 2588-9834 2588-9842 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Biomedical and Biotechnology Research Journal |
| spelling | doaj-art-434f5853bc4743da83f196724ba4805a2025-01-08T09:50:42ZengWolters Kluwer Medknow PublicationsBiomedical and Biotechnology Research Journal2588-98342588-98422024-12-018444745410.4103/bbrj.bbrj_256_24Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control StudyManar F. AtoumDalya AlowaisyAmmar Ali DeebBackground: Atherosclerosis, a primary cause of coronary artery disease, involves chronic inflammatory arterial changes and contributes significantly to global vascular mortality. Genetic variations, especially single-nucleotide polymorphisms (SNPs) within microRNA (miRNA) machinery genes, impact miRNA biogenesis and play roles in atherosclerosis pathogenesis and progression. This study aimed to investigate the association between SNPs in DROSHA rs10719, DICER rs1057035, and XPO5 rs11077 as potential risk factors for atherosclerosis. Methods: The study included 100 individuals with clinically diagnosed atherosclerosis (mean age: 53 ± 7 years) and 100 control subjects (mean age: 52 ± 7 years). Genomic DNA was extracted and genotyped using real-time TaqMan allelic discrimination assays for DROSHA rs10719, DICER rs1057035, and XPO5 rs11077 SNPs. Results: The recessive model of DROSHA rs10719, particularly the GG genotype, showed a significant six-fold increased risk of atherosclerosis (adjusted odds ratio [AOR] 6.35, 95% confidence interval [CI] = 1.53–26.26, P = 0.0059). Conversely, the GG genotype of XPO5 rs11077 was more prevalent in controls (24% vs. 13%) and associated with reduced risk (OR = 0.39, 95% CI = 0.178–0.845, P = 0.019). In the recessive model, the GG genotype of XPO5 rs11077 remained protective (AOR = 0.14, 95% CI = 0.02–1.12, P = 0.04), consistent with findings from the log additive model (AOR = 0.27, 95% CI = 0.08–0.87, P = 0.016). Combinations of alleles involving DICER1 rs1057035, XPO5 rs11077, and DROSHA rs10719 (T-G-G, T-G-A, and C-T-A) were also associated with reduced risk of developing atherosclerosis (P < 0.05). Conclusion: This study highlights the association of specific SNPs in DROSHA and XPO5 genes with susceptibility to atherosclerosis, providing insights into the genetic factors that may contribute to the development and progression of these cardiovascular diseases.https://journals.lww.com/10.4103/bbrj.bbrj_256_24atherosclerosiscoronary artery diseasemicrorna machinery genessingle-nucleotide polymorphisms |
| spellingShingle | Manar F. Atoum Dalya Alowaisy Ammar Ali Deeb Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study Biomedical and Biotechnology Research Journal atherosclerosis coronary artery disease microrna machinery genes single-nucleotide polymorphisms |
| title | Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study |
| title_full | Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study |
| title_fullStr | Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study |
| title_full_unstemmed | Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study |
| title_short | Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study |
| title_sort | analysis of microrna processing machinery gene drosha dicer1 and xpo5 variants association with atherosclerosis a case control study |
| topic | atherosclerosis coronary artery disease microrna machinery genes single-nucleotide polymorphisms |
| url | https://journals.lww.com/10.4103/bbrj.bbrj_256_24 |
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