Evaluation of nigella sativa's cold press oil as vaccine adjuvant
<b>Aim: </b>In order to identify an organic and plant based, less toxic, cheaper and more effective adjuvant, a cold pressed oil of Nigella sativa (CPNSO) and one of the essential components of Nigella sativa oil (NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant candidate...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2020-10-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1298 |
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| author | Uçkun Sait Uçan Aslı Sakmanoğlu Ali Uslu Zafer Sayın Ecem Oğlakçı Cansoy |
| author_facet | Uçkun Sait Uçan Aslı Sakmanoğlu Ali Uslu Zafer Sayın Ecem Oğlakçı Cansoy |
| author_sort | Uçkun Sait Uçan |
| collection | DOAJ |
| description | <b>Aim: </b>In order to identify an organic and plant based, less toxic, cheaper
and more effective adjuvant, a cold pressed oil of Nigella sativa
(CPNSO) and one of the essential components of Nigella sativa oil
(NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant
candidates.<p>
<b>Materials and Methods:</b> Adjuvant potentials of the both were measured
by examining specific antiibody titers to ovalbumin given in
presence of either of CPNSO or thymoquinone, in vivo. Such potentials
of thymoquinone alone (A1), CPNSO (A2), mCPNSO (experimentally
formed by addition of thymoquinone to CPNSO making up
mCPNSO that was contained 10% more thymoquinone than original
CPNSO did; A3) and Al(OH)3 (A4) in presence of ovalbumin in Swiss
albino mice (n=36, female). The effects were determined by measuring
anti-ovalbumin antibodies after immunization to ovalbumin by
home-made ELISA in mice.<p>
<b>Results:</b> Sera and conjugate were optimally diluted 1/200 and
1/40.000, respectively. Mean OD values at 450 nm of the groups
control (A0), A1, A2, A3 and A4 were 0,043 (± 0,002), 0,668 (±
0,074), 0,644 (± 0,018), 0,675 (± 0,066), and 0,745 (± 0,09), respectively.<p>
<b>Conclusion:</b> By comparison control, all three (A1, A2, A3) of the test
formulations were found to be as effective as commercial (A4) formulation
in triggering humoral response to ovalbumin (p > 0,05).
Therefore, each of Nigella sativa based adjuvant candidates has an
alternative potential to Al(OH)3 in the mouse. |
| format | Article |
| id | doaj-art-4335c44ed5f943e19a3f536349728b51 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-4335c44ed5f943e19a3f536349728b512025-01-03T01:36:24ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532020-10-013631992031298Evaluation of nigella sativa's cold press oil as vaccine adjuvantUçkun Sait UçanAslı SakmanoğluAli UsluZafer SayınEcem Oğlakçı Cansoy<b>Aim: </b>In order to identify an organic and plant based, less toxic, cheaper and more effective adjuvant, a cold pressed oil of Nigella sativa (CPNSO) and one of the essential components of Nigella sativa oil (NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant candidates.<p> <b>Materials and Methods:</b> Adjuvant potentials of the both were measured by examining specific antiibody titers to ovalbumin given in presence of either of CPNSO or thymoquinone, in vivo. Such potentials of thymoquinone alone (A1), CPNSO (A2), mCPNSO (experimentally formed by addition of thymoquinone to CPNSO making up mCPNSO that was contained 10% more thymoquinone than original CPNSO did; A3) and Al(OH)3 (A4) in presence of ovalbumin in Swiss albino mice (n=36, female). The effects were determined by measuring anti-ovalbumin antibodies after immunization to ovalbumin by home-made ELISA in mice.<p> <b>Results:</b> Sera and conjugate were optimally diluted 1/200 and 1/40.000, respectively. Mean OD values at 450 nm of the groups control (A0), A1, A2, A3 and A4 were 0,043 (± 0,002), 0,668 (± 0,074), 0,644 (± 0,018), 0,675 (± 0,066), and 0,745 (± 0,09), respectively.<p> <b>Conclusion:</b> By comparison control, all three (A1, A2, A3) of the test formulations were found to be as effective as commercial (A4) formulation in triggering humoral response to ovalbumin (p > 0,05). Therefore, each of Nigella sativa based adjuvant candidates has an alternative potential to Al(OH)3 in the mouse.http://eurasianjvetsci.org/pdf.php3?id=1298thymoquinoneadjuvantovalbuminmouse |
| spellingShingle | Uçkun Sait Uçan Aslı Sakmanoğlu Ali Uslu Zafer Sayın Ecem Oğlakçı Cansoy Evaluation of nigella sativa's cold press oil as vaccine adjuvant Eurasian Journal of Veterinary Sciences thymoquinone adjuvant ovalbumin mouse |
| title | Evaluation of nigella sativa's cold press oil as vaccine adjuvant |
| title_full | Evaluation of nigella sativa's cold press oil as vaccine adjuvant |
| title_fullStr | Evaluation of nigella sativa's cold press oil as vaccine adjuvant |
| title_full_unstemmed | Evaluation of nigella sativa's cold press oil as vaccine adjuvant |
| title_short | Evaluation of nigella sativa's cold press oil as vaccine adjuvant |
| title_sort | evaluation of nigella sativa s cold press oil as vaccine adjuvant |
| topic | thymoquinone adjuvant ovalbumin mouse |
| url | http://eurasianjvetsci.org/pdf.php3?id=1298 |
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