Rheological comparison of sputum and reconstituted airway epithelium mucus
Abstract Pulmonary mucus serves as a crucial protective barrier in the respiratory tract, defending against pathogens and contributing to effective clearance mechanisms. In Muco Obstructive Pulmonary Diseases (MOPD), abnormal rheological properties lead to highly viscous mucus, fostering chronic inf...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-80932-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559492622286848 |
|---|---|
| author | Lydia Esteban Enjuto Vassylia Taty Poaty Mendy Bouveret Huang Song Samuel Constant Jérémy Patarin |
| author_facet | Lydia Esteban Enjuto Vassylia Taty Poaty Mendy Bouveret Huang Song Samuel Constant Jérémy Patarin |
| author_sort | Lydia Esteban Enjuto |
| collection | DOAJ |
| description | Abstract Pulmonary mucus serves as a crucial protective barrier in the respiratory tract, defending against pathogens and contributing to effective clearance mechanisms. In Muco Obstructive Pulmonary Diseases (MOPD), abnormal rheological properties lead to highly viscous mucus, fostering chronic infections and exacerbations. While prior research has linked mucus viscoelasticity to its mucin content, the variability in MOPD patients implies the involvement of other factors. To isolate these effects, mucus produced by epithelia reconstituted in vitro serves as a powerful versatile model for mucin research. This study characterises the rheology of mucus collected from Air-Liquid Interface (ALI) cultures and compares it to sputum samples from MOPD patients, demonstrating that macrorheology with cone-plate geometries is a reproducible method for analysing small mucus quantities from ALI cultures. While sputum samples exhibit similarities in rigidity with ALI mucus, they also display structural differences and variations in their response to substantial deformations. The study highlights the importance of understanding mucus behaviour under large deformations, emphasising the role of ALI cultures as a controlled environment for conducting detailed studies. |
| format | Article |
| id | doaj-art-4238c1a942274ddb8546ede53689dee3 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4238c1a942274ddb8546ede53689dee32025-01-05T12:26:10ZengNature PortfolioScientific Reports2045-23222024-12-011411810.1038/s41598-024-80932-yRheological comparison of sputum and reconstituted airway epithelium mucusLydia Esteban Enjuto0Vassylia Taty Poaty1Mendy Bouveret2Huang Song3Samuel Constant4Jérémy Patarin5RheonovaRheonovaEpithelixEpithelixEpithelixRheonovaAbstract Pulmonary mucus serves as a crucial protective barrier in the respiratory tract, defending against pathogens and contributing to effective clearance mechanisms. In Muco Obstructive Pulmonary Diseases (MOPD), abnormal rheological properties lead to highly viscous mucus, fostering chronic infections and exacerbations. While prior research has linked mucus viscoelasticity to its mucin content, the variability in MOPD patients implies the involvement of other factors. To isolate these effects, mucus produced by epithelia reconstituted in vitro serves as a powerful versatile model for mucin research. This study characterises the rheology of mucus collected from Air-Liquid Interface (ALI) cultures and compares it to sputum samples from MOPD patients, demonstrating that macrorheology with cone-plate geometries is a reproducible method for analysing small mucus quantities from ALI cultures. While sputum samples exhibit similarities in rigidity with ALI mucus, they also display structural differences and variations in their response to substantial deformations. The study highlights the importance of understanding mucus behaviour under large deformations, emphasising the role of ALI cultures as a controlled environment for conducting detailed studies.https://doi.org/10.1038/s41598-024-80932-y |
| spellingShingle | Lydia Esteban Enjuto Vassylia Taty Poaty Mendy Bouveret Huang Song Samuel Constant Jérémy Patarin Rheological comparison of sputum and reconstituted airway epithelium mucus Scientific Reports |
| title | Rheological comparison of sputum and reconstituted airway epithelium mucus |
| title_full | Rheological comparison of sputum and reconstituted airway epithelium mucus |
| title_fullStr | Rheological comparison of sputum and reconstituted airway epithelium mucus |
| title_full_unstemmed | Rheological comparison of sputum and reconstituted airway epithelium mucus |
| title_short | Rheological comparison of sputum and reconstituted airway epithelium mucus |
| title_sort | rheological comparison of sputum and reconstituted airway epithelium mucus |
| url | https://doi.org/10.1038/s41598-024-80932-y |
| work_keys_str_mv | AT lydiaestebanenjuto rheologicalcomparisonofsputumandreconstitutedairwayepitheliummucus AT vassyliatatypoaty rheologicalcomparisonofsputumandreconstitutedairwayepitheliummucus AT mendybouveret rheologicalcomparisonofsputumandreconstitutedairwayepitheliummucus AT huangsong rheologicalcomparisonofsputumandreconstitutedairwayepitheliummucus AT samuelconstant rheologicalcomparisonofsputumandreconstitutedairwayepitheliummucus AT jeremypatarin rheologicalcomparisonofsputumandreconstitutedairwayepitheliummucus |