Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad City
Staphylococcus aureus bacteria is cause of dangerous food poisoning through contamination of raw milk; therefore, this study aimed to determine the prevalence of S. aureus in raw milk sold in local markets of Baghdad city and the antibacterial activity of chitosan, trisodium citrate and acetic acid...
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| Format: | Article |
| Language: | Arabic |
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College of Veterinary Medicine, University of Fallujah
2024-02-01
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| Series: | مجلة الانبار للعلوم البيطرية |
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| Online Access: | https://www.anbarjvs.edu.iq/?mno=198641 |
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| author | Zina Saab Khudhir Zina Saab Khudhir |
| author_facet | Zina Saab Khudhir Zina Saab Khudhir |
| author_sort | Zina Saab Khudhir |
| collection | DOAJ |
| description | Staphylococcus aureus bacteria is cause of dangerous food poisoning through contamination of raw milk; therefore, this study aimed to determine the prevalence of S. aureus in raw milk sold in local markets of Baghdad city and the antibacterial activity of chitosan, trisodium citrate and acetic acid against the pathogenic bacteria, the antibacterial susceptibility profile of isolates was performed by using (13) antibacterial agents using Kirby–Bauer disc diffusion technique. the results showed that (3) samples out of 25 samples (12%) were isolated from raw milk, all isolates were positive to the experimental tests characterized as S. aureus depending on the cultural, biochemical, serological and molecular examinations. The PCR technique used to verify the positive isolates by targeting the (nuc) gene, the molecular size (181 bp), the antibacterial activity was done by broth and agar dilution method with detection the bacterial counts. the Initial counting (mean) log CFU/ ml of isolates was 4.623 CFU/ml. The minimum inhibitory concentration of chitosan and acetic acid against Staphylococcus aureus by macro-broth dilution method was 20and 1,25 mg /ml respectively, while trisodium citrate had no inhibitory activity against the exanimated bacteria. The antibiotic susceptibility profile for positive isolates showed that all isolates were resistant to (ampicillin, methicillin, Cefixime and Cefoxitin),in conclusion comparing the effects of the three antibacterial agents against S. aureus causes contamination of cow raw milk sold in Baghdad city, 1.25 mg/ml of the acetic acid was found effective with higher antibacterial activity, followed by chitosan 2 mg /ml, while trisodium citrate showed no antibacterial effect. [AJVS 2024; 17(2.000): 80-94] |
| format | Article |
| id | doaj-art-4238af5f94c54903b0140d741e30ccf6 |
| institution | Kabale University |
| issn | 1999-6527 2707-0603 |
| language | Arabic |
| publishDate | 2024-02-01 |
| publisher | College of Veterinary Medicine, University of Fallujah |
| record_format | Article |
| series | مجلة الانبار للعلوم البيطرية |
| spelling | doaj-art-4238af5f94c54903b0140d741e30ccf62024-12-14T06:44:49ZaraCollege of Veterinary Medicine, University of Fallujahمجلة الانبار للعلوم البيطرية1999-65272707-06032024-02-01172809410.37940/AJVS.2024.17.2.11198641Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad CityZina Saab Khudhir0Zina Saab Khudhir1Professor in university of Baghdad / Iraq Professor in university of Baghdad / IraqStaphylococcus aureus bacteria is cause of dangerous food poisoning through contamination of raw milk; therefore, this study aimed to determine the prevalence of S. aureus in raw milk sold in local markets of Baghdad city and the antibacterial activity of chitosan, trisodium citrate and acetic acid against the pathogenic bacteria, the antibacterial susceptibility profile of isolates was performed by using (13) antibacterial agents using Kirby–Bauer disc diffusion technique. the results showed that (3) samples out of 25 samples (12%) were isolated from raw milk, all isolates were positive to the experimental tests characterized as S. aureus depending on the cultural, biochemical, serological and molecular examinations. The PCR technique used to verify the positive isolates by targeting the (nuc) gene, the molecular size (181 bp), the antibacterial activity was done by broth and agar dilution method with detection the bacterial counts. the Initial counting (mean) log CFU/ ml of isolates was 4.623 CFU/ml. The minimum inhibitory concentration of chitosan and acetic acid against Staphylococcus aureus by macro-broth dilution method was 20and 1,25 mg /ml respectively, while trisodium citrate had no inhibitory activity against the exanimated bacteria. The antibiotic susceptibility profile for positive isolates showed that all isolates were resistant to (ampicillin, methicillin, Cefixime and Cefoxitin),in conclusion comparing the effects of the three antibacterial agents against S. aureus causes contamination of cow raw milk sold in Baghdad city, 1.25 mg/ml of the acetic acid was found effective with higher antibacterial activity, followed by chitosan 2 mg /ml, while trisodium citrate showed no antibacterial effect. [AJVS 2024; 17(2.000): 80-94]https://www.anbarjvs.edu.iq/?mno=198641chitosan; staphylococcus aureus; trisodium citrateacetic acidraw milk |
| spellingShingle | Zina Saab Khudhir Zina Saab Khudhir Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad City مجلة الانبار للعلوم البيطرية chitosan; staphylococcus aureus; trisodium citrate acetic acid raw milk |
| title | Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad City |
| title_full | Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad City |
| title_fullStr | Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad City |
| title_full_unstemmed | Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad City |
| title_short | Antibacterial Activity of Chitosan, Trisodium Citrate and Acetic Acid Against Staphylococcus Aureus Bacteria Isolated From Raw Milk In Baghdad City |
| title_sort | antibacterial activity of chitosan trisodium citrate and acetic acid against staphylococcus aureus bacteria isolated from raw milk in baghdad city |
| topic | chitosan; staphylococcus aureus; trisodium citrate acetic acid raw milk |
| url | https://www.anbarjvs.edu.iq/?mno=198641 |
| work_keys_str_mv | AT zinasaabkhudhir antibacterialactivityofchitosantrisodiumcitrateandaceticacidagainststaphylococcusaureusbacteriaisolatedfromrawmilkinbaghdadcity AT zinasaabkhudhir antibacterialactivityofchitosantrisodiumcitrateandaceticacidagainststaphylococcusaureusbacteriaisolatedfromrawmilkinbaghdadcity |