Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro
Background and Objectives: Medicinal plants are the primary treatment for many infectious and non-infectious diseases. In this study, we evaluated the antiviral activity of Ferula assa-feotida against herpes simplex viruses 1 and 2, and compared it with the antiviral drug acyclovir. Materials and M...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4559 |
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| author | Javad Charostad Tahere Navidfar Masoumeh Kiani Paul Schinitzler Akram Astani |
| author_facet | Javad Charostad Tahere Navidfar Masoumeh Kiani Paul Schinitzler Akram Astani |
| author_sort | Javad Charostad |
| collection | DOAJ |
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Background and Objectives: Medicinal plants are the primary treatment for many infectious and non-infectious diseases. In this study, we evaluated the antiviral activity of Ferula assa-feotida against herpes simplex viruses 1 and 2, and compared it with the antiviral drug acyclovir.
Materials and Methods: In our experimental study, Ferula assa-feotida was dissolved in DMSO, then diluted in DMEM medium. Acyclovir was used at a concentration of 100 μM in all procedures. The antiherpetic activity and Antiviral activity of Ferula were evaluated in Vero cells (African green monkey kidney cells) by using the plaque reduction assay.
Results: Inhibitory concentrations of 50% (IC50) of Ferula assa-feotida for HSV-1 and HSV-2 were determined at 0.00025% and 0.00015%, respectively. Ferula was introduced at various stages of viral infection and significantly inhibited HSV-1 and HSV-2 infectivity by > 95.5% and 89%, respectively, when virus was pre-treated before addition to the cells. No HSV-1 or HSV-2 activity was detected in cells treated prior to and following viral infection.
Conclusion: These results indicate that Ferula assa-feotida demonstrates antiherpetic activity in the early phase of viral infection and could be used as potential antiviral agent.
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| format | Article |
| id | doaj-art-4233295fa0ed49a5bf1ed1184459e241 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-4233295fa0ed49a5bf1ed1184459e2412025-01-06T08:38:45ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-12-0116610.18502/ijm.v16i6.17257Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitroJavad Charostad0Tahere Navidfar1Masoumeh Kiani2Paul Schinitzler3Akram Astani4Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Basic Sciences, Shoushtar Faculty of Medical Sciences, Shushtar University of Medical and Science, Shushtar, IranDepartment of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, GermanyDepartment of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Background and Objectives: Medicinal plants are the primary treatment for many infectious and non-infectious diseases. In this study, we evaluated the antiviral activity of Ferula assa-feotida against herpes simplex viruses 1 and 2, and compared it with the antiviral drug acyclovir. Materials and Methods: In our experimental study, Ferula assa-feotida was dissolved in DMSO, then diluted in DMEM medium. Acyclovir was used at a concentration of 100 μM in all procedures. The antiherpetic activity and Antiviral activity of Ferula were evaluated in Vero cells (African green monkey kidney cells) by using the plaque reduction assay. Results: Inhibitory concentrations of 50% (IC50) of Ferula assa-feotida for HSV-1 and HSV-2 were determined at 0.00025% and 0.00015%, respectively. Ferula was introduced at various stages of viral infection and significantly inhibited HSV-1 and HSV-2 infectivity by > 95.5% and 89%, respectively, when virus was pre-treated before addition to the cells. No HSV-1 or HSV-2 activity was detected in cells treated prior to and following viral infection. Conclusion: These results indicate that Ferula assa-feotida demonstrates antiherpetic activity in the early phase of viral infection and could be used as potential antiviral agent. https://ijm.tums.ac.ir/index.php/ijm/article/view/4559Ferula assa-foetida;Antiviral;Herpes simplex virus type 1;Herpes simplex virus type 2 |
| spellingShingle | Javad Charostad Tahere Navidfar Masoumeh Kiani Paul Schinitzler Akram Astani Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro Iranian Journal of Microbiology Ferula assa-foetida; Antiviral; Herpes simplex virus type 1; Herpes simplex virus type 2 |
| title | Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro |
| title_full | Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro |
| title_fullStr | Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro |
| title_full_unstemmed | Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro |
| title_short | Antiviral activity of Ferula assa-feotida on HSV-1, 2 in vitro |
| title_sort | antiviral activity of ferula assa feotida on hsv 1 2 in vitro |
| topic | Ferula assa-foetida; Antiviral; Herpes simplex virus type 1; Herpes simplex virus type 2 |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4559 |
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