Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects
Abstract Background Exposure to standard petrodiesel exhaust is linked to adverse health effects. Moreover, there is a mounting request to replace fossil-based fuels with renewable and sustainable alternatives and, therefore, rapeseed methyl ester (RME) and other biofuels have been introduced. Howev...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
|
| Series: | Particle and Fibre Toxicology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12989-024-00614-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846137492080689152 |
|---|---|
| author | Thomas Sandström Jenny A. Bosson Ala Muala Mikael Kabéle Jamshid Pourazar Christoffer Boman Gregory Rankin Ian S. Mudway Anders Blomberg Maria Friberg |
| author_facet | Thomas Sandström Jenny A. Bosson Ala Muala Mikael Kabéle Jamshid Pourazar Christoffer Boman Gregory Rankin Ian S. Mudway Anders Blomberg Maria Friberg |
| author_sort | Thomas Sandström |
| collection | DOAJ |
| description | Abstract Background Exposure to standard petrodiesel exhaust is linked to adverse health effects. Moreover, there is a mounting request to replace fossil-based fuels with renewable and sustainable alternatives and, therefore, rapeseed methyl ester (RME) and other biofuels have been introduced. However, recent toxicological research has indicated that biodiesel exhaust may also induce adverse health-related events. Aim To determine whether exposure to 100% RME biodiesel (BD100) exhaust would cause an acute airway neutrophilic recruitment in humans. Methods Fourteen healthy subjects underwent exposure to diluted BD100 exhaust and filtered air for 1-h, in a blinded, random fashion. Bronchoscopy with endobronchial mucosal biopsies, bronchial wash (BW) and bronchoalveolar lavage (BAL) was performed six hours after exposure. Differential cell counts and inflammatory markers were determined in the supernatant and biopsies were stained immunohistochemically. Results Compared with filtered air, BD100 exhaust exposure increased bronchial mucosal endothelial P-selectin adhesion molecule expression, as well as neutrophil, mast cell and CD68 + macrophage numbers. An increased influx of neutrophils and machrophages was also seen in BW. Conclusion Exposure to biodiesel exhaust was associated with an acute airway inflammation that appeared similar to preceding petrodiesel exposure studies. The present findings, together with the recently reported adverse cardiovascular effects after similar biodiesel exposure, indicate that biodiesel is not free of toxicity and may affect human health. |
| format | Article |
| id | doaj-art-41f5eb297fb1438eaf7515078f2b6e6e |
| institution | Kabale University |
| issn | 1743-8977 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj-art-41f5eb297fb1438eaf7515078f2b6e6e2024-12-08T12:07:03ZengBMCParticle and Fibre Toxicology1743-89772024-12-0121111210.1186/s12989-024-00614-5Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjectsThomas Sandström0Jenny A. Bosson1Ala Muala2Mikael Kabéle3Jamshid Pourazar4Christoffer Boman5Gregory Rankin6Ian S. Mudway7Anders Blomberg8Maria Friberg9Department of Public Health and Clinical Medicine, Umeå UniversityDepartment of Public Health and Clinical Medicine, Umeå UniversityDepartment of Public Health and Clinical Medicine, Umeå UniversityDepartment of Public Health and Clinical Medicine, Umeå UniversityDepartment of Public Health and Clinical Medicine, Umeå UniversityThermochemical Energy Conversion Laboratory, Department of Applied Physics and Electronics, Umeå UniversityDepartment of Public Health and Clinical Medicine, Umeå UniversityNIHR Health Protection Research Unit in Environmental Exposures and Health, MRC-PHE Centre for Environment and Health, Imperial College LondonDepartment of Public Health and Clinical Medicine, Umeå UniversityDepartment of Public Health and Clinical Medicine, Umeå UniversityAbstract Background Exposure to standard petrodiesel exhaust is linked to adverse health effects. Moreover, there is a mounting request to replace fossil-based fuels with renewable and sustainable alternatives and, therefore, rapeseed methyl ester (RME) and other biofuels have been introduced. However, recent toxicological research has indicated that biodiesel exhaust may also induce adverse health-related events. Aim To determine whether exposure to 100% RME biodiesel (BD100) exhaust would cause an acute airway neutrophilic recruitment in humans. Methods Fourteen healthy subjects underwent exposure to diluted BD100 exhaust and filtered air for 1-h, in a blinded, random fashion. Bronchoscopy with endobronchial mucosal biopsies, bronchial wash (BW) and bronchoalveolar lavage (BAL) was performed six hours after exposure. Differential cell counts and inflammatory markers were determined in the supernatant and biopsies were stained immunohistochemically. Results Compared with filtered air, BD100 exhaust exposure increased bronchial mucosal endothelial P-selectin adhesion molecule expression, as well as neutrophil, mast cell and CD68 + macrophage numbers. An increased influx of neutrophils and machrophages was also seen in BW. Conclusion Exposure to biodiesel exhaust was associated with an acute airway inflammation that appeared similar to preceding petrodiesel exposure studies. The present findings, together with the recently reported adverse cardiovascular effects after similar biodiesel exposure, indicate that biodiesel is not free of toxicity and may affect human health.https://doi.org/10.1186/s12989-024-00614-5Air pollutionLungChamber exposureBiodieselRenewable fuelBronchoscopy |
| spellingShingle | Thomas Sandström Jenny A. Bosson Ala Muala Mikael Kabéle Jamshid Pourazar Christoffer Boman Gregory Rankin Ian S. Mudway Anders Blomberg Maria Friberg Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects Particle and Fibre Toxicology Air pollution Lung Chamber exposure Biodiesel Renewable fuel Bronchoscopy |
| title | Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects |
| title_full | Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects |
| title_fullStr | Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects |
| title_full_unstemmed | Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects |
| title_short | Acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects |
| title_sort | acute airway inflammation following controlled biodiesel exhaust exposure in healthy subjects |
| topic | Air pollution Lung Chamber exposure Biodiesel Renewable fuel Bronchoscopy |
| url | https://doi.org/10.1186/s12989-024-00614-5 |
| work_keys_str_mv | AT thomassandstrom acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT jennyabosson acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT alamuala acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT mikaelkabele acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT jamshidpourazar acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT christofferboman acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT gregoryrankin acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT iansmudway acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT andersblomberg acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects AT mariafriberg acuteairwayinflammationfollowingcontrolledbiodieselexhaustexposureinhealthysubjects |