Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis
BackgroundSigmoid colon cancer with spinal metastases is rare in distant metastasis. In addition, the prognosis of colon cancer patients with spinal metastases is extremely poor. In order to find effective therapeutic agents, we need to know the biological characteristics of such patients from relat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1510264/full |
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| author | Jingyao Chen Jingyao Chen Shumin Cheng Liang Gu Liang Gu Zhangsen Huang Zhangsen Huang Changhua Zhang Changhua Zhang Chunhui Sun Chunhui Sun Chunhui Sun Songyao Chen Songyao Chen |
| author_facet | Jingyao Chen Jingyao Chen Shumin Cheng Liang Gu Liang Gu Zhangsen Huang Zhangsen Huang Changhua Zhang Changhua Zhang Chunhui Sun Chunhui Sun Chunhui Sun Songyao Chen Songyao Chen |
| author_sort | Jingyao Chen |
| collection | DOAJ |
| description | BackgroundSigmoid colon cancer with spinal metastases is rare in distant metastasis. In addition, the prognosis of colon cancer patients with spinal metastases is extremely poor. In order to find effective therapeutic agents, we need to know the biological characteristics of such patients from related models.MethodsWe collected sigmoid colon cancer tissue from a young female subject who was diagnosed with sigmoid colon cancer with multiple spinal metastases. We successfully established a sigmoid colon cancer organoid using this tissue and investigated drug screening in the patient. HE staining, immunohistochemistry, and DNA sequencing were utilized to compare the biological characteristics between the original tumor and the organoid. Furthermore, we investigated the drug screening of the sigmoid colon cancer organoid in vitro.ResultsA colon cancer organoid from sigmoid colon cancer with spinal metastases was successfully established. The organoid culture maintained the morphological features, histological features, and genomic landscape of the corresponding sigmoid colon cancer cells. Moreover, we performed drug screening tests to evaluate the effects of chemotherapeutic drugs and targeted drugs.ConclusionThe sigmoid colon cancer organoid with spinal metastases was a favorable preclinical model to explore the clinicopathologic characteristics of colon cancer patients with spinal metastases. |
| format | Article |
| id | doaj-art-41bde8757f23472e99447a982f7a14f4 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-41bde8757f23472e99447a982f7a14f42025-01-03T06:46:58ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011210.3389/fcell.2024.15102641510264Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasisJingyao Chen0Jingyao Chen1Shumin Cheng2Liang Gu3Liang Gu4Zhangsen Huang5Zhangsen Huang6Changhua Zhang7Changhua Zhang8Chunhui Sun9Chunhui Sun10Chunhui Sun11Songyao Chen12Songyao Chen13Digestive Diseases Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaScientific Research Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDigestive Diseases Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDigestive Diseases Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDigestive Diseases Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDigestive Diseases Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaScientific Research Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDigestive Diseases Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaBackgroundSigmoid colon cancer with spinal metastases is rare in distant metastasis. In addition, the prognosis of colon cancer patients with spinal metastases is extremely poor. In order to find effective therapeutic agents, we need to know the biological characteristics of such patients from related models.MethodsWe collected sigmoid colon cancer tissue from a young female subject who was diagnosed with sigmoid colon cancer with multiple spinal metastases. We successfully established a sigmoid colon cancer organoid using this tissue and investigated drug screening in the patient. HE staining, immunohistochemistry, and DNA sequencing were utilized to compare the biological characteristics between the original tumor and the organoid. Furthermore, we investigated the drug screening of the sigmoid colon cancer organoid in vitro.ResultsA colon cancer organoid from sigmoid colon cancer with spinal metastases was successfully established. The organoid culture maintained the morphological features, histological features, and genomic landscape of the corresponding sigmoid colon cancer cells. Moreover, we performed drug screening tests to evaluate the effects of chemotherapeutic drugs and targeted drugs.ConclusionThe sigmoid colon cancer organoid with spinal metastases was a favorable preclinical model to explore the clinicopathologic characteristics of colon cancer patients with spinal metastases.https://www.frontiersin.org/articles/10.3389/fcell.2024.1510264/fullsigmoid colon cancerspinal metastasesgenomic featuresorganoid culturedrug screening |
| spellingShingle | Jingyao Chen Jingyao Chen Shumin Cheng Liang Gu Liang Gu Zhangsen Huang Zhangsen Huang Changhua Zhang Changhua Zhang Chunhui Sun Chunhui Sun Chunhui Sun Songyao Chen Songyao Chen Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis Frontiers in Cell and Developmental Biology sigmoid colon cancer spinal metastases genomic features organoid culture drug screening |
| title | Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis |
| title_full | Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis |
| title_fullStr | Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis |
| title_full_unstemmed | Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis |
| title_short | Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis |
| title_sort | establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis |
| topic | sigmoid colon cancer spinal metastases genomic features organoid culture drug screening |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1510264/full |
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