Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization
Osteomyelitis has gradually become a catastrophic complication in orthopedic surgery due to the formation of bacterial biofilms on the implant surface and surrounding tissue. The therapeutic challenges of antibiotic resistance and poor postoperative osseointegration provide inspiration for the devel...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642500002X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841533226838917120 |
|---|---|
| author | Junwei Su Yifan Wu Zheng Wang Dong Zhang Xianquan Yang Yong Zhao Aixi Yu |
| author_facet | Junwei Su Yifan Wu Zheng Wang Dong Zhang Xianquan Yang Yong Zhao Aixi Yu |
| author_sort | Junwei Su |
| collection | DOAJ |
| description | Osteomyelitis has gradually become a catastrophic complication in orthopedic surgery due to the formation of bacterial biofilms on the implant surface and surrounding tissue. The therapeutic challenges of antibiotic resistance and poor postoperative osseointegration provide inspiration for the development of bioactive implants. We have strategically designed bioceramic scaffolds modified with Lactobacillus reuteri (LR) and bacteriophages (phages) to achieve both antibacterial and osteogenic effects. Leveraging the tendency of bacteria to adhere to the surface of implants, bioceramics have been modified with LR biofilm to promote bone repair. The LR biofilm, sterilized by pasteurization, prevents sepsis caused by live bacteria and is biocompatible with phages. Phages, being natural enemies of bacteria, not only effectively kill bacteria and inhibit biofilm formation but also readily adsorb onto the surface of bioceramics. Hence, this scaffold, loaded with a phage cocktail, lysates specific bacterial populations, namely Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). More importantly, the inactivated LR biofilm stimulates macrophages RAW264.7 to polarize towards an anti-inflammatory M2 phenotype, creating an immune microenvironment favorable for inducing osteogenic differentiation of rat mesenchymal stem cells in vitro. In a rat model of infectious cranial defects, the scaffold not only effectively eliminated S. aureus and alleviated associated inflammation but also mediated macrophage-mediated immunoregulation, thus resulting in effective osteogenesis. Collectively, these multifunctional modified scaffolds offer an integrated approach to both bacterium elimination and bone repair, presenting a new strategy for bioactive implants in the clinical management of osteomyelitis. |
| format | Article |
| id | doaj-art-41a58d4a748944ca92da6ef981edfa18 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-41a58d4a748944ca92da6ef981edfa182025-01-17T04:52:15ZengElsevierMaterials Today Bio2590-00642025-02-0130101444Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarizationJunwei Su0Yifan Wu1Zheng Wang2Dong Zhang3Xianquan Yang4Yong Zhao5Aixi Yu6Department of Orthopedic Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, ChinaDepartment of Orthopedic Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, ChinaDepartment of Orthopedic Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, ChinaDepartment of Orthopedic Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, ChinaDepartment of Orthopaedics, Gucheng County People's Hospital, Xiangyang, 441799, Hubei, ChinaDepartment of Orthopedic Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, China; Corresponding author.Department of Orthopedic Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, China; Corresponding author.Osteomyelitis has gradually become a catastrophic complication in orthopedic surgery due to the formation of bacterial biofilms on the implant surface and surrounding tissue. The therapeutic challenges of antibiotic resistance and poor postoperative osseointegration provide inspiration for the development of bioactive implants. We have strategically designed bioceramic scaffolds modified with Lactobacillus reuteri (LR) and bacteriophages (phages) to achieve both antibacterial and osteogenic effects. Leveraging the tendency of bacteria to adhere to the surface of implants, bioceramics have been modified with LR biofilm to promote bone repair. The LR biofilm, sterilized by pasteurization, prevents sepsis caused by live bacteria and is biocompatible with phages. Phages, being natural enemies of bacteria, not only effectively kill bacteria and inhibit biofilm formation but also readily adsorb onto the surface of bioceramics. Hence, this scaffold, loaded with a phage cocktail, lysates specific bacterial populations, namely Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). More importantly, the inactivated LR biofilm stimulates macrophages RAW264.7 to polarize towards an anti-inflammatory M2 phenotype, creating an immune microenvironment favorable for inducing osteogenic differentiation of rat mesenchymal stem cells in vitro. In a rat model of infectious cranial defects, the scaffold not only effectively eliminated S. aureus and alleviated associated inflammation but also mediated macrophage-mediated immunoregulation, thus resulting in effective osteogenesis. Collectively, these multifunctional modified scaffolds offer an integrated approach to both bacterium elimination and bone repair, presenting a new strategy for bioactive implants in the clinical management of osteomyelitis.http://www.sciencedirect.com/science/article/pii/S259000642500002XOsteomyelitisAntibacterialImmunomodulationOsteogenesisPhage therapyProbiotic biofilm |
| spellingShingle | Junwei Su Yifan Wu Zheng Wang Dong Zhang Xianquan Yang Yong Zhao Aixi Yu Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization Materials Today Bio Osteomyelitis Antibacterial Immunomodulation Osteogenesis Phage therapy Probiotic biofilm |
| title | Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization |
| title_full | Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization |
| title_fullStr | Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization |
| title_full_unstemmed | Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization |
| title_short | Probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization |
| title_sort | probiotic biofilm modified scaffolds for facilitating osteomyelitis treatment through sustained release of bacteriophage and regulated macrophage polarization |
| topic | Osteomyelitis Antibacterial Immunomodulation Osteogenesis Phage therapy Probiotic biofilm |
| url | http://www.sciencedirect.com/science/article/pii/S259000642500002X |
| work_keys_str_mv | AT junweisu probioticbiofilmmodifiedscaffoldsforfacilitatingosteomyelitistreatmentthroughsustainedreleaseofbacteriophageandregulatedmacrophagepolarization AT yifanwu probioticbiofilmmodifiedscaffoldsforfacilitatingosteomyelitistreatmentthroughsustainedreleaseofbacteriophageandregulatedmacrophagepolarization AT zhengwang probioticbiofilmmodifiedscaffoldsforfacilitatingosteomyelitistreatmentthroughsustainedreleaseofbacteriophageandregulatedmacrophagepolarization AT dongzhang probioticbiofilmmodifiedscaffoldsforfacilitatingosteomyelitistreatmentthroughsustainedreleaseofbacteriophageandregulatedmacrophagepolarization AT xianquanyang probioticbiofilmmodifiedscaffoldsforfacilitatingosteomyelitistreatmentthroughsustainedreleaseofbacteriophageandregulatedmacrophagepolarization AT yongzhao probioticbiofilmmodifiedscaffoldsforfacilitatingosteomyelitistreatmentthroughsustainedreleaseofbacteriophageandregulatedmacrophagepolarization AT aixiyu probioticbiofilmmodifiedscaffoldsforfacilitatingosteomyelitistreatmentthroughsustainedreleaseofbacteriophageandregulatedmacrophagepolarization |