High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes

High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes

<b>Background/Objectives:</b> Carbapenem-resistant <i>Enterobacterales</i> (CRE) are a global health threat due to their high morbidity and mortality rates and limited treatment options. This study examines the plasmid-mediated transmission of virulence and antibiotic resista...

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Main Authors: Valeria V. Shapovalova, Polina S. Chulkova, Vladimir A. Ageevets, Varvara Nurmukanova, Irina V. Verentsova, Asya A. Girina, Irina N. Protasova, Victoria S. Bezbido, Victor I. Sergevnin, Irina V. Feldblum, Larisa G. Kudryavtseva, Sergey N. Sharafan, Vladislav V. Semerikov, Marina L. Babushkina, Inna R. Valiullina, Nikita S. Chumarev, Guzel S. Isaeva, Natalya A. Belyanina, Irina U. Shirokova, Tatiana M. Mrugova, Elena I. Belkova, Svetlana D. Artemuk, Aleksandra A. Meltser, Marina V. Smirnova, Tatyana N. Akkonen, Nataliya A. Golovshchikova, Oleg V. Goloshchapov, Alexey B. Chukhlovin, Lubov N. Popenko, Elena Y. Zenevich, Aleksandr A. Vlasov, Galina V. Mitroshina, Marina S. Bordacheva, Irina V. Ageevets, Ofeliia S. Sulian, Alisa A. Avdeeva, Vladimir V. Gostev, Irina A. Tsvetkova, Maria A. Yakunina, Ekaterina U. Vasileva, Alina D. Matsvay, Dmitry I. Danilov, Yulia A. Savochkina, German A. Shipulin, Sergey V. Sidorenko
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Antibiotics
Subjects:
<i>Enterobacterales</i>
carbapenem resistance
virulence
plasmids
Online Access:https://www.mdpi.com/2079-6382/13/12/1224
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Summary:<b>Background/Objectives:</b> Carbapenem-resistant <i>Enterobacterales</i> (CRE) are a global health threat due to their high morbidity and mortality rates and limited treatment options. This study examines the plasmid-mediated transmission of virulence and antibiotic resistance determinants in carbapenem-resistant <i>Klebsiella pneumoniae</i> (<i>Kpn</i>) and <i>Escherichia coli</i> (<i>E. coli</i>) isolated from Russian hospitals. <b>Methods</b>: We performed short- and long-read whole-genome sequencing of 53 clinical isolates (48 <i>Kpn</i> and 5 <i>E. coli</i>) attributed to 15 genetic lineages and collected from 21 hospitals across nine Russian cities between 2016 and 2022. <b>Results</b>: The plasmid analysis identified 18 clusters that showed high concordance with replicon typing, with all clusters having a major replicon type. The majority of plasmids in the IncHI1B(pNDM-MAR)/IncFIB(pNDM-Mar)-like cluster (79.16%) carried both antibiotic resistance genes (e.g., <i>bla</i><sub>NDM-1</sub> and <i>bla</i><sub>OXA-48</sub>) and virulence factors (VFs) such as siderophore genes. We hypothesized that hybrid plasmids could play a critical role in the dissemination of antibiotic resistance genes and VFs. Comparative analyses with global plasmid databases revealed high-risk lineages of hybrid plasmids that are predominantly spread throughout Russia at present. <b>Conclusions:</b> Our findings underscore the importance of monitoring plasmid backbones for clinical management, surveillance, and infection control activities.
ISSN:2079-6382

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