In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha protein
Abstract Objective This research investigates the potential anti-tumour effects of bromelain, an aqueous extract from pineapple stems and fruits, on nasopharyngeal cancer (NPC). While bromelain is known for its medicinal properties in various cancers, its impact on NPC remains unexplored. Results Us...
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BMC
2024-11-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-024-06995-2 |
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| author | Alyaa Syafiqah Shamsuri Edmund Ui -Hang Sim |
| author_facet | Alyaa Syafiqah Shamsuri Edmund Ui -Hang Sim |
| author_sort | Alyaa Syafiqah Shamsuri |
| collection | DOAJ |
| description | Abstract Objective This research investigates the potential anti-tumour effects of bromelain, an aqueous extract from pineapple stems and fruits, on nasopharyngeal cancer (NPC). While bromelain is known for its medicinal properties in various cancers, its impact on NPC remains unexplored. Results Using in silico methods, we studied the predicted interactions between bromelain and key proteins involved in NPC oncogenesis, specifically β-catenin, PIK3CA, mTOR, EGFR, and BCL2. Molecular docking strategies were performed using a myriad of computational tools. A 3D model of bromelain was constructed using SWISS-MODEL, followed by molecular docking simulations performed with ClusPro. The binding affinities of the docked complexes were evaluated using HawkDock, and the interactions were analysed with LigPlot+. The docking scores indicated potential spontaneous interactions, with binding affinities based on being − 103.89 kcal/mol (PIK3CA), -73.16 kcal/mol (EGFR), -71.18 kcal/mol (mTOR), -65.22 kcal/mol (β-catenin), and − 57.48 kcal/mol (BCL2). LigPlot + analysis revealed the presence of hydrogen bonds, hydrophobic interactions, and salt bridges, indicating stable predicted interactions. Conclusion Our findings suggest that bromelain can target key proteins involved in NPC oncogenesis, with the strongest affinity towards PIK3CA. This suggests a hypothetical insight into bromelain’s anticancer effects on NPC through the modulation of the PI3K/Akt signaling pathway. |
| format | Article |
| id | doaj-art-4160c12ed09c495dab6247b8c55974db |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-4160c12ed09c495dab6247b8c55974db2024-12-01T12:08:17ZengBMCBMC Research Notes1756-05002024-11-011711810.1186/s13104-024-06995-2In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha proteinAlyaa Syafiqah Shamsuri0Edmund Ui -Hang Sim1Faculty of Resource Science and Technology, Universiti Malaysia SarawakFaculty of Resource Science and Technology, Universiti Malaysia SarawakAbstract Objective This research investigates the potential anti-tumour effects of bromelain, an aqueous extract from pineapple stems and fruits, on nasopharyngeal cancer (NPC). While bromelain is known for its medicinal properties in various cancers, its impact on NPC remains unexplored. Results Using in silico methods, we studied the predicted interactions between bromelain and key proteins involved in NPC oncogenesis, specifically β-catenin, PIK3CA, mTOR, EGFR, and BCL2. Molecular docking strategies were performed using a myriad of computational tools. A 3D model of bromelain was constructed using SWISS-MODEL, followed by molecular docking simulations performed with ClusPro. The binding affinities of the docked complexes were evaluated using HawkDock, and the interactions were analysed with LigPlot+. The docking scores indicated potential spontaneous interactions, with binding affinities based on being − 103.89 kcal/mol (PIK3CA), -73.16 kcal/mol (EGFR), -71.18 kcal/mol (mTOR), -65.22 kcal/mol (β-catenin), and − 57.48 kcal/mol (BCL2). LigPlot + analysis revealed the presence of hydrogen bonds, hydrophobic interactions, and salt bridges, indicating stable predicted interactions. Conclusion Our findings suggest that bromelain can target key proteins involved in NPC oncogenesis, with the strongest affinity towards PIK3CA. This suggests a hypothetical insight into bromelain’s anticancer effects on NPC through the modulation of the PI3K/Akt signaling pathway.https://doi.org/10.1186/s13104-024-06995-2Nasopharyngeal carcinomaMolecular docking simulationProtein-protein interactionsBromelainPIK3CA |
| spellingShingle | Alyaa Syafiqah Shamsuri Edmund Ui -Hang Sim In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha protein BMC Research Notes Nasopharyngeal carcinoma Molecular docking simulation Protein-protein interactions Bromelain PIK3CA |
| title | In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha protein |
| title_full | In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha protein |
| title_fullStr | In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha protein |
| title_full_unstemmed | In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha protein |
| title_short | In silico prediction of the action of bromelain on PI3K/Akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha protein |
| title_sort | in silico prediction of the action of bromelain on pi3k akt signalling pathway to arrest nasopharyngeal cancer oncogenesis by targeting phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha protein |
| topic | Nasopharyngeal carcinoma Molecular docking simulation Protein-protein interactions Bromelain PIK3CA |
| url | https://doi.org/10.1186/s13104-024-06995-2 |
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