Shining a Light on Spectrophotometry in Bacteriology
Spectrophotometry is widely used in biological sciences. In bacteriology, spectrophotometric monitoring of cell numbers during cultivation provides a rapid assessment of growth. Unfortunately, familiarity with this technique has led scientists to become complacent in its usage. Here, we review some...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/12/1164 |
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| _version_ | 1846106196615888896 |
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| author | Veronica Montesinos-Cruz Greg A. Somerville |
| author_facet | Veronica Montesinos-Cruz Greg A. Somerville |
| author_sort | Veronica Montesinos-Cruz |
| collection | DOAJ |
| description | Spectrophotometry is widely used in biological sciences. In bacteriology, spectrophotometric monitoring of cell numbers during cultivation provides a rapid assessment of growth. Unfortunately, familiarity with this technique has led scientists to become complacent in its usage. Here, we review some guiding principles of spectrophotometry and practical considerations that may influence the outcome of experiments. This perspective is intended to assist both new and seasoned scientists in presenting robust and reproducible growth data based on spectrophotometric readings. |
| format | Article |
| id | doaj-art-4115e21c26f544ce87ef3f3b5c0e192d |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-4115e21c26f544ce87ef3f3b5c0e192d2024-12-27T14:06:13ZengMDPI AGAntibiotics2079-63822024-12-011312116410.3390/antibiotics13121164Shining a Light on Spectrophotometry in BacteriologyVeronica Montesinos-Cruz0Greg A. Somerville1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905, USASchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905, USASpectrophotometry is widely used in biological sciences. In bacteriology, spectrophotometric monitoring of cell numbers during cultivation provides a rapid assessment of growth. Unfortunately, familiarity with this technique has led scientists to become complacent in its usage. Here, we review some guiding principles of spectrophotometry and practical considerations that may influence the outcome of experiments. This perspective is intended to assist both new and seasoned scientists in presenting robust and reproducible growth data based on spectrophotometric readings.https://www.mdpi.com/2079-6382/13/12/1164spectrophotometrylight scatteringbacterial quantitationantibiotic resistancepersistence |
| spellingShingle | Veronica Montesinos-Cruz Greg A. Somerville Shining a Light on Spectrophotometry in Bacteriology Antibiotics spectrophotometry light scattering bacterial quantitation antibiotic resistance persistence |
| title | Shining a Light on Spectrophotometry in Bacteriology |
| title_full | Shining a Light on Spectrophotometry in Bacteriology |
| title_fullStr | Shining a Light on Spectrophotometry in Bacteriology |
| title_full_unstemmed | Shining a Light on Spectrophotometry in Bacteriology |
| title_short | Shining a Light on Spectrophotometry in Bacteriology |
| title_sort | shining a light on spectrophotometry in bacteriology |
| topic | spectrophotometry light scattering bacterial quantitation antibiotic resistance persistence |
| url | https://www.mdpi.com/2079-6382/13/12/1164 |
| work_keys_str_mv | AT veronicamontesinoscruz shiningalightonspectrophotometryinbacteriology AT gregasomerville shiningalightonspectrophotometryinbacteriology |