Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch
Abstract Design and synthesis of basic functional circuits are the fundamental tasks of synthetic biologists. Before it is possible to engineer higher‐order genetic networks that can perform complex functions, a toolkit of basic devices must be developed. Among those devices, sequential logic circui...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2010-03-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2010.2 |
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| _version_ | 1849225756665708544 |
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| author | Chunbo Lou Xili Liu Ming Ni Yiqi Huang Qiushi Huang Longwen Huang Lingli Jiang Dan Lu Mingcong Wang Chang Liu Daizhuo Chen Chongyi Chen Xiaoyue Chen Le Yang Haisu Ma Jianguo Chen Qi Ouyang |
| author_facet | Chunbo Lou Xili Liu Ming Ni Yiqi Huang Qiushi Huang Longwen Huang Lingli Jiang Dan Lu Mingcong Wang Chang Liu Daizhuo Chen Chongyi Chen Xiaoyue Chen Le Yang Haisu Ma Jianguo Chen Qi Ouyang |
| author_sort | Chunbo Lou |
| collection | DOAJ |
| description | Abstract Design and synthesis of basic functional circuits are the fundamental tasks of synthetic biologists. Before it is possible to engineer higher‐order genetic networks that can perform complex functions, a toolkit of basic devices must be developed. Among those devices, sequential logic circuits are expected to be the foundation of the genetic information‐processing systems. In this study, we report the design and construction of a genetic sequential logic circuit in Escherichia coli. It can generate different outputs in response to the same input signal on the basis of its internal state, and ‘memorize’ the output. The circuit is composed of two parts: (1) a bistable switch memory module and (2) a double‐repressed promoter NOR gate module. The two modules were individually rationally designed, and they were coupled together by fine‐tuning the interconnecting parts through directed evolution. After fine‐tuning, the circuit could be repeatedly, alternatively triggered by the same input signal; it functions as a push‐on push‐off switch. |
| format | Article |
| id | doaj-art-41000a5c0cf9485b81a7e52000e28d6c |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2010-03-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-41000a5c0cf9485b81a7e52000e28d6c2025-08-24T12:00:55ZengSpringer NatureMolecular Systems Biology1744-42922010-03-016111110.1038/msb.2010.2Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switchChunbo Lou0Xili Liu1Ming Ni2Yiqi Huang3Qiushi Huang4Longwen Huang5Lingli Jiang6Dan Lu7Mingcong Wang8Chang Liu9Daizhuo Chen10Chongyi Chen11Xiaoyue Chen12Le Yang13Haisu Ma14Jianguo Chen15Qi Ouyang162007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking UniversityCenter for Theoretical Biology, Peking UniversityCenter for Theoretical Biology, Peking UniversityCenter for Theoretical Biology, Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking University2007 Peking University Team for The International Genetic Engineering Machine Competition (iGEM), Peking UniversityCollege of Life Science, Peking UniversityCenter for Theoretical Biology, Peking UniversityAbstract Design and synthesis of basic functional circuits are the fundamental tasks of synthetic biologists. Before it is possible to engineer higher‐order genetic networks that can perform complex functions, a toolkit of basic devices must be developed. Among those devices, sequential logic circuits are expected to be the foundation of the genetic information‐processing systems. In this study, we report the design and construction of a genetic sequential logic circuit in Escherichia coli. It can generate different outputs in response to the same input signal on the basis of its internal state, and ‘memorize’ the output. The circuit is composed of two parts: (1) a bistable switch memory module and (2) a double‐repressed promoter NOR gate module. The two modules were individually rationally designed, and they were coupled together by fine‐tuning the interconnecting parts through directed evolution. After fine‐tuning, the circuit could be repeatedly, alternatively triggered by the same input signal; it functions as a push‐on push‐off switch.https://doi.org/10.1038/msb.2010.2bistable switchcoupling modulesgenetic sequential logic circuitNOR gatepush‐on push‐off switch |
| spellingShingle | Chunbo Lou Xili Liu Ming Ni Yiqi Huang Qiushi Huang Longwen Huang Lingli Jiang Dan Lu Mingcong Wang Chang Liu Daizhuo Chen Chongyi Chen Xiaoyue Chen Le Yang Haisu Ma Jianguo Chen Qi Ouyang Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch Molecular Systems Biology bistable switch coupling modules genetic sequential logic circuit NOR gate push‐on push‐off switch |
| title | Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch |
| title_full | Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch |
| title_fullStr | Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch |
| title_full_unstemmed | Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch |
| title_short | Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch |
| title_sort | synthesizing a novel genetic sequential logic circuit a push on push off switch |
| topic | bistable switch coupling modules genetic sequential logic circuit NOR gate push‐on push‐off switch |
| url | https://doi.org/10.1038/msb.2010.2 |
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