Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits
Objective Dermal papilla cells (DPCs) play a pivotal role in hair follicle development and can modulate melanogenesis in melanocytes (MCs) through their microenvironment. Our previous studies have demonstrated that the levels of exosomal miR-222-3p derived from DPCs of white Rex rabbits are signific...
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| Format: | Article |
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Asian-Australasian Association of Animal Production Societies
2025-02-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0182.pdf |
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| author | Yang Chen Tingting Lu Yingying Dai Yu Xue Bohao Zhao Xinsheng Wu |
| author_facet | Yang Chen Tingting Lu Yingying Dai Yu Xue Bohao Zhao Xinsheng Wu |
| author_sort | Yang Chen |
| collection | DOAJ |
| description | Objective Dermal papilla cells (DPCs) play a pivotal role in hair follicle development and can modulate melanogenesis in melanocytes (MCs) through their microenvironment. Our previous studies have demonstrated that the levels of exosomal miR-222-3p derived from DPCs of white Rex rabbits are significantly higher than those of black Rex rabbits. However, the specific role and underlying molecular mechanisms of exosomal miR-222-3p in melanogenesis remain elusive. Methods DPCs and MCs were isolated from hair follicles of Rex rabbits and identified using western blotting (WB) and immunofluorescent staining. Exosomes derived from DPCs (DPCs-exos) were characterized using nanoparticle tracking analysis, transmission electron microscopy, and WB. To investigate cell-cell crosstalk mediated by exosomes, MCs were co-cultured with CM-Dil-labeled DPCs-exos. The expression of miR-222-3p in skin tissue and exosomes was quantitatively assessed using quantitative real-time polymerase chain reaction. The transmission of DPCs-secreted exosomal miR-222-3p to MCs was demonstrated using Cy3-labeled miR-222-3p in conjunction with transwell assays. The impact of miR-222-3p on melanin synthesis was evaluated using the NaOH method, cell counting kit-8, and annexin V-fluorescein isothiocyanate/propidium iodide assays. Sex determining region Y-box 10 (SOX10), a potential target gene regulated by miR-222-3p, was validated using a dual-luciferase reporter assay, site-specific mutation, and WB. Results Increased levels of miR-222-3p were observed in the skin and DPCs-exos of white Rex rabbits compared to those of black Rex rabbits. Effective internalization of CM-Dil-labeled DPCs-exos by MCs was observed. Furthermore, exosomal miR-222-3p derived from DPCs was transferred to MCs. Functionally, miR-222-3p significantly inhibited MCs proliferation, induced apoptosis and inhibited melanin synthesis. SOX10 was confirmed as a direct target of miR-222-3p in this regulatory cascade. Conclusion The findings demonstrate that exosomal miR-222-3p, derived from DPCs, suppresses melanogenesis in MCs by targeting SOX10, thus unveiling a novel mechanism of exosome involvement in melanogenesis. |
| format | Article |
| id | doaj-art-40edd6821dc4405986791ffe30318aef |
| institution | Kabale University |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
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| series | Animal Bioscience |
| spelling | doaj-art-40edd6821dc4405986791ffe30318aef2025-01-03T04:14:22ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-02-0138223624610.5713/ab.24.018225336Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbitsYang Chen0Tingting Lu1Yingying Dai2Yu Xue3Bohao Zhao4Xinsheng Wu5 College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaObjective Dermal papilla cells (DPCs) play a pivotal role in hair follicle development and can modulate melanogenesis in melanocytes (MCs) through their microenvironment. Our previous studies have demonstrated that the levels of exosomal miR-222-3p derived from DPCs of white Rex rabbits are significantly higher than those of black Rex rabbits. However, the specific role and underlying molecular mechanisms of exosomal miR-222-3p in melanogenesis remain elusive. Methods DPCs and MCs were isolated from hair follicles of Rex rabbits and identified using western blotting (WB) and immunofluorescent staining. Exosomes derived from DPCs (DPCs-exos) were characterized using nanoparticle tracking analysis, transmission electron microscopy, and WB. To investigate cell-cell crosstalk mediated by exosomes, MCs were co-cultured with CM-Dil-labeled DPCs-exos. The expression of miR-222-3p in skin tissue and exosomes was quantitatively assessed using quantitative real-time polymerase chain reaction. The transmission of DPCs-secreted exosomal miR-222-3p to MCs was demonstrated using Cy3-labeled miR-222-3p in conjunction with transwell assays. The impact of miR-222-3p on melanin synthesis was evaluated using the NaOH method, cell counting kit-8, and annexin V-fluorescein isothiocyanate/propidium iodide assays. Sex determining region Y-box 10 (SOX10), a potential target gene regulated by miR-222-3p, was validated using a dual-luciferase reporter assay, site-specific mutation, and WB. Results Increased levels of miR-222-3p were observed in the skin and DPCs-exos of white Rex rabbits compared to those of black Rex rabbits. Effective internalization of CM-Dil-labeled DPCs-exos by MCs was observed. Furthermore, exosomal miR-222-3p derived from DPCs was transferred to MCs. Functionally, miR-222-3p significantly inhibited MCs proliferation, induced apoptosis and inhibited melanin synthesis. SOX10 was confirmed as a direct target of miR-222-3p in this regulatory cascade. Conclusion The findings demonstrate that exosomal miR-222-3p, derived from DPCs, suppresses melanogenesis in MCs by targeting SOX10, thus unveiling a novel mechanism of exosome involvement in melanogenesis.http://www.animbiosci.org/upload/pdf/ab-24-0182.pdfdermal papilla cellsexosomemelanocytesmelanogenesismir-222-3p |
| spellingShingle | Yang Chen Tingting Lu Yingying Dai Yu Xue Bohao Zhao Xinsheng Wu Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits Animal Bioscience dermal papilla cells exosome melanocytes melanogenesis mir-222-3p |
| title | Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits |
| title_full | Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits |
| title_fullStr | Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits |
| title_full_unstemmed | Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits |
| title_short | Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits |
| title_sort | exosomal mir 222 3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting sox10 in rabbits |
| topic | dermal papilla cells exosome melanocytes melanogenesis mir-222-3p |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0182.pdf |
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