Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice
Glume-opening of thermosensitive genic male sterile (TGMS) rice (<i>Oryza sativa</i> L.) lines after anthesis is a serious problem that significantly reduces the yield and quality of hybrid seeds. However, the molecular mechanisms regulating the opening and closing of rice glumes remain...
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2024-12-01
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| author | Xinhui Zhao Mengyi Wei Qianying Tang Lei Tang Jun Fu Kai Wang Yanbiao Zhou Yuanzhu Yang |
| author_facet | Xinhui Zhao Mengyi Wei Qianying Tang Lei Tang Jun Fu Kai Wang Yanbiao Zhou Yuanzhu Yang |
| author_sort | Xinhui Zhao |
| collection | DOAJ |
| description | Glume-opening of thermosensitive genic male sterile (TGMS) rice (<i>Oryza sativa</i> L.) lines after anthesis is a serious problem that significantly reduces the yield and quality of hybrid seeds. However, the molecular mechanisms regulating the opening and closing of rice glumes remain largely unclear. In this study, we report the isolation and functional characterization of a glum-opening mutant after anthesis, named <i>gom1</i>. <i>gom1</i> exhibits dysfunctional lodicules that lead to open glumes following anthesis. Map-based cloning and subsequent complementation tests confirmed that GOM1 encodes a receptor-like kinase (RLK). <i>GOM1</i> was expressed in nearly all floral tissues, with the highest expression in the lodicule. Loss-of-function of <i>GOM1</i> resulted in a decrease in the expression of genes related to JA biosynthesis, JA signaling, and sugar transport. Compared with LK638S, the JA content in the <i>gom1</i> mutant was significantly reduced, while the soluble sugar, sucrose, glucose, and fructose contents were significantly increased in lodicules after anthesis. Together, we speculated that GOM1 regulates carbohydrate transport in lodicules during anthesis through JA and JA signaling, maintaining a higher osmolality in lodicules after anthesis, which leads to glum-opening. |
| format | Article |
| id | doaj-art-40b51fd6602444e8b0ce87b44fd7d160 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-40b51fd6602444e8b0ce87b44fd7d1602025-01-10T13:19:27ZengMDPI AGPlants2223-77472024-12-01141510.3390/plants14010005Receptor-like Kinase GOM1 Regulates Glume-Opening in RiceXinhui Zhao0Mengyi Wei1Qianying Tang2Lei Tang3Jun Fu4Kai Wang5Yanbiao Zhou6Yuanzhu Yang7College of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Life Sciences, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd., Changsha 410001, ChinaCollege of Life Sciences, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd., Changsha 410001, ChinaKey Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd., Changsha 410001, ChinaKey Laboratory of Southern Rice Innovation & Improvement, Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd., Changsha 410001, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaGlume-opening of thermosensitive genic male sterile (TGMS) rice (<i>Oryza sativa</i> L.) lines after anthesis is a serious problem that significantly reduces the yield and quality of hybrid seeds. However, the molecular mechanisms regulating the opening and closing of rice glumes remain largely unclear. In this study, we report the isolation and functional characterization of a glum-opening mutant after anthesis, named <i>gom1</i>. <i>gom1</i> exhibits dysfunctional lodicules that lead to open glumes following anthesis. Map-based cloning and subsequent complementation tests confirmed that GOM1 encodes a receptor-like kinase (RLK). <i>GOM1</i> was expressed in nearly all floral tissues, with the highest expression in the lodicule. Loss-of-function of <i>GOM1</i> resulted in a decrease in the expression of genes related to JA biosynthesis, JA signaling, and sugar transport. Compared with LK638S, the JA content in the <i>gom1</i> mutant was significantly reduced, while the soluble sugar, sucrose, glucose, and fructose contents were significantly increased in lodicules after anthesis. Together, we speculated that GOM1 regulates carbohydrate transport in lodicules during anthesis through JA and JA signaling, maintaining a higher osmolality in lodicules after anthesis, which leads to glum-opening.https://www.mdpi.com/2223-7747/14/1/5glum-openinglodiculeanthesisjasmonic acidcarbohydraterice |
| spellingShingle | Xinhui Zhao Mengyi Wei Qianying Tang Lei Tang Jun Fu Kai Wang Yanbiao Zhou Yuanzhu Yang Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice Plants glum-opening lodicule anthesis jasmonic acid carbohydrate rice |
| title | Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice |
| title_full | Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice |
| title_fullStr | Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice |
| title_full_unstemmed | Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice |
| title_short | Receptor-like Kinase GOM1 Regulates Glume-Opening in Rice |
| title_sort | receptor like kinase gom1 regulates glume opening in rice |
| topic | glum-opening lodicule anthesis jasmonic acid carbohydrate rice |
| url | https://www.mdpi.com/2223-7747/14/1/5 |
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