Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells
Thymoquinone(Tq; 2-isopropyl-5-methyl-1,4 benzoquinone) is the main ingredient present inthe volatile oil of Nigella sativa(black seed). Recently, it has been reported that, Tq was inhibited cellproliferation of many cancer cell lines including ovarian, lung, breast, osteosarcoma,pancreatic, fibrosa...
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| Format: | Article |
| Language: | English |
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Sakarya University
2020-02-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/967514 |
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| Summary: | Thymoquinone(Tq; 2-isopropyl-5-methyl-1,4 benzoquinone) is the main ingredient present inthe volatile oil of Nigella sativa(black seed). Recently, it has been reported that, Tq was inhibited cellproliferation of many cancer cell lines including ovarian, lung, breast, osteosarcoma,pancreatic, fibrosarcoma, lung cancer, squamous cell carcinoma and myeloblasticleukemias. In this study, the anticancer effectof Tq was investigated in human Colon Cancer Cells (HT-29) with MTT assay.Positive control was achieved with 5-fluorouracil (5-FU). Also, the invasioncapability of HT-29 cellls was determined in presence of Tq and 5-FU withscratch analyses. In addition, the autophagic and apoptotic effect of Tq wereinvestigated by qRT-PCR method to find out the pathway of anti-proliferativeeffect in Tq-treated HT-29 cells. As a result, Tq was inhibited the cellproliferation of HT-29 cells in time dependent manner, with 118 µM for 24 h and 84 µM for 48 h, respectively. Aconcentration of 150 µM of Tq was able to significantly reduced theinvasiveness of HT-29 cells about ̴8fold compared with not treated cells at 6h. ATG-12, ATG-7 and LC3-II weresignificantly downregulated in presence of Tq. Conversaly, the expression of all of theautophagy genes were downregulated in presence of 5-FU. Moreover, pro-apoptoticgene Bax was significiantly upregulated nearly 15 fold whereas the expressionsof Bcl-2 and Bcl-XL (pro-survival genes) were decreased in presence of Tq. Onthe other hand, in 5-FU treated HT-29 cells, the expression of Bax, Bcl-2 and Bcl-XL genes andautophagy related genes were significantly downregulated. |
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| ISSN: | 2147-835X |