Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation
ABSTRACT: The study evaluated strategies for induction of ovulation at the end of timed AI (TAI) protocols initiated after a novel presynchronization strategy. A total of 909 lactating dairy cows from 6 dairy herds initiated a presynchronization protocol on d −15 with an intravaginal progesterone (P...
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Elsevier
2025-01-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224012335 |
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| author | Carlos E.C. Consentini Leonardo F. Melo Jessica C. Lemos Motta Lucas Oliveira e Silva Natália Picoli Folchini Rodrigo L.O.R. Alves Guilherme Madureira Tiago Oliveira Carneiro Tattiany Abadia Luis Moroz Robson V.S. Fortes Sergio Soriano Bruno Gonzales Milo C. Wiltbank Roberto Sartori |
| author_facet | Carlos E.C. Consentini Leonardo F. Melo Jessica C. Lemos Motta Lucas Oliveira e Silva Natália Picoli Folchini Rodrigo L.O.R. Alves Guilherme Madureira Tiago Oliveira Carneiro Tattiany Abadia Luis Moroz Robson V.S. Fortes Sergio Soriano Bruno Gonzales Milo C. Wiltbank Roberto Sartori |
| author_sort | Carlos E.C. Consentini |
| collection | DOAJ |
| description | ABSTRACT: The study evaluated strategies for induction of ovulation at the end of timed AI (TAI) protocols initiated after a novel presynchronization strategy. A total of 909 lactating dairy cows from 6 dairy herds initiated a presynchronization protocol on d −15 with an intravaginal progesterone (P4) implant and 7 d later (d −8) were treated with 1.0 mg of estradiol cypionate (EC) and 0.530 mg of cloprostenol sodium (a synthetic analog of prostaglandin F2α; PGF), simultaneously with the P4 implant removal. On d 0, the protocol for synchronization of ovulation was initiated with 16.8 µg of buserelin acetate (a synthetic analog of GnRH) and insertion of a 2.0 g P4 implant, followed by a treatment with 0.530 mg of PGF on d 6, and a second PGF on d 7, concomitant with the P4 implant withdrawal. In Group EC, cows received 1.0 mg of EC on d 7 as an ovulation inducer. In G group, cows received 8.4 µg of GnRH 56 h after the first PGF (16 h before TAI). In Group EC/G, cows received both EC and GnRH. The TAI was performed on d 9 (48 h after P4 withdrawal) in all experimental treatments. There were no differences among treatments on pregnancy per AI (P/AI) on d 31 (40.4%; 367/909). There was a tendency for greater pregnancy loss in G group (19.8%) than in EC (12.2%) and EC/G (10.1%) groups. When the 2 groups that received EC were combined, the pregnancy loss was lower than when cows received only GnRH (11.2% [21/188] vs. 19.8% [17/86]; P = 0.05). There was an interaction between treatment and expression of estrus on P/AI, in which estrus positively affected P/AI in EC (53.3% vs. 20.0%) and EC/G (53.1% vs. 36.7%), but not in G group (41.5% vs. 38.7%). Overall, 81% of the cows had a corpus luteum (CL) on d 0 and 91% at PGF treatment of the breeding protocols. Fertility was greater in cows with CL at the time of the first PGF treatment than in cows without CL (45.9% vs. 17.7%). In summary, the strategies to induce final ovulation resulted in similar fertility despite the different physiological responses, such as expression of estrus, but further research is needed to definitively confirm the relationship between lack of EC and increased pregnancy loss. |
| format | Article |
| id | doaj-art-404d9815b0a44b348afa62e620ade6b6 |
| institution | Kabale University |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Journal of Dairy Science |
| spelling | doaj-art-404d9815b0a44b348afa62e620ade6b62024-12-26T08:52:17ZengElsevierJournal of Dairy Science0022-03022025-01-01108111381149Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulationCarlos E.C. Consentini0Leonardo F. Melo1Jessica C. Lemos Motta2Lucas Oliveira e Silva3Natália Picoli Folchini4Rodrigo L.O.R. Alves5Guilherme Madureira6Tiago Oliveira Carneiro7Tattiany Abadia8Luis Moroz9Robson V.S. Fortes10Sergio Soriano11Bruno Gonzales12Milo C. Wiltbank13Roberto Sartori14Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900; Department of Animal and Dairy Sciences, University of Wisconsin–Madison, Madison, WI 53706; GlobalGen Vet Science, Jaboticabal, SP, Brazil, 14887-360School of Veterinary and Animal Sciences, Federal University of Goiás, Goiânia, GO, Brazil, 74690-900Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900Bela Vista Farm, Tapiratiba, SP, Brazil, 13760-000Céu Azul Farm, Silvânia, GO, Brazil, 75180-000Frank'Anna Farm, Carambeí, PR, Brazil, 84145-000Barreiro Farm, Carmo do Rio Claro, MG, Brazil, 37150-000Colorado Farm, Araras, SP, Brazil, 13600-970São Jorge Farm, São Pedro, SP, Brazil, 13520-000Department of Animal and Dairy Sciences, University of Wisconsin–Madison, Madison, WI 53706Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900; Corresponding authorABSTRACT: The study evaluated strategies for induction of ovulation at the end of timed AI (TAI) protocols initiated after a novel presynchronization strategy. A total of 909 lactating dairy cows from 6 dairy herds initiated a presynchronization protocol on d −15 with an intravaginal progesterone (P4) implant and 7 d later (d −8) were treated with 1.0 mg of estradiol cypionate (EC) and 0.530 mg of cloprostenol sodium (a synthetic analog of prostaglandin F2α; PGF), simultaneously with the P4 implant removal. On d 0, the protocol for synchronization of ovulation was initiated with 16.8 µg of buserelin acetate (a synthetic analog of GnRH) and insertion of a 2.0 g P4 implant, followed by a treatment with 0.530 mg of PGF on d 6, and a second PGF on d 7, concomitant with the P4 implant withdrawal. In Group EC, cows received 1.0 mg of EC on d 7 as an ovulation inducer. In G group, cows received 8.4 µg of GnRH 56 h after the first PGF (16 h before TAI). In Group EC/G, cows received both EC and GnRH. The TAI was performed on d 9 (48 h after P4 withdrawal) in all experimental treatments. There were no differences among treatments on pregnancy per AI (P/AI) on d 31 (40.4%; 367/909). There was a tendency for greater pregnancy loss in G group (19.8%) than in EC (12.2%) and EC/G (10.1%) groups. When the 2 groups that received EC were combined, the pregnancy loss was lower than when cows received only GnRH (11.2% [21/188] vs. 19.8% [17/86]; P = 0.05). There was an interaction between treatment and expression of estrus on P/AI, in which estrus positively affected P/AI in EC (53.3% vs. 20.0%) and EC/G (53.1% vs. 36.7%), but not in G group (41.5% vs. 38.7%). Overall, 81% of the cows had a corpus luteum (CL) on d 0 and 91% at PGF treatment of the breeding protocols. Fertility was greater in cows with CL at the time of the first PGF treatment than in cows without CL (45.9% vs. 17.7%). In summary, the strategies to induce final ovulation resulted in similar fertility despite the different physiological responses, such as expression of estrus, but further research is needed to definitively confirm the relationship between lack of EC and increased pregnancy loss.http://www.sciencedirect.com/science/article/pii/S0022030224012335timed AIsynchronization of ovulationexpression of estrusfertilitydairy cow |
| spellingShingle | Carlos E.C. Consentini Leonardo F. Melo Jessica C. Lemos Motta Lucas Oliveira e Silva Natália Picoli Folchini Rodrigo L.O.R. Alves Guilherme Madureira Tiago Oliveira Carneiro Tattiany Abadia Luis Moroz Robson V.S. Fortes Sergio Soriano Bruno Gonzales Milo C. Wiltbank Roberto Sartori Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation Journal of Dairy Science timed AI synchronization of ovulation expression of estrus fertility dairy cow |
| title | Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation |
| title_full | Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation |
| title_fullStr | Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation |
| title_full_unstemmed | Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation |
| title_short | Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation |
| title_sort | reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation |
| topic | timed AI synchronization of ovulation expression of estrus fertility dairy cow |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224012335 |
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