Transcriptomics and Metabolomics Explain the Crisping Mechanisms of Broad Bean-Based Crisping Diets on Nile Tilapia (<i>Orechromis niloticus</i>)

Transcriptomics and Metabolomics Explain the Crisping Mechanisms of Broad Bean-Based Crisping Diets on Nile Tilapia (<i>Orechromis niloticus</i>)

<b>Background/Objectives:</b> To investigate the crisping mechanism of broad bean-based crisping diets on Nile Tilapia. <b>Methods:</b> Four crisping diets were designed to feed 360 fish for 90 days, and multiomics analyses were employed. <b>Results:</b> Our resul...

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Bibliographic Details
Main Authors: Xiaogang He, Haoming Shu, Tian Xu, Minhui Yu, Honglin Li, Yanru Hu, Jiajun Mo, Chunxiang Ai
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Metabolites
Subjects:
broad bean
crisping diet
Nile tilapia
metabolism reprogramming
glucose metabolism
Online Access:https://www.mdpi.com/2218-1989/14/11/616
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Summary:<b>Background/Objectives:</b> To investigate the crisping mechanism of broad bean-based crisping diets on Nile Tilapia. <b>Methods:</b> Four crisping diets were designed to feed 360 fish for 90 days, and multiomics analyses were employed. <b>Results:</b> Our results indicated that the designed crisping diets for Nile tilapia can effectively make tilapia muscle crispy. The ingestion of broad bean-based diets induced metabolic reprogramming dominated by glycolytic metabolism inhibition in fish, and metabolic reprogramming is the initiator of muscle structural remodeling. Among these, glucose is the main DAMP to be recognized by cellular PRRs, activating further immune response and oxidative stress and finally resulting in muscle change. <b>Conclusions:</b> Based on our results of multiomics, <i>pck2</i>, and <i>ldh</i> played main roles in crisping molecular mechanisms in driving the initial metabolic reprogram. Moreover, the addition of the crisping package further activated the ErbB signaling pathway and downstream MAPK signaling pathway to strengthen immune response, promoting muscle fiber development and growth. Our study delved into the effects of crisping formula diet on the liver of Nile tilapia at the molecular level, providing theoretical guidance for the nutritional regulation of crispy Nile tilapia.
ISSN:2218-1989

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