Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo
BackgroundGardnerella is a key pathogen in bacterial vaginosis (BV), but the role of the different Gardnerella species remains unclear. We investigated the role of four Gardnerella species, as well as Fannyhessea vaginae, Lactobacillus crispatus and L. iners in BV.MethodsFrom 331 pregnant women from...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| author | Lisa Himschoot Guy Mulinganya Guy Mulinganya Guy Mulinganya Tess Rogier Ghislain Bisimwa Ghislain Bisimwa Freddy Kampara Freddy Kampara Yvette Kujirakwinja Yvette Kujirakwinja Jules Mongane Jules Mongane Innocent Mubalama Steven Callens Mario Vaneechoutte Piet Cools |
| author_facet | Lisa Himschoot Guy Mulinganya Guy Mulinganya Guy Mulinganya Tess Rogier Ghislain Bisimwa Ghislain Bisimwa Freddy Kampara Freddy Kampara Yvette Kujirakwinja Yvette Kujirakwinja Jules Mongane Jules Mongane Innocent Mubalama Steven Callens Mario Vaneechoutte Piet Cools |
| author_sort | Lisa Himschoot |
| collection | DOAJ |
| description | BackgroundGardnerella is a key pathogen in bacterial vaginosis (BV), but the role of the different Gardnerella species remains unclear. We investigated the role of four Gardnerella species, as well as Fannyhessea vaginae, Lactobacillus crispatus and L. iners in BV.MethodsFrom 331 pregnant women from the Democratic Republic of the Congo, BV was diagnosed using Nugent scoring and a cervicovaginal lavage was used to quantify G. leopoldii, G. piotii, G. swidsinskii, G. vaginalis, F. vaginae, L. crispatus and L. iners by qPCR. Univariate associations between these species and clinical outcomes were assessed. A logistic regression model and ROC curves were calculated to determine the best diagnostic marker for BV.ResultsHere, L. iners (75.8%) was the most prevalent species and G. vaginalis (36.0%) the most common Gardnerella species. All investigated Gardnerella spp. were prevalent (50.9-57.9%) in women with (asymptomatic) BV. Univariate analysis revealed no significant associations with clinical symptoms of BV, while F. vaginae (positive Whiff test, high pH), G. vaginalis (high pH) and L. crispatus (low pH) were associated with signs of BV. G. piotii was associated with markers of urinary tract infection. Women with L. iners had higher odds of delivering preterm. ROC analyses showed that F. vaginae was the best marker for BV (AUC 0.81), and the combined model further increased the diagnostic performance (AUC 0.90).ConclusionAll Gardnerella species were involved in BV, although none were associated with the most important clinical symptoms of BV and none emerged as a superior molecular marker for BV. |
| format | Article |
| id | doaj-art-3ff479a0a9ec46e18f929617a6a13b19 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-3ff479a0a9ec46e18f929617a6a13b192025-01-17T05:10:36ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.15148841514884Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the CongoLisa Himschoot0Guy Mulinganya1Guy Mulinganya2Guy Mulinganya3Tess Rogier4Ghislain Bisimwa5Ghislain Bisimwa6Freddy Kampara7Freddy Kampara8Yvette Kujirakwinja9Yvette Kujirakwinja10Jules Mongane11Jules Mongane12Innocent Mubalama13Steven Callens14Mario Vaneechoutte15Piet Cools16Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumFaculty of Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of CongoDepartment of Obstetrics and Gynecology, Hôpital Provincial Général de Référence de Bukavu, Bukavu, Democratic Republic of CongoDepartment of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumDepartment of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumFaculty of Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of CongoDepartment of Obstetrics and Gynecology, Hôpital Provincial Général de Référence de Bukavu, Bukavu, Democratic Republic of CongoFaculty of Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of CongoDepartment of Obstetrics and Gynecology, Hôpital Provincial Général de Référence de Bukavu, Bukavu, Democratic Republic of CongoFaculty of Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of CongoDepartment of Obstetrics and Gynecology, Hôpital Provincial Général de Référence de Bukavu, Bukavu, Democratic Republic of CongoFaculty of Medicine, Catholic University of Bukavu, Bukavu, Democratic Republic of CongoDepartment of Obstetrics and Gynecology, Hôpital Provincial Général de Référence de Bukavu, Bukavu, Democratic Republic of CongoDepartment of Obstetrics and Gynecology, Hôpital Provincial Général de Référence de Bukavu, Bukavu, Democratic Republic of CongoDepartment of Obstetrics and Gynecology, Hôpital Provincial Général de Référence de Bukavu, Bukavu, Democratic Republic of CongoDepartment of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumDepartment of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, BelgiumBackgroundGardnerella is a key pathogen in bacterial vaginosis (BV), but the role of the different Gardnerella species remains unclear. We investigated the role of four Gardnerella species, as well as Fannyhessea vaginae, Lactobacillus crispatus and L. iners in BV.MethodsFrom 331 pregnant women from the Democratic Republic of the Congo, BV was diagnosed using Nugent scoring and a cervicovaginal lavage was used to quantify G. leopoldii, G. piotii, G. swidsinskii, G. vaginalis, F. vaginae, L. crispatus and L. iners by qPCR. Univariate associations between these species and clinical outcomes were assessed. A logistic regression model and ROC curves were calculated to determine the best diagnostic marker for BV.ResultsHere, L. iners (75.8%) was the most prevalent species and G. vaginalis (36.0%) the most common Gardnerella species. All investigated Gardnerella spp. were prevalent (50.9-57.9%) in women with (asymptomatic) BV. Univariate analysis revealed no significant associations with clinical symptoms of BV, while F. vaginae (positive Whiff test, high pH), G. vaginalis (high pH) and L. crispatus (low pH) were associated with signs of BV. G. piotii was associated with markers of urinary tract infection. Women with L. iners had higher odds of delivering preterm. ROC analyses showed that F. vaginae was the best marker for BV (AUC 0.81), and the combined model further increased the diagnostic performance (AUC 0.90).ConclusionAll Gardnerella species were involved in BV, although none were associated with the most important clinical symptoms of BV and none emerged as a superior molecular marker for BV.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1514884/fullbacterial vaginosisGardnerellamolecular diagnosispreterm birthlow birthweightDemocratic Republic of the Congo |
| spellingShingle | Lisa Himschoot Guy Mulinganya Guy Mulinganya Guy Mulinganya Tess Rogier Ghislain Bisimwa Ghislain Bisimwa Freddy Kampara Freddy Kampara Yvette Kujirakwinja Yvette Kujirakwinja Jules Mongane Jules Mongane Innocent Mubalama Steven Callens Mario Vaneechoutte Piet Cools Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo Frontiers in Cellular and Infection Microbiology bacterial vaginosis Gardnerella molecular diagnosis preterm birth low birthweight Democratic Republic of the Congo |
| title | Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo |
| title_full | Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo |
| title_fullStr | Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo |
| title_full_unstemmed | Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo |
| title_short | Prevalence and clinical correlates of Gardnerella spp., Fannyhessea vaginae, Lactobacillus crispatus and L. iners in pregnant women in Bukavu, Democratic Republic of the Congo |
| title_sort | prevalence and clinical correlates of gardnerella spp fannyhessea vaginae lactobacillus crispatus and l iners in pregnant women in bukavu democratic republic of the congo |
| topic | bacterial vaginosis Gardnerella molecular diagnosis preterm birth low birthweight Democratic Republic of the Congo |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1514884/full |
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