GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell Carcinoma

Background: Oral squamous cell carcinoma (OSCC) is the most prevalent malignancy and represents more than 91.5% of all oral malignancies. glucose transporter-1 (GLUT-1), a glucose transporter, increases the intake of glucose for use in aerobic glycolysis for quick cell division. Another protein that...

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Main Authors: Abeer Salah Salman, Layla Sabri Yas
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-01-01
Series:Medical Journal of Babylon
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Online Access:https://doi.org/10.4103/MJBL.MJBL_710_23
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author Abeer Salah Salman
Layla Sabri Yas
author_facet Abeer Salah Salman
Layla Sabri Yas
author_sort Abeer Salah Salman
collection DOAJ
description Background: Oral squamous cell carcinoma (OSCC) is the most prevalent malignancy and represents more than 91.5% of all oral malignancies. glucose transporter-1 (GLUT-1), a glucose transporter, increases the intake of glucose for use in aerobic glycolysis for quick cell division. Another protein that helps tumor cells survive is carbonic anhydrase IX (CAIX). Objectives: The aims of this study were to evaluate the expression of GLUT-1 and CAIX in OSCC patients and determine how these proteins’ expression correlated with histopathological grading and clinical staging. Materials and Methods: 24 specimens of OSCC were carefully chosen during the period of 2014–2017. Histological grade and TNM (T: tumor, N: extent of spread to the lymph node, M: presence of metastasis) clinical staging were done. The immunohistochemistry procedure was completed by the ordinary procedure for paraffin-embedded tissue blocks. Results: The clinical stage of OSCC was observed to significantly correlate with the expression of GLUT-1 and CAIX (P = 0.043 and P = 0.007, respectively), whereas an insignificant correlation of these proteins with histopathological grade was observed. Stage IV patients have higher GLUT-1 expression. This expression was mainly observed in the membrane and cytoplasm of tumor cells. Regarding CAIX, the majority of tumor cells have combined nucleus and cytoplasmic staining. Conclusion: Positive expression of GLUT-1 and CAIX relates significantly to pTNM staging and insignificantly to histological grade of OSCC. As a result, the expression of GLUT-1 may be used as a biomarker for those who have OSCC.
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spelling doaj-art-3fdfcfe4f6f34ca9b0ee89b8d2cf0a682025-08-20T03:49:46ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602025-01-0122112913510.4103/MJBL.MJBL_710_23GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell CarcinomaAbeer Salah SalmanLayla Sabri YasBackground: Oral squamous cell carcinoma (OSCC) is the most prevalent malignancy and represents more than 91.5% of all oral malignancies. glucose transporter-1 (GLUT-1), a glucose transporter, increases the intake of glucose for use in aerobic glycolysis for quick cell division. Another protein that helps tumor cells survive is carbonic anhydrase IX (CAIX). Objectives: The aims of this study were to evaluate the expression of GLUT-1 and CAIX in OSCC patients and determine how these proteins’ expression correlated with histopathological grading and clinical staging. Materials and Methods: 24 specimens of OSCC were carefully chosen during the period of 2014–2017. Histological grade and TNM (T: tumor, N: extent of spread to the lymph node, M: presence of metastasis) clinical staging were done. The immunohistochemistry procedure was completed by the ordinary procedure for paraffin-embedded tissue blocks. Results: The clinical stage of OSCC was observed to significantly correlate with the expression of GLUT-1 and CAIX (P = 0.043 and P = 0.007, respectively), whereas an insignificant correlation of these proteins with histopathological grade was observed. Stage IV patients have higher GLUT-1 expression. This expression was mainly observed in the membrane and cytoplasm of tumor cells. Regarding CAIX, the majority of tumor cells have combined nucleus and cytoplasmic staining. Conclusion: Positive expression of GLUT-1 and CAIX relates significantly to pTNM staging and insignificantly to histological grade of OSCC. As a result, the expression of GLUT-1 may be used as a biomarker for those who have OSCC.https://doi.org/10.4103/MJBL.MJBL_710_23caixglut-1immunohistochemistrysquamous cell carcinoma
spellingShingle Abeer Salah Salman
Layla Sabri Yas
GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell Carcinoma
Medical Journal of Babylon
caix
glut-1
immunohistochemistry
squamous cell carcinoma
title GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell Carcinoma
title_full GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell Carcinoma
title_fullStr GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell Carcinoma
title_full_unstemmed GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell Carcinoma
title_short GLUT-1 and Carbonic Anhydrase IX Immunoexpression in Oral Squamous Cell Carcinoma
title_sort glut 1 and carbonic anhydrase ix immunoexpression in oral squamous cell carcinoma
topic caix
glut-1
immunohistochemistry
squamous cell carcinoma
url https://doi.org/10.4103/MJBL.MJBL_710_23
work_keys_str_mv AT abeersalahsalman glut1andcarbonicanhydraseiximmunoexpressioninoralsquamouscellcarcinoma
AT laylasabriyas glut1andcarbonicanhydraseiximmunoexpressioninoralsquamouscellcarcinoma