Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation
Morphological changes in leukocytes are valuable markers for diseases and immune responses. In our earlier work, we presented Cellytics, a device that uses lens-free shadow imaging technology (LSIT) to monitor natural killer cell activity. Here, we present an improved Cellytics system that has been...
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MDPI AG
2025-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/3/143 |
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| author | Hojin Cheon Samir Kumar Inha Lee Sanghoon Shin Hyeji Jang Young-Sun Lee Myung-Hyun Nam Hyun Sik Jun Sungkyu Seo |
| author_facet | Hojin Cheon Samir Kumar Inha Lee Sanghoon Shin Hyeji Jang Young-Sun Lee Myung-Hyun Nam Hyun Sik Jun Sungkyu Seo |
| author_sort | Hojin Cheon |
| collection | DOAJ |
| description | Morphological changes in leukocytes are valuable markers for diseases and immune responses. In our earlier work, we presented Cellytics, a device that uses lens-free shadow imaging technology (LSIT) to monitor natural killer cell activity. Here, we present an improved Cellytics system that has been upgraded to a four-channel configuration to achieve higher throughput while maintaining robust reproducibility for rapid and cost-effective leukocyte analysis. The performance of this multi-channel Cellytics system was improved through refinements to the micro-pinhole chip. Etched pinholes provided better image resolution and clarity compared to drilled pinholes. To stimulate leukocytes, we used an activation stimulator cocktail (ASC) and quantified the resulting morphological changes using shadow-based metrics, including peak-to-peak distance (PPD) and maxima-to-minima standard deviation (MMD-SD). In addition, we developed a new leukocyte activation parameter (LAP) to specifically assess these activation-induced morphological changes. After ASC stimulation, leukocytes showed significantly increased PPD and LAP values and decreased MMD-SD compared to non-activated leukocytes. These results are consistent with the results of the flow cytometric analysis. These results emphasize the potential of Cellytics for the rapid and accurate assessment of leukocyte activation and provide a valuable tool for both clinical diagnostics and basic immunological research. |
| format | Article |
| id | doaj-art-3fdca3280d6a430e921ac2e21c6893f7 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj-art-3fdca3280d6a430e921ac2e21c6893f72025-08-20T03:43:37ZengMDPI AGBiosensors2079-63742025-02-0115314310.3390/bios15030143Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte ActivationHojin Cheon0Samir Kumar1Inha Lee2Sanghoon Shin3Hyeji Jang4Young-Sun Lee5Myung-Hyun Nam6Hyun Sik Jun7Sungkyu Seo8Department of Electronics and Information Engineering, Korea University, Sejong 30019, Republic of KoreaDepartment of Electronics and Information Engineering, Korea University, Sejong 30019, Republic of KoreaDepartment of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of KoreaDepartment of Electronics and Information Engineering, Korea University, Sejong 30019, Republic of KoreaDepartment of Electronics and Information Engineering, Korea University, Sejong 30019, Republic of KoreaDepartment of Gastroenterology and Hepatology, Guro Hospital, Korea University College of Medicine, Seoul 08308, Republic of KoreaDepartment of Laboratory Medicine, Anam Hospital, Korea University College of Medicine, Seoul 02841, Republic of KoreaDepartment of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of KoreaDepartment of Electronics and Information Engineering, Korea University, Sejong 30019, Republic of KoreaMorphological changes in leukocytes are valuable markers for diseases and immune responses. In our earlier work, we presented Cellytics, a device that uses lens-free shadow imaging technology (LSIT) to monitor natural killer cell activity. Here, we present an improved Cellytics system that has been upgraded to a four-channel configuration to achieve higher throughput while maintaining robust reproducibility for rapid and cost-effective leukocyte analysis. The performance of this multi-channel Cellytics system was improved through refinements to the micro-pinhole chip. Etched pinholes provided better image resolution and clarity compared to drilled pinholes. To stimulate leukocytes, we used an activation stimulator cocktail (ASC) and quantified the resulting morphological changes using shadow-based metrics, including peak-to-peak distance (PPD) and maxima-to-minima standard deviation (MMD-SD). In addition, we developed a new leukocyte activation parameter (LAP) to specifically assess these activation-induced morphological changes. After ASC stimulation, leukocytes showed significantly increased PPD and LAP values and decreased MMD-SD compared to non-activated leukocytes. These results are consistent with the results of the flow cytometric analysis. These results emphasize the potential of Cellytics for the rapid and accurate assessment of leukocyte activation and provide a valuable tool for both clinical diagnostics and basic immunological research.https://www.mdpi.com/2079-6374/15/3/143lens-free shadow imaging technologyleukocyte activationmulti-channel Cellyticsshadow parameterscell morphology |
| spellingShingle | Hojin Cheon Samir Kumar Inha Lee Sanghoon Shin Hyeji Jang Young-Sun Lee Myung-Hyun Nam Hyun Sik Jun Sungkyu Seo Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation Biosensors lens-free shadow imaging technology leukocyte activation multi-channel Cellytics shadow parameters cell morphology |
| title | Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation |
| title_full | Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation |
| title_fullStr | Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation |
| title_full_unstemmed | Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation |
| title_short | Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation |
| title_sort | multi channel cellytics for rapid and cost effective monitoring of leukocyte activation |
| topic | lens-free shadow imaging technology leukocyte activation multi-channel Cellytics shadow parameters cell morphology |
| url | https://www.mdpi.com/2079-6374/15/3/143 |
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