The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion
Life histories of oviparous species dictate high metabolic investment in the process of gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH), respectively. While it w...
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eLife Sciences Publications Ltd
2024-12-01
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| Online Access: | https://elifesciences.org/articles/96344 |
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| author | Lian Hollander-Cohen Omer Cohen Miriam Shulman Tomer Aiznkot Pierre Fontanaud Omer Revah Patrice Mollard Matan Golan Berta Levavi-Sivan |
| author_facet | Lian Hollander-Cohen Omer Cohen Miriam Shulman Tomer Aiznkot Pierre Fontanaud Omer Revah Patrice Mollard Matan Golan Berta Levavi-Sivan |
| author_sort | Lian Hollander-Cohen |
| collection | DOAJ |
| description | Life histories of oviparous species dictate high metabolic investment in the process of gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing hormone (GnRH), oversees both functions, the generation of loss-of-function fish challenged this view. Here, we reveal that the satiety hormone cholecystokinin (CCK) is the primary regulator of this axis in zebrafish. We found that FSH cells express a CCK receptor, and our findings demonstrate that mutating this receptor results in a severe hindrance to ovarian development. Additionally, it causes a complete shutdown of both gonadotropins secretion. Using in-vivo and ex-vivo calcium imaging of gonadotrophs, we show that GnRH predominantly activates LH cells, whereas FSH cells respond to CCK stimulation, designating CCK as the bona fide FSH secretagogue. These findings indicate that the control of gametogenesis in fish was placed under different neural circuits, that are gated by CCK. |
| format | Article |
| id | doaj-art-3fd03507a3c44dd19240c7a5f3c5bc33 |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj-art-3fd03507a3c44dd19240c7a5f3c5bc332024-12-24T09:11:55ZengeLife Sciences Publications LtdeLife2050-084X2024-12-011310.7554/eLife.96344The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretionLian Hollander-Cohen0https://orcid.org/0000-0002-1590-5932Omer Cohen1Miriam Shulman2Tomer Aiznkot3Pierre Fontanaud4Omer Revah5Patrice Mollard6https://orcid.org/0000-0002-2324-7589Matan Golan7Berta Levavi-Sivan8https://orcid.org/0000-0002-0183-9524Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, IsraelInstitute of Functional Genomics, University of Montpellier, Montpellier, France; BioCampus Montpellier, University of Montpellier, Montpellier, FranceThe Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelInstitute of Functional Genomics, University of Montpellier, Montpellier, France; BioCampus Montpellier, University of Montpellier, Montpellier, FranceDepartment of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel; Department of Poultry and Aquaculture, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Rishon Letziyon, IsraelDepartment of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, IsraelLife histories of oviparous species dictate high metabolic investment in the process of gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing hormone (GnRH), oversees both functions, the generation of loss-of-function fish challenged this view. Here, we reveal that the satiety hormone cholecystokinin (CCK) is the primary regulator of this axis in zebrafish. We found that FSH cells express a CCK receptor, and our findings demonstrate that mutating this receptor results in a severe hindrance to ovarian development. Additionally, it causes a complete shutdown of both gonadotropins secretion. Using in-vivo and ex-vivo calcium imaging of gonadotrophs, we show that GnRH predominantly activates LH cells, whereas FSH cells respond to CCK stimulation, designating CCK as the bona fide FSH secretagogue. These findings indicate that the control of gametogenesis in fish was placed under different neural circuits, that are gated by CCK.https://elifesciences.org/articles/96344CCKfshpituitaryreproduction |
| spellingShingle | Lian Hollander-Cohen Omer Cohen Miriam Shulman Tomer Aiznkot Pierre Fontanaud Omer Revah Patrice Mollard Matan Golan Berta Levavi-Sivan The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion eLife CCK fsh pituitary reproduction |
| title | The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion |
| title_full | The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion |
| title_fullStr | The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion |
| title_full_unstemmed | The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion |
| title_short | The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion |
| title_sort | satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion |
| topic | CCK fsh pituitary reproduction |
| url | https://elifesciences.org/articles/96344 |
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