Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalcone
In this study, a straightforward spectrophotometric method was developed for quantifying the total content of chalcones in a sample. The method exhibits linearity, accuracy, precision, repeatability, and enables the estimation of total chalcone content in trans-chalcone equivalents for a sample dilu...
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2025-06-01
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| author | J.R.S. Botelho A. Caeiro H.C. Sousa M.E.M. Braga M.G. Campos |
| author_facet | J.R.S. Botelho A. Caeiro H.C. Sousa M.E.M. Braga M.G. Campos |
| author_sort | J.R.S. Botelho |
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| description | In this study, a straightforward spectrophotometric method was developed for quantifying the total content of chalcones in a sample. The method exhibits linearity, accuracy, precision, repeatability, and enables the estimation of total chalcone content in trans-chalcone equivalents for a sample diluted in carbon tetrachloride and added to antimony pentachloride. The analytical wavelength was determined to be 390 nm. Linearity was observed within the concentration range of 0.3 to 17.6 µg/mL of trans-chalcone in CCl4, with a R2 of 0.9994 and a random dispersion of the residual concentrations. In the accuracy study, recoveries ranged from 98 to 102 %. In the inter-day precision study, the compared data showed no statistically significant differences based on p-values from Student's t-test and the two-tailed Kruskal-Wallis test. In the repeatability study, the coefficients of variation were 1.92 % and 2.08 %. Additionally, this method is specific for trans-chalcone in the presence of the flavanol (+)-catechin, the flavone luteolin, and the flavonol quercetin. The total content of chalcone was estimated for plant extracts in trans-chalcone equivalents (tCe). The total content of chalcones in these extracts ranged from non-detected up to 22 % of tCe. Therefore, this method can be used for the initial estimation of the overall chalcone content. |
| format | Article |
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| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| spelling | doaj-art-3faf8a8510214b418dbdec4dc0f6af552025-01-01T05:10:24ZengElsevierMethodsX2215-01612025-06-0114103119Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalconeJ.R.S. Botelho0A. Caeiro1H.C. Sousa2M.E.M. Braga3M.G. Campos4University of Coimbra, CERES, Department of Chemical Engineering, 3030-790 Coimbra, PortugalCentre for Functional Ecology, Laboratory Associate TERRA, Department of Life Sciences, University of Coimbra, Coimbra, PortugalUniversity of Coimbra, CERES, Department of Chemical Engineering, 3030-790 Coimbra, PortugalUniversity of Coimbra, CERES, Department of Chemical Engineering, 3030-790 Coimbra, Portugal; Corresponding authors at: University of Coimbra, CERES, Department of Chemical Engineering, 3030-790 Coimbra, Portugal.Observatory of Drug-Herb Interactions, Faculty of Pharmacy, University of Coimbra, Health Science Campus, Azinhaga Santa Comba, Coimbra, Portugal; Coimbra Chemistry Centre (CQC, FCT Unit 313) (FCTUC), University of Coimbra, Rua Larga, Coimbra, Portugal; Corresponding authors at: Observatory of Drug-Herb Interactions, Faculty of Pharmacy, University of Coimbra, Health Science Campus, Azinhaga Santa Comba, Coimbra, Portugal.In this study, a straightforward spectrophotometric method was developed for quantifying the total content of chalcones in a sample. The method exhibits linearity, accuracy, precision, repeatability, and enables the estimation of total chalcone content in trans-chalcone equivalents for a sample diluted in carbon tetrachloride and added to antimony pentachloride. The analytical wavelength was determined to be 390 nm. Linearity was observed within the concentration range of 0.3 to 17.6 µg/mL of trans-chalcone in CCl4, with a R2 of 0.9994 and a random dispersion of the residual concentrations. In the accuracy study, recoveries ranged from 98 to 102 %. In the inter-day precision study, the compared data showed no statistically significant differences based on p-values from Student's t-test and the two-tailed Kruskal-Wallis test. In the repeatability study, the coefficients of variation were 1.92 % and 2.08 %. Additionally, this method is specific for trans-chalcone in the presence of the flavanol (+)-catechin, the flavone luteolin, and the flavonol quercetin. The total content of chalcone was estimated for plant extracts in trans-chalcone equivalents (tCe). The total content of chalcones in these extracts ranged from non-detected up to 22 % of tCe. Therefore, this method can be used for the initial estimation of the overall chalcone content.http://www.sciencedirect.com/science/article/pii/S2215016124005703ChalconeUV-VIS spectrophotometryTotal content estimation |
| spellingShingle | J.R.S. Botelho A. Caeiro H.C. Sousa M.E.M. Braga M.G. Campos Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalcone MethodsX Chalcone UV-VIS spectrophotometry Total content estimation |
| title | Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalcone |
| title_full | Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalcone |
| title_fullStr | Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalcone |
| title_full_unstemmed | Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalcone |
| title_short | Development and validation of a UV–Vis spectrophotometric method for estimation of total content of chalcone |
| title_sort | development and validation of a uv vis spectrophotometric method for estimation of total content of chalcone |
| topic | Chalcone UV-VIS spectrophotometry Total content estimation |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124005703 |
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